Streaming potential of human lumbar anulus fibrosus is anisotropic and affected by disc degeneration.

The streaming potential responses of non-degenerate and degenerate human anulus fibrosus were measured in a one-dimensional permeation configuration under static and dynamic loading conditions. The goal of this study was to investigate the influence of the changes in tissue structure and composition on the electrokinetic behavior of intervertebral disc tissues. It was found that the static streaming potential of the anulus fibrosus depended on the degenerative grade of the discs (p = 0.0001) and on the specimen orientation in which the fluid flows (p = 0.0001). For a statically applied pressure of 0.07 MPa, the ratio of streaming potential to applied pressure ranged from 5.3 to 6.9 mV/MPa and was largest for Grade I tissue with axial orientation and lowest for Grade III tissue with circumferential orientation. The dynamic streaming potential responses of anulus fibrosus were sensitive to the degeneration of the disc: the total harmonic distortion factor increased by 108%, from 3.92 +/- 0.66% (mean +/- SD) for Grade I specimens to 8.15 +/- 3.05% for Grades II and III specimens. The alteration of streaming potential reflects the changes in tissue composition and structure with degeneration. To our knowledge, this is the first reported data for the streaming potential of human intervertebral disc tissues. Knowledge of the streaming potential response of the intervertebral disc provides an understanding of potentially important signal transduction mechanisms in the disc and of the etiology of intervertebral disc degeneration.

[1]  F. Marchand,et al.  Investigation of the Laminate Structure of Lumbar Disc Anulus Fibrosus , 1990, Spine.

[2]  A. Maroudas,et al.  Swelling of the intervertebral disc in vitro. , 1981, Connective tissue research.

[3]  J. Galante Tensile properties of the human lumbar annulus fibrosus. , 1967, Acta orthopaedica Scandinavica.

[4]  A. Grodzinsky,et al.  Cartilage electromechanics--I. Electrokinetic transduction and the effects of electrolyte pH and ionic strength. , 1987, Journal of biomechanics.

[5]  A Ratcliffe,et al.  Compressive mechanical properties of the human anulus fibrosus and their relationship to biochemical composition. , 1994, Spine.

[6]  Richard H. Rothman,et al.  The intervertebral disc , 1970 .

[7]  J. Urban,et al.  Swelling Pressure of the Lumbar Intervertebral Discs: Influence of Age, Spinal Level, Composition, and Degeneration , 1988, Spine.

[8]  Transport of Multi-Electrolytes in Charged Hydrated Biological Soft Tissues , 1999 .

[9]  D. Roylance,et al.  Oscillatory compressional behavior of articular cartilage and its associated electromechanical properties. , 1981, Journal of biomechanical engineering.

[10]  J. P. Thompson,et al.  Preliminary Evaluation of a Scheme for Grading the Gross Morphology of the Human Intervertebral Disc , 1990, Spine.

[11]  D. S. Hickey,et al.  Relation Between the Structure of the Annulus Fibrosus and the Function and Failure of the Intervertebral Disc , 1980, Spine.

[12]  V C Mow,et al.  The anisotropic hydraulic permeability of human lumbar anulus fibrosus. Influence of age, degeneration, direction, and water content. , 1999, Spine.

[13]  G B Andersson,et al.  A model to study the disc degeneration process. , 1994, Spine.

[14]  Van C. Mow,et al.  Degeneration and Aging Affect the Tensile Behavior of Human Lumbar Anulus Fibrosus , 1995, Spine.

[15]  M. Glimcher,et al.  Electromechanical properties of articular cartilage during compression and stress relaxation , 1978, Nature.

[16]  J Black,et al.  Electromechanical properties in human articular cartilage. , 1974, The Journal of bone and joint surgery. American volume.

[17]  J. Buckwalter,et al.  Musculoskeletal soft-tissue aging : impact on mobility , 1993 .

[18]  A. Grodzinsky,et al.  Cartilage electromechanics--II. A continuum model of cartilage electrokinetics and correlation with experiments. , 1987, Journal of biomechanics.

[19]  A. Maroudas,et al.  Physicochemical properties of cartilage in the light of ion exchange theory. , 1968, Biophysical journal.

[20]  A. Grodzinsky,et al.  Streaming potentials: A sensitive index of enzymatic degradation in articular cartilage , 1987, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[21]  W M Lai,et al.  A triphasic theory for the swelling and deformation behaviors of articular cartilage. , 1991, Journal of biomechanical engineering.

[22]  A. Maroudas,et al.  The correlation of fixed negative charge with glycosaminoglycan content of human articular cartilage. , 1969, Biochimica et biophysica acta.

[23]  W M Lai,et al.  A mixture theory for charged-hydrated soft tissues containing multi-electrolytes: passive transport and swelling behaviors. , 1998, Journal of biomechanical engineering.

[24]  W M Lai,et al.  Transport of fluid and ions through a porous-permeable charged-hydrated tissue, and streaming potential data on normal bovine articular cartilage. , 1993, Journal of biomechanics.

[25]  R. J. Pawluk,et al.  Electrical Behavior of Cartilage during Loading , 1972, Science.

[26]  J. Urban,et al.  Swelling pressure of the inervertebral disc: influence of proteoglycan and collagen contents. , 1985, Biorheology.