Comparison of Four Methods to Estimate the Dissolved Nitrogen Fraction in Range Plants

Aboveground biomass of four range species was collected at several phenological stagesand total nitrogen wasdetermined. The dissolvable nitrogen fraction within these samples was estimated utilizing four techniques: (1) in vivo nylon bag digestion in a rumen-flstulated Bison bison; (2) in vitro Tllley and Terry plus pepsin; (3) neutral detergent fiber; and (4) laboratory detergent fiber. Total nitrogen concentration in all plant species studied was highest during early growth and decreased with advancing maturity. A similar amount of nitrogen was removed from dead or dormant plant materials using any technique, but significantly more nitrogen was removed from green succulent material utilizing the nylon bag technique than was removed with the three laboratory assays. The amount of nitrogen removed from plant foliage was highly correlated among techniques. Equations were developed to predict nitrogen losses with the nylon bag technique using dissolved nitrogen values obtained from any of the other three techniques.

[1]  Richard Mark Hansen,et al.  A comparison of esophageal fistula and fecal material to determine steer diets. , 1978 .

[2]  Richard Mark Hansen,et al.  Blacktail prairie dogs, desert cottontails and cattle trophic relations on shortgrass range. , 1977 .

[3]  P. J. Urness,et al.  Comparison of in vivo and in vitro dry matter digestibility of mule deer forages. , 1977 .

[4]  W. Toma,et al.  Microdigestion Techniques and Chemical Solubility Methods as Estimators of the Digestibility of Tropical Grasses 1 , 1976 .

[5]  D. Peden,et al.  The Trophic Ecology of Bison bison L. on Shortgrass Plains , 1974 .

[6]  J. D. Wallace,et al.  Seasonal changes in herbage and cattle diets on sandhill grassland. , 1972 .

[7]  T. Bedell.  Nutritive value of forage and diets of sheep and cattle from Oregon subclover-grass mixtures. , 1971 .

[8]  É. Grenet,et al.  The use of the nylon bag technique for the study of forage digestion in the rumen and for predicting feed value. , 1970 .

[9]  H. Pearson.  Digestibility trials: in vitro techniques. , 1970 .

[10]  P. V. Soest,et al.  Use of Detergents in the Analysis of Fibrous Feeds. IV. Determination of Plant Cell-Wall Constituents , 1967 .

[11]  Van Soest.  Nonnutritive Residues: A System of Analysis for the Replacement of Crude Fiber , 1966 .

[12]  D. Cable,et al.  Crude Protein in Rumen Contents and in Forage. , 1966 .

[13]  J. M. A. Tilley,et al.  A TWO-STAGE TECHNIQUE FOR THE IN VITRO DIGESTION OF FORAGE CROPS , 1963 .

[14]  G. M. V. Dyne.  Micro-Methods for Nutritive Evaluation Of Range Forages. , 1962 .

[15]  B. Taylor.  An artificial rumen technique versus conventional digestion trials for determining digestibility of blue grama, Sudan and alfalfa hays. , 1960 .