Outcomes of a research‐driven laboratory and literature course designed to enhance undergraduate contributions to original research

This work describes outcomes of a research‐driven advanced microbiology laboratory and literature research course intended to enhance undergraduate preparation for and contributions to original research. The laboratory section was designed to teach fundamental biochemistry and molecular biology techniques in the context of an original research project. Site‐directed mutants of a gene of interest were constructed, and the effects of mutations on the resulting enzymes were analyzed. Students were also introduced to the literature surrounding their project, electronic literature databases, and preparation of computer‐generated slides for oral presentations. Student progress was evaluated through a laboratory report written as scientific manuscript, an oral presentation, a 10‐page written review, and an essay examination. In the semester following the laboratory course, four of the 14 undergraduates joined the host laboratory to continue their projects as individual undergraduate researchers. Quantifiable outcomes of the course and subsequent undergraduate research included i) production of eight new site‐directed mutants and preliminary characterization of the corresponding enzymes, ii) training of four individual undergraduate researchers prior to joining the laboratory, iii) publication of a manuscript with results from two undergraduate researchers, and iv) presentation of two posters with undergraduate co‐authors at a national meeting. This research‐driven approach may be applicable to enhance undergraduate contributions to other original research projects that have defined goals achievable within the timeframe of a single semester.

[1]  Sarah M Boomer,et al.  Bacterial Diversity Studies Using the 16S rRNA Gene Provide a Powerful Research-Based Curriculum for Molecular Biology Laboratory. , 2002, Microbiology education.

[2]  Jane Slezak Student-Inspired Undergraduate Research , 1999 .

[3]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[4]  Ellis Bell,et al.  Recommended curriculum for a program in biochemistry and molecular biology , 2003 .

[5]  B. Pierson,et al.  Molecular Characterization of Novel Red Green Nonsulfur Bacteria from Five Distinct Hot Spring Communities in Yellowstone National Park , 2002, Applied and Environmental Microbiology.

[6]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[7]  M. Rasche,et al.  Mechanism for the enzymatic formation of 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate during the biosynthesis of methanopterin. , 1998, Biochemistry.

[8]  N. W. Bower,et al.  Teaching Experimental Design Using a GC-MS Analysis of Cocaine on Money: A Cross-Disciplinary Laboratory , 2002 .

[9]  Donald J. Wink,et al.  Development of a biochemistry laboratory course with a project‐oriented goal , 2003 .

[10]  M. Rasche,et al.  Purification, Overproduction, and Partial Characterization of β-RFAP Synthase, a Key Enzyme in the Methanopterin Biosynthesis Pathway† , 2002, Journal of bacteriology.

[11]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[12]  Rebecca Rawls AN UNDERGRADUATE CHAMPION: University of Arizona's Mike Doyle wants more undergraduates to do chemistry research , 2002 .

[13]  H. Yanagi,et al.  Overexpression of Trigger Factor Prevents Aggregation of Recombinant Proteins in Escherichia coli , 2000, Applied and Environmental Microbiology.

[14]  T. Bobik,et al.  Unusual coenzymes of methanogenesis. , 1985, Annual review of biochemistry.

[15]  Thomas J. Wenzel A/C Educator: Undergraduate research: A capstone learning experience. , 2000 .

[16]  L. L. Wright,et al.  Techniques in Chemistry: The Centerpiece of a Research-Oriented Curriculum. , 2002 .

[17]  T. Bobik,et al.  UNUSUAL COENZYMES OF METHANOGENESIS , 1990 .

[18]  J. Lichtenhan,et al.  Nanostructured chemicals : A new era in chemical technology , 2001 .

[19]  Wenzel.,et al.  Undergraduate research: a capstone learning experience , 2000, Analytical chemistry.

[20]  Aaron R. Hutchison,et al.  Research with first- and second-year undergraduates: A new model for undergraduate inquiry at research universities , 2002 .

[21]  Miss A.O. Penney (b) , 1974, The New Yale Book of Quotations.

[22]  Michael P. Doyle Academic Excellence¿The Role of Research , 2002 .

[23]  Division on Earth BIO2010: Transforming Undergraduate Education for Future Research Biologists , 2003 .