Inducible nitric oxide synthase and nitric oxide production by oligodendrocytes

It has been previously demonstrated that microglia and astrocytes produce micromolar amounts of nitric oxide in vitro. In this study, we demonstrate that primary rat oligodendrocytes can be stimulated to produce iNOS mRNA as detected by Northern blot and in situ hybridization analysis and a 131‐kDa iNOS protein by Western blot analysis; protein was also detected in cells by single‐ and double‐label immunohistochemistry for iNOS and the oligodendrocyte‐specific marker CNPase. NO/NOS are produced as a consequence of activation of the gene encoding the inducible nitric oxide synthase as determined by inhibition with actinomycin D and cyclohexamide. The iNOS is functional, leading to calcium/calmodulin‐independent NO production in these in vitro cultures. J. Neurosci. Res. 48:372–384, 1997. © 1997 Wiley‐Liss, Inc.

[1]  C. Lowenstein,et al.  Nitric oxide inhibits viral replication in murine myocarditis. , 1996, The Journal of clinical investigation.

[2]  H. Hartung,et al.  Secretion of nitrite by Schwann cells and its effect on T-cell activation in vitro. , 1996, Cellular immunology.

[3]  O. Bagasra,et al.  Activation of the inducible form of nitric oxide synthase in the brains of patients with multiple sclerosis. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[4]  J. Merrill,et al.  Human immunodeficiency virus 1 envelope proteins induce interleukin 1, tumor necrosis factor alpha, and nitric oxide in glial cultures derived from fetal, neonatal, and adult human brain , 1995, The Journal of experimental medicine.

[5]  M. Geffard,et al.  Indirect evidence for nitric oxide involvement in multiple sclerosis by characterization of circulating antibodies directed against conjugated S-nitrosocysteine , 1995, Journal of Neuroimmunology.

[6]  H. Vinters,et al.  Nitric oxide induces necrotic but not apoptotic cell death in oligodendrocytes , 1995, Neuroscience.

[7]  J. Bolaños,et al.  Evidence for increased nitric oxide production in multiple sclerosis. , 1995, Journal of neurology, neurosurgery, and psychiatry.

[8]  D. Grzybicki,et al.  Glial Cells as Nitric Oxide Sources and Targets , 1995 .

[9]  D. Hanley,et al.  Induction of nitric oxide synthase in demyelinating regions of multiple sclerosis brains , 1994, Annals of neurology.

[10]  S. Barnes,et al.  Nitric oxide regulation of superoxide and peroxynitrite-dependent lipid peroxidation. Formation of novel nitrogen-containing oxidized lipid derivatives. , 1994, The Journal of biological chemistry.

[11]  J. Bolaños,et al.  Nitric Oxide‐Mediated Inhibition of the Mitochondrial Respiratory Chain in Cultured Astrocytes , 1994, Journal of neurochemistry.

[12]  L. Ignarro,et al.  Nitric oxide as a potential pathological mechanism in demyelination: Its differential effects on primary glial cellsin vitro , 1994, Neuroscience.

[13]  W. Sessa.  The nitric oxide synthase family of proteins. , 1994, Journal of vascular research.

[14]  D. Reis,et al.  Cloning and expression of inducible nitric oxide synthase from rat astrocytes , 1994, Journal of neuroscience research.

[15]  A. MacKenzie-Graham,et al.  Differential sensitivity to nitric oxide in immortalized, cloned murine oligodendrocyte cell lines. , 1994, Developmental neuroscience.

[16]  T. Dawson,et al.  gp120 neurotoxicity in primary cortical cultures. , 1994, Advances in neuroimmunology.

[17]  L. Ignarro,et al.  Inducible nitric oxide synthase from a rat alveolar macrophage cell line is inhibited by nitric oxide. , 1993, Journal of immunology.

[18]  L. Ignarro,et al.  Oxidation of nitric oxide in aqueous solution to nitrite but not nitrate: comparison with enzymatically formed nitric oxide from L-arginine. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[19]  L. Ignarro,et al.  Microglial cell cytotoxicity of oligodendrocytes is mediated through nitric oxide. , 1993, Journal of immunology.

[20]  D. Reis,et al.  Synthesis of nitric oxide in CNS glial cells , 1993, Trends in Neurosciences.

[21]  A. Nussler,et al.  Inflammation, immunoregulation, and inducible nitric oxide synthase , 1993, Journal of leukocyte biology.

[22]  Y. Vodovotz,et al.  Mechanisms of suppression of macrophage nitric oxide release by transforming growth factor beta , 1993, The Journal of experimental medicine.

[23]  G. Nisticó,et al.  HIV gp120 glycoprotein stimulates the inducible isoform of no synthase in human cultured astrocytoma cells. , 1993, Biochemical and biophysical research communications.

[24]  D. Dickson,et al.  Induction of nitric oxide synthase activity in human astrocytes by interleukin-1 beta and interferon-gamma. , 1993, Journal of neuroimmunology.

[25]  G. Nisticó,et al.  Cytokine-induced nitric oxide generation by cultured astrocytoma cells involves Ca(++)-calmodulin-independent NO-synthase. , 1993, Biochemical and biophysical research communications.

[26]  D. Reis,et al.  Induction of calcium-independent nitric oxide synthase activity in primary rat glial cultures. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[27]  Klaus V. Toyka,et al.  Inflammatory mediators in demyelinating disorders of the CNS and PNS , 1992, Journal of Neuroimmunology.

[28]  M. Simmons,et al.  Induction of Nitric Oxide Synthase in Glial Cells , 1992, Journal of neurochemistry.

[29]  S. Snyder,et al.  Cloned and expressed macrophage nitric oxide synthase contrasts with the brain enzyme. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[30]  L. Ignarro,et al.  Nitric oxide synthase from cerebellum catalyzes the formation of equimolar quantities of nitric oxide and citrulline from L-arginine. , 1992, Biochemical and biophysical research communications.

[31]  H. Hartung,et al.  Production of nitrite by neonatal rat microglial cells/brain macrophages. , 1992, Cellular Immunology.

[32]  Terry D. Lee,et al.  Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. , 1992, Science.

[33]  T. Billiar,et al.  Effect of exogenous and endogenous nitric oxide on mitochondrial respiration of rat hepatocytes. , 1991, The American journal of physiology.

[34]  L. Ignarro.  Biosynthesis and metabolism of endothelium-derived nitric oxide. , 1990, Annual review of pharmacology and toxicology.

[35]  S. Snyder,et al.  Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[36]  J. Drapier,et al.  Differentiation of murine macrophages to express nonspecific cytotoxicity for tumor cells results in L-arginine-dependent inhibition of mitochondrial iron-sulfur enzymes in the macrophage effector cells. , 1988, Journal of immunology.