Hydration of polymeric components of cartilage--an infrared spectroscopic study on hyaluronic acid and chondroitin sulfate.

Hydrated polysaccharides are major constituents of cartilage and play an important role in its water-binding properties. Infrared (IR) spectroscopy and sorption isotherms have been used to investigate the hydration behavior of the glycosaminoglycans hyaluronic acid and chondroitin sulfate. IR-dichroism of the vibrational modes of the pyranose ring is found at relative humidities (RH) smaller than 84%. The IR-dichroism data for the vibrational modes of the pyranose ring have been analyzed with respect to the helical structure of these polysaccharides. The orientation vanishes at higher relative humidities (>84%), because a strong increase in the water uptake occurs in the observed sorption isotherms. Differences in the IR-absorbance of the O-H stretching mode of sorbed water between hyaluronic acid and chondroitin sulfate are shown to be caused by the additional hydration of the sulfate groups. The corresponding H-bonds are weaker than those of the hydration shell of the pyranose rings.

[1]  Heinz W. Siesler,et al.  Infrared and Raman spectroscopy of polymers , 1980 .

[2]  B. Casu,et al.  Infrared absorption and raman scattering of sulfate groups of heparin and related glycosaminoglycans in aqueous solution , 1978 .

[3]  S. A. Lee,et al.  Observation of a phase transition in wet-spun films of Na-hyaluronate. , 1992, Biopolymers.

[4]  H. Binder,et al.  Lyotropic Phase Behavior and Gel State Polymorphism of Phospholipids with Terminal Diene Groups: Infrared Measurements on Molecular Ordering in Lamellar and Hexagonal Phases , 1999 .

[5]  R. Giegé.  Application of infrared, Raman, and resonance Raman spectroscopy in biochemistry: par Frank S. Parker (Plenum Press, New-York, Londres) 1983, 550 pages , 1984 .

[6]  A. Brass,et al.  Secondary and tertiary structures of hyaluronan in aqueous solution, investigated by rotary shadowing-electron microscopy and computer simulation. Hyaluronan is a very efficient network-forming polymer. , 1991, The Biochemical journal.

[7]  N. Harrick,et al.  Internal reflection spectroscopy , 1968 .

[8]  B. Casu,et al.  Infrared spectra of glycosaminoglycans in deuterium oxide and deuterium chloride solution: Quantitative evaluation of uronic acid and acetamidodeoxyhexose moieties , 1978 .

[9]  S. Orr.  Infra-red spectroscopic studies of some polysaccharides. , 1954, Biochimica et biophysica acta.

[10]  J. Desbrières,et al.  Hydration of hyaluronic acid as a function of the counterion type and relative humidity , 1995 .

[11]  K. S. Dodgson,et al.  Infra-Red Spectra of Carbohydrate Sulphate Esters , 1959, Nature.

[12]  M. Mathews,et al.  Isomeric Chondroitin Sulphates , 1958, Nature.

[13]  J. Desbrières,et al.  Characterisation of sorbed water molecules on neutral and ionic polysaccharides. , 1996, International journal of biological macromolecules.

[14]  A. Maroudas,et al.  Methods in cartilage research , 1990 .

[15]  E. Atkins,et al.  Hyaluronic acid: a double-helical structure in the presence of potassium at low pH and found also with the cations ammonium, rubidium and caesium. , 1977, Journal of molecular biology.

[16]  J. Bartlett,et al.  Vibrational Spectroscopic Study of the Structure of Sodium Bis(2-ethylhexyl)sulfosuccinate Reverse Micelles and Water-in-Oil Microemulsions , 1995 .

[17]  E. Topp,et al.  Hydration in hyaluronic acid and its esters using differential scanning calorimetry , 1992 .

[18]  H. Binder,et al.  Hydration-Induced Gel States of the Dienic Lipid 1,2-Bis(2,4-octadecadienoyl)-sn-glycero-3-phosphorylcholine and Their Characterization Using Infrared Spectroscopy , 1997 .

[19]  N. K. ADAM,et al.  The Adsorption of Gases and Vapours , 1945, Nature.

[20]  S. Weng,et al.  Comparative Study on Structure of Solubilized Water in Reversed Micelles. 1. FT-IR Spectroscopic Evidence of Water/AOT/n-Heptane and Water/NaDEHP/n-Heptane Systems , 1998 .

[21]  F. Parker.  Applications of Infrared, Raman, and Resonance Raman Spectroscopy in Biochemistry , 1983 .

[22]  J. Koenig,et al.  Polarized infrared spectra of crystalline glycosaminoglycans. , 1976, Carbohydrate research.

[23]  W Gründer,et al.  Action of Compression and Cations on the Proton and Deuterium Relaxation in Cartilage , 1995, Magnetic resonance in medicine.

[24]  A. Maroudas,et al.  Swelling pressures of proteoglycans at the concentrations found in cartilaginous tissues. , 1979, Biorheology.

[25]  Lee,et al.  Brillouin-scattering study of hyaluronic acid: Dynamic coupling with the water of hydration and phase transitions. , 1993, Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics.