Ultrasensitive photoelectrochemical biosensing based on biocatalytic deposition

Abstract The coupling of biocatalytic precipitation (BCP) to photoelectrochemical (PEC) biosensing for the development of rapid PEC sensors with ultrahigh sensitivity and excellent anti-interference ability is successfully realized. Horseradish peroxidase (HRP) was immobilized on the matrix of TiO 2 /CdS and 4-chloro-1-naphthol (4-CN) was chosen for the BCP-amplified PEC detection of H 2 O 2 . The HRP accelerated oxidation of 4-CN by H 2 O 2 would yield insoluble product on the photoelectrode surface, on the basis of which the photocurrent be influenced. The detection limit of 5.0 × 10 −10  M (S/N = 3) for the determination of H 2 O 2 was achieved.

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