Assessment and comparison of neural morphology through metrical feature extraction and analysis in neuron and neuron–glia cultures

The morphology of dissociated single cerebellar Purkinje cells obtained from wild-type P1 CD1 mice was assessed in the absence and in the presence of glia. A dedicated noninvasive technique based on optical microscopy was developed. Image processing algorithms were implemented to extract metrical features characterizing cell structure and dendritic arborization. The morphological features were analyzed in order to identify quantitative differences in Purkinje cell morphology due to interactions with astrocytes.

[1]  R. Donato,et al.  S100: a multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles. , 2001, The international journal of biochemistry & cell biology.

[2]  Matthew J. McAuliffe,et al.  Medical Image Processing, Analysis and Visualization in clinical research , 2001, Proceedings 14th IEEE Symposium on Computer-Based Medical Systems. CBMS 2001.

[3]  J. B. Stankovic,et al.  Fractal analysis of the laminar organization of spinal cord neurons , 2005, Journal of Neuroscience Methods.

[4]  J. Chan,et al.  The geometric and spatial constraints of the microenvironment induce oligodendrocyte differentiation , 2008, Proceedings of the National Academy of Sciences.

[5]  P Sterling,et al.  Retinal neurons and vessels are not fractal but space‐filling , 1995, The Journal of comparative neurology.

[6]  M. Alexander,et al.  Principles of Neural Science , 1981 .

[7]  Pablo Blinder,et al.  Functional Topology Classification of Biological Computing Networks , 2005, Natural Computing.

[8]  M. L. Martins,et al.  Fractal patterns for dendrites and axon terminals , 1996 .

[9]  B. Marchetti,et al.  Cross-talk signals in the CNS: role of neurotrophic and hormonal factors, adhesion molecules and intercellular signaling agents in luteinizing hormone-releasing hormone (LHRH)-astroglial interactive network. , 1997, Frontiers in bioscience : a journal and virtual library.

[10]  Shiaoping C. Zhu,et al.  A Glia-Derived Signal Regulating Neuronal Differentiation , 2000, The Journal of Neuroscience.

[11]  Michael D. Abràmoff,et al.  Image processing with ImageJ , 2004 .

[12]  K. Sato,et al.  Optical mapping of neural network activity in chick spinal cord at an intermediate stage of embryonic development. , 1999, Journal of neurophysiology.

[13]  Pierre J. Magistretti,et al.  The tripartite synapse: glia in synaptic transmission , 2002 .

[14]  Mu-ming Poo,et al.  ATP Released by Astrocytes Mediates Glutamatergic Activity-Dependent Heterosynaptic Suppression , 2003, Neuron.

[15]  P. Thuras,et al.  Purkinje Cell Size Is Reduced in Cerebellum of Patients with Autism , 2002, Cellular and Molecular Neurobiology.

[16]  Jaap van Pelt,et al.  Measures for quantifying dendritic arborizations , 2002, Network.

[17]  Herbert F Jelinek,et al.  Neurons and fractals: how reliable and useful are calculations of fractal dimensions? , 1998, Journal of Neuroscience Methods.

[18]  G.A. Silva,et al.  High Throughput Algorithms for Mapping the Topology of Neuronal and Glial Networks , 2005, Conference Proceedings. 2nd International IEEE EMBS Conference on Neural Engineering, 2005..

[19]  P. Haydon.  Glia: listening and talking to the synapse , 2001, Nature Reviews Neuroscience.

[20]  Cristopher M Niell,et al.  Live optical imaging of nervous system development. , 2004, Annual review of physiology.

[21]  Benoit B. Mandelbrot,et al.  Fractal Geometry of Nature , 1984 .

[22]  Y. Hirabayashi,et al.  An improved method for culturing cerebellar Purkinje cells with differentiated dendrites under a mixed monolayer setting. , 1998, Brain research. Brain research protocols.

[23]  H. Althaus,et al.  Glial cells as targets and producers of neurotrophins. , 2000, International review of cytology.

[24]  C. D. Murray.  THE PHYSIOLOGICAL PRINCIPLE OF MINIMUM WORK APPLIED TO THE ANGLE OF BRANCHING OF ARTERIES , 1926, The Journal of general physiology.

[25]  H. E. Stanley,et al.  Determination of fractal dimension of physiologically characterized neurons in two and three dimensions , 1995, Journal of Neuroscience Methods.

[26]  D. Ristanovic,et al.  The Sholl analysis of neuronal cell images: semi-log or log-log method? , 2007, Journal of theoretical biology.

[27]  A. Vaheri,et al.  Laminin is produced by early rat astrocytes in primary culture , 1983, The Journal of cell biology.

[28]  B. Barres,et al.  Role for glia in synaptogenesis , 2004, Glia.

[29]  Janet K. Kern,et al.  Purkinje cell vulnerability and autism: a possible etiological connection , 2003, Brain and Development.

[30]  Katsuhiko Ono,et al.  Neuron-glia interrelations during migration of Purkinje cells in the mouse embryonic cerebellum , 1996, International Journal of Developmental Neuroscience.

[31]  Amir C. Akhavan,et al.  Possible role of S-100 in glia—Neuronal signalling involved in activity-dependent plasticity in the developing mammalian cortex , 1993, Journal of Chemical Neuroanatomy.

[32]  F. Seil,et al.  Interactions between cerebellar Purkinje cells and their associated astrocytes. , 2001, Histology and histopathology.

[33]  H F Jelinek,et al.  Use of fractal theory in neuroscience: methods, advantages, and potential problems. , 2001, Methods.

[34]  A. Zimmerman,et al.  Neuroglial activation and neuroinflammation in the brain of patients with autism , 2005, Annals of neurology.

[35]  T. Kemper,et al.  Neuroanatomic observations of the brain in autism: a review and future directions , 2005, International Journal of Developmental Neuroscience.

[36]  A. Araque,et al.  Dynamic signaling between astrocytes and neurons. , 2001, Annual review of physiology.

[37]  Toshiaki Takeda,et al.  Fractal dimension of dendritic tree of cerebellar Purkinje cell during onto- and phylogenetic development , 1992, Neuroscience Research.

[38]  Toshihide Tabata,et al.  A reliable method for culture of dissociated mouse cerebellar cells enriched for Purkinje neurons , 2000, Journal of Neuroscience Methods.

[39]  Amir Ayali,et al.  A two-phase growth strategy in cultured neuronal networks as reflected by the distribution of neurite branching angles. , 2005, Journal of neurobiology.

[40]  Sholl Da.  Dendritic organization in the neurons of the visual and motor cortices of the cat. , 1953 .

[41]  G. Wilkin,et al.  Neural cell culture: a practical approach , 1995 .

[42]  Dmitri B Chklovskii,et al.  Synaptic Connectivity and Neuronal Morphology Two Sides of the Same Coin , 2004, Neuron.

[43]  Dušan Ristanović,et al.  Fractality of dendritic arborization of spinal cord neurons , 2006, Neuroscience Letters.