Amperometric biosensor for lactate analysis in wine and must during fermentation

Abstract A lactate oxidase-based amperometric biosensor is designed for lactate determination. Two methods of lactate oxidase immobilization on the surface of commercial SensLab platinum printing electrodes are compared. The sensor with lactate oxidase immobilized by physical adsorption in Resydrol polymer is shown to have both narrower dynamic range (0.004–0.5 mМ lactate) and higher sensitivity (320 nA/mM) as compared with that immobilized in poly(3,4-ethylenedioxythiophene) by electrochemical polymerization (0.05–1.6 mM and 60 nA/mM respectively). The operational stability of the biosensors developed is studied; the immobilization method is shown to be of no influence. The lactate content in wine and in wine material during fermentation is analyzed. The data obtained by amperometric lactate biosensor correlated with those of standard chromatography. The biosensor developed can be used in food industry for control and optimization of process of wine fermentation as well as for control of wine quality.

[1]  Bansi D. Malhotra,et al.  Co-immobilization of lactate oxidase and lactate dehydrogenase on conducting polyaniline films , 2000 .

[2]  B. Piro,et al.  Electrochemical method for entrapment of oligonucleotides in polymer-coated electrodes. , 1999, Journal of biomedical materials research.

[3]  G. Jobst,et al.  Biosensor arrays for simultaneous measurement of glucose, lactate, glutamate, and glutamine. , 2002, Biosensors & bioelectronics.

[4]  M. Comtat,et al.  Simultaneous use of dehydrogenases and hexacyanoferrate(III) ion in electrochemical biosensors for l-lactate, d-lactate and l-glutamate ions , 1993 .

[5]  C. Kurzawa,et al.  Immobilization method for the preparation of biosensors based on pH shift-induced deposition of biomolecule-containing polymer films. , 2002, Analytical chemistry.

[6]  D. Centonze,et al.  Simultaneous monitoring of glucose and lactate by an interference and cross-talk free dual electrode amperometric biosensor based on electropolymerized thin films. , 2000, Biosensors & bioelectronics.

[7]  J H Luong,et al.  Developments and applications of biosensors in food analysis. , 1997, Trends in biotechnology.

[8]  A. O. Rangel,et al.  Enzymatic determination of L(+) lactic and L(-) malic acids in wines by flow-injection spectrophotometry , 1998 .

[9]  Serge Lefrant,et al.  In situ spectroelectrochemical Raman studies of poly(3,4-ethylenedioxythiophene) (PEDT) , 1999 .

[10]  S. Dzyadevych,et al.  Development of amperometric enzyme biosensor based on carbon fibre electrode and immobilized glucose oxidase , 2003 .

[11]  Jean-Louis Marty,et al.  Screen-printed biosensors for the control of wine quality based on lactate and acetaldehyde determination , 2002 .

[12]  P. Coulet,et al.  A reliable l-lactate electrode with a new membrane for enzyme immobilization for amperometric assay of lactate , 1986 .

[13]  H. Ukeda,et al.  Simultaneous determination of glucose, ethanol and lactate in alcoholic beverages and serum by amperometric flow injection analysis with immobilized enzyme reactors. , 1990, Journal of biotechnology.

[14]  J. Pingarrón,et al.  Graphite-Teflon composite bienzyme electrodes for the determination of L-lactate: application to food samples. , 1999, Biosensors & bioelectronics.

[15]  M. Comtat,et al.  Bienzyme amperometric lactate-specific electrode , 1990 .

[16]  S. Armes,et al.  Synthesis and Characterization of Micrometer-Sized Poly(3,4-ethylenedioxythiophene)-Coated Polystyrene Latexes , 1999 .

[17]  Danila Moscone,et al.  Electrochemical biosensors for monitoring malolactic fermentation in red wine using two strains of Oenococcus oeni , 2004 .

[18]  G. Palleschi,et al.  Oxidase enzyme immobilisation through electropolymerised films to assemble biosensors for batch and flow injection analysis. , 2003, Biosensors & bioelectronics.

[19]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[20]  J. Korf,et al.  On-line continuous monitoring of glucose or lactate by ultraslow microdialysis combined with a flow-through nanoliter biosensor based on poly(m-phenylenediamine) ultra-thin polymer membrane as enzyme electrode. , 2001, The Analyst.

[21]  F. Jonas,et al.  Poly(alkylenedioxythiophene)s—new, very stable conducting polymers , 1992 .

[22]  Jean-Louis Marty,et al.  Chronoamperometric determination of d-lactate using screen-printed enzyme electrodes , 2001 .

[23]  J. Švitel,et al.  Amperometric biosensors based on solid binding matrices applied in food quality monitoring. , 1998, Biosensors & bioelectronics.

[24]  J H Luong,et al.  The potential role of biosensors in the food and drink industries. , 1991, Biosensors & bioelectronics.