The malarial infection can alter the normal biodistribution of a radiopharmaceutical

Objective: Infection with Plasmodium berghei is a model of murine malaria widely used in experimental studies, similar to Plasmodium falciparum in humans. The aim of this study was to investigate in vivo the influence of P. berghei infection on the biodistribution of sodium pertechnetate (Na99mTcO4) in mice. Methods: 14 Swiss mice were divided into two groups: control (n=7) and treated (n=7). Treated group were inoculated with 1x105 P. berghei infected red blood cells each. On the 15th day, the infected group and the control group received 0.1 mL (0.66 MBq) of Na99mTcO4. After 40 minutes, all animals were killed and kidney, liver, stomach and blood samples were isolated and the percentage of radioactivity per gram of tissue (%ATI/g) was determined. Parts of tissue samples were used for histological analysis. Data were compared by t-Student test and Mann-Whitney test, considering p<0.05 statistically significant. Results: No statistically significant difference in uptake of ATI%/g was observed in any of the organs. Statistically significant histological changes were seen in the liver of the infected group (cell vacuolization and necrosis) and in the kidney (alterations in the parietal cells and mesangial cells). Conclusions: Although the histopathological finding of necrosis has been a significant result, this change did not alter the uptake of technetium-99m at the site of infection. The parasitic infection by acute malaria or the degree of liver injury probably will not affect the performance of nuclear medicine examinations.

[1]  MK Mohapatra,et al.  Delayed gastric emptying time in adult cerebral Falciparum malaria. , 2012, Journal of vector borne diseases.

[2]  K. Mukhopadhyay,et al.  Applications of the Carrier Free Radioisotopes of Second Transition Series Elements in the Field of Nuclear Medicine , 2011 .

[3]  R. Gasser,et al.  Malaria caused by Plasmodium vivax complicated by acalculous cholecystitis. , 2011, The American journal of tropical medicine and hygiene.

[4]  P. Rosenthal,et al.  Functional Evaluation of Plasmodium Export Signals in Plasmodium berghei Suggests Multiple Modes of Protein Export , 2010, PloS one.

[5]  A. C. Medeiros,et al.  Trypanosoma cruzi: biodistribution of technetium-99m pertechnetate in infected rats. , 2009, Experimental parasitology.

[6]  A. C. Medeiros,et al.  Effect of tripanosomicide benznidazole (Rochagan®) on the biodistribution of sodium pertechnetate (Na99mTcO4) in Wistar rats , 2008 .

[7]  D. Tweardy,et al.  Substance P Receptor Antagonism for Treatment of Cryptosporidiosis in Immunosuppressed Mice , 2008, The Journal of parasitology.

[8]  J. Brandão-Neto,et al.  Biodistribution of the radiopharmaceutical sodium pertechnetate after biliopancreatic bypass with a duodenal switch , 2007 .

[9]  Sang-Woo Lee,et al.  Evaluation of hepatic function with 99mTc-galactosylated serum albumin scintigraphy in patients with malaria: Comparison with 99mTc-colloid scintigraphy and liver ultrasonography , 2007, Nuclear medicine communications.

[10]  J. Gookin,et al.  Neutrophils Do Not Mediate the Pathophysiological Sequelae of Cryptosporidium parvum Infection in Neonatal Piglets , 2006, Infection and Immunity.

[11]  M. Bernardo-Filho,et al.  Effect of antimalarial drugs on the bioavailability of the methylenediphosphonic acid labeled with technetium99m (99mTc-MDP) in Wistar rats , 2006 .

[12]  L. C. Brito,et al.  Drug interaction with radiopharmaceuticals: a review , 2005 .

[13]  Luís Cristóvão Porto,et al.  Sodium pertechnetate (Na99mTcO4) biodistribution in mice exposed to cigarette smoke , 2005, BMC nuclear medicine.

[14]  R. Snow,et al.  The burden of the neurocognitive impairment associated with Plasmodium falciparum malaria in sub-saharan Africa. , 2004, The American journal of tropical medicine and hygiene.

[15]  D. Conway,et al.  A large focus of naturally acquired Plasmodium knowlesi infections in human beings , 2004, The Lancet.

[16]  K. Mendis,et al.  Evolutionary and Historical Aspects of the Burden of Malaria , 2002, Clinical Microbiology Reviews.

[17]  F. Braga,et al.  Nuclear medicine in tropical diseases , 2001, European Journal of Nuclear Medicine.

[18]  W. Waters,et al.  Cryptosporidium parvum infection in T-cell receptor (TCR)-alpha- and TCR-delta-deficient mice , 1996, Infection and immunity.

[19]  W. Becker,et al.  The contribution of nuclear medicine to the patient with infection , 1995, European Journal of Nuclear Medicine.

[20]  M. Bernardo-Filho,et al.  Oxamniquine: a labeling procedure with technetium-99m and a biodistribution study in mice. , 1994, Journal of nuclear biology and medicine.

[21]  W. Schulenburg,et al.  Measures of capillary permeability in acute falciparum malaria: relation to severity of infection and treatment. , 1992, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[22]  Madan M. Rehani,et al.  Site of Tc-99m binding to the red blood cell: concise communication. , 1980, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[23]  Gopal B. Saha,et al.  Fundamentals of Nuclear Pharmacy , 1979, Springer New York.

[24]  I. D. de Sá.  [Chloroquine resistance and the search for antimalarial drugs from the 1960s to 1980s]. , 2011, Historia, ciencias, saude--Manguinhos.

[25]  O. Behmer,et al.  Manual de técnicas para histologia: normal e patológica , 2003 .

[26]  B. Ogutu,et al.  Cerebral Malaria , 2003, CNS drugs.

[27]  安達 圭志.  Plasmodium berghei infection in mice induces liver injury by an IL-12- and Toll-like receptor/myeloid differentiation factor 88-dependent mechanism , 2003 .