Transitory expression of Thy-1 antigen in immature B cell lines.

In cmu- B220+ Thy-1- murine immature B cells transformed with a temperature-sensitive mutant of Abelson murine leukemia virus, a low level of Thy-1 antigen was transitorily expressed after the shift of the culture temperature from the permissive (35 degrees C) to the non-permissive (39 degrees C) temperature. On the other hand, B220 antigen was persistently expressed regardless of whether the cells were cultured at the permissive or non-permissive temperature. No other T-lineage-specific antigens, CD3, CD4, and CD8 were induced during the culture at the non-permissive temperature. Expression of Thy-1 antigen was confirmed by the detection of a low level of Thy-1-specific mRNA. These results clearly showed that immature B cells could express Thy-1 antigen during proliferation and/or differentiation. The results imply a role for Thy-1 antigen in B cell proliferation and/or differentiation.

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