Purification, identification and preliminary crystallographic studies of an allergenic protein from Solanum melongena.

Solanum melongena (eggplant), a member of the Solanaceae family, is a widely cultivated vegetable crop and is commonly used as a food throughout the world. Allergic reactions caused by members of this family are well known. However, mechanistic analyses to understand their molecular basis have not been adequately explored. In order to address this issue, the 7S vicilin protein (SM80.1) of size 45 kDa was purified from seeds of S. melongena by ammonium sulfate fractionation and size-exclusion chromatography. Significant homology of SM80.1 to an allergy-related protein from S. lycopersicum was identified through a BLAST search. Crystallization attempts with purified protein using the hanging-drop vapour-diffusion method led to hexagonal-shaped crystals. The crystals diffracted to 2.21 Å resolution and belonged to space group P6322, with unit-cell parameters a = 117.9, c = 123.5 Å.

[1]  A. Barone,et al.  Production of Pharmaceutical Proteins in Solanaceae Food Crops , 2013, International journal of molecular sciences.

[2]  S. Selvaraj,et al.  In vitro antioxidant activities of Solanum surattense leaf extract. , 2013, Asian Pacific journal of tropical biomedicine.

[3]  Owen Johnson,et al.  iMOSFLM: a new graphical interface for diffraction-image processing with MOSFLM , 2011, Acta crystallographica. Section D, Biological crystallography.

[4]  Rajnish A. Gupta,et al.  Antidiabetic and antioxidant potential of β‐sitosterol in streptozotocin‐induced experimental hyperglycemia , 2011, Journal of diabetes.

[5]  D. Salunke,et al.  Crystal Structure and Functional Insights of Hemopexin Fold Protein from Grass Pea1[W][OA] , 2010, Plant Physiology.

[6]  A. Ramazani,et al.  In vitro and in vivo anti-malarial activity of Boerhavia elegans and Solanum surattense , 2010, Malaria Journal.

[7]  W. Weckwerth,et al.  Evidence for novel tomato seed allergens: IgE-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling. , 2009, Journal of proteome research.

[8]  Pankaj Gupta,et al.  Purification, identification and preliminary crystallographic studies of a 2S albumin seed protein from Lens culinaris. , 2008, Acta crystallographica. Section F, Structural biology and crystallization communications.

[9]  S. N. Pramod,et al.  Allergy to eggplant (Solanum melongena) caused by a putative secondary metabolite. , 2008, Journal of investigational allergology & clinical immunology.

[10]  Dinakar M Salunke,et al.  Purification, identification and preliminary crystallographic studies of Pru du amandin, an allergenic protein from Prunus dulcis. , 2008, Acta crystallographica. Section F, Structural biology and crystallization communications.

[11]  D. Salunke,et al.  Purification, identification and preliminary crystallographic studies of an allergenic protein from Lathyrus sativus. , 2006, Acta crystallographica. Section F, Structural biology and crystallization communications.

[12]  Xiaojun Liu,et al.  A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress- and pathogen-inducible. , 2006, Journal of plant physiology.

[13]  D. Salunke,et al.  Purification, identification and preliminary crystallographic characterization of a novel seed protein from Vigna unguiculata. , 2004, Acta crystallographica. Section D, Biological crystallography.

[14]  C. Radauer,et al.  A classification of plant food allergens. , 2004, The Journal of allergy and clinical immunology.

[15]  K. Hoffmann‐Sommergruber,et al.  Cloning and Molecular and Immunological Characterisation of Two New Food Allergens, Cap a 2 and Lyc e 1, Profilins from Bell Pepper (Capsicum annuum) and Tomato (Lycopersicon esculentum) , 2003, International Archives of Allergy and Immunology.

[16]  S. Scheurer,et al.  Biological activity of IgE specific for cross-reactive carbohydrate determinants. , 2003, The Journal of allergy and clinical immunology.

[17]  S. Scheurer,et al.  Molecular characterization and allergenic activity of Lyc e 2 (beta-fructofuranosidase), a glycosylated allergen of tomato. , 2003, European journal of biochemistry.

[18]  A. Posch,et al.  Evaluation of patatin as a major cross-reactive allergen in latex-induced potato allergy. , 2002, Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology.

[19]  T. Reunala,et al.  Identification of four novel potato (Solanum tuberosum) allergens belonging to the family of soybean trypsin inhibitors , 2001, Allergy.

[20]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[21]  M. Yamaguchi,et al.  World Vegetables: Principles, Production and Nutritive Values , 1983 .