Fiber-optic detection device for GC-UV

Previous studies of the hyphenation of gas chromatographic separation and spectrophotometric detection in the ultraviolet wavelength range between 168 and 330 nm showed a high potential for applications where the analysis of complex samples is required. Within this paper the development of a state-of-the-art detection system for compounds in the vapour phase is described, offering an improved behaviour compared to previous systems: Dependent on the requirements of established detection systems hyphenated with gas chromatography, the main components of the system have to be designed for optimum performance and reliability of the spectrophotometric detector: A deuterium lamp as a broadband light source has been selected for improved stability in the measurements. A new-type absorption cell based on fiber-optics has been developed considering the dynamic necessary to compete with existing techniques. In addition, the influence of the volume of the cell on the chromatogram needs to be analyzed. Tests for determining the performance of the absorption cell in terms of chemical and thermal influences have been carried out. A new spectrophotometer with adequate spectral resolution in the wavelength range, offering improved stability and dynamic for an efficient use in this application was developed. Furthermore, the influence of each component on the performance, reliability and stability of the sensor system will be discussed. An overview and outlook over the potential applications in the environmental, scientific and medical field will be given.

[1]  J Andrasko,et al.  Identification of compounds and specific functional groups in the wavelength region 168-330 nm using gas chromatography with UV detection. , 2000, Journal of chromatography. A.

[2]  H. Jaffe,et al.  Theory and Applications of Ultraviolet Spectroscopy , 1962 .

[3]  Karl-Friedrich Klein,et al.  New analytical applications with UV fiber bundles , 2001, SPIE BiOS.

[4]  Anders Nilsson,et al.  Novel Technique for Analysing Volatile Compounds in Indoor Dust : Application of Gas Chromatography – UV Spectrometry to the Study of Building-Related Illness , 2004 .

[5]  T Cedrón-Fernández,et al.  Separation and determination of volatile compounds in synthetic wine samples by gas chromatography using UV-visible molecular absorption spectrometry as detector. , 2002, Talanta.

[6]  David Smith,et al.  Time variation of ammonia, acetone, isoprene and ethanol in breath: a quantitative SIFT-MS study over 30 days. , 2003, Physiological measurement.

[7]  Recommendations for Standardized Procedures for the Online and Offline Measurement of Exhaled Lower Respiratory Nitric Oxide and Nasal Nitric Oxide in Adults and Children — 1999 , 1999 .

[8]  Kenneth T. V. Grattan,et al.  Further improvements of high-precision gas analysis in DUV region , 2004, SPIE BiOS.

[9]  Sune Svanberg,et al.  Atomic and Molecular Spectroscopy , 2022, Graduate Texts in Physics.

[10]  Mitsunobu Miyagi,et al.  Hollow fiber delivery of ArF and KrF excimer laser light , 1999, Photonics West - Biomedical Optics.

[11]  W. Kaye,et al.  Far-Ultraviolet Spectroscopic Detection of Gas Chromatograph Effluent. , 1962 .

[12]  C. N. Banwell,et al.  Fundamentals of molecular spectroscopy , 1966 .

[13]  T. Sun,et al.  Fiber-optic based gas sensing in the UV region , 2006, SPIE BiOS.

[14]  C Tagesson,et al.  Determination of isoprene in human breath by thermal desorption gas chromatography with ultraviolet detection. , 1995, Journal of chromatography. B, Biomedical applications.

[15]  Verner Lagesson.  Micro gas chromatographic separation combined with UV- and IR-spectrophotometric detection/identification with applications within the occupational hygiene field using diffusive and active sampling followed by a direct thermal desorption technique , 1992 .