Discovery of Novel Materials with Broad Resistance to Bacterial Attachment Using Combinatorial Polymer Microarrays

A new class of bacteria-attachment-resistant materials is discovered using a multi-generation polymer microarray methodology that reduces bacterial attachment by up to 99.3% compared with a leading commercially available silver hydrogel anti-bacterial material. The coverage of three bacterial species, Pseudomonas aeruginosa, Staphylococcus aureus, and uropathogenic Escherichia coli is assessed.

[1]  S. Diggle,et al.  Inhibition and dispersion of Pseudomonas aeruginosa biofilms by glycopeptide dendrimers targeting the fucose-specific lectin LecB. , 2008, Chemistry & biology.

[2]  M. Alexander,et al.  TOF-SIMS analysis of a 576 micropatterned copolymer array to reveal surface moieties that control wettability. , 2008, Analytical chemistry.

[3]  Daniel G. Anderson,et al.  Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells , 2004, Nature Biotechnology.

[4]  Mark Bradley,et al.  Colonising new frontiers—microarrays reveal biofilm modulating polymers , 2011 .

[5]  Ying Mei,et al.  Combinatorial Development of Biomaterials for Clonal Growth of Human Pluripotent Stem Cells , 2010, Nature materials.

[6]  O. Wenker,et al.  A comparison of two antimicrobial-impregnated central venous catheters. Catheter Study Group. , 1999, The New England journal of medicine.

[7]  Axel Rosenhahn,et al.  The role of "inert" surface chemistry in marine biofouling prevention. , 2010, Physical chemistry chemical physics : PCCP.

[8]  Henny C van der Mei,et al.  Inhibition of adhesion of yeasts and bacteria by poly(ethylene oxide)-brushes on glass in a parallel plate flow chamber. , 2003, Microbiology.

[9]  S. Eykyn.  Microbiology , 1950, The Lancet.

[10]  K. Winzer,et al.  Look who's talking: communication and quorum sensing in the bacterial world , 2007, Philosophical Transactions of the Royal Society B: Biological Sciences.

[11]  Robert Langer,et al.  High Throughput Surface Characterisation of a Combinatorial Material Library , 2007 .

[12]  R. Misra,et al.  Biomaterials , 2008 .

[13]  Anthony W Smith,et al.  Biofilms and antibiotic therapy: is there a role for combating bacterial resistance by the use of novel drug delivery systems? , 2005, Advanced drug delivery reviews.

[14]  Elazer R. Edelman,et al.  Adv. Drug Delivery Rev. , 1997 .

[15]  F. Guittard,et al.  Synthesis and antimicrobial properties of polymerizable quaternary ammoniums. , 2009, European journal of medicinal chemistry.

[16]  James D Bryers,et al.  Zwitterionic carboxybetaine polymer surfaces and their resistance to long-term biofilm formation. , 2009, Biomaterials.

[17]  Robert Langer,et al.  Polymer surface functionalities that control human embryoid body cell adhesion revealed by high throughput surface characterization of combinatorial material microarrays. , 2010, Biomaterials.

[18]  L. Sampath,et al.  Decreased bacterial adherence and biofilm formation on chlorhexidine and silver sulfadiazine-impregnated central venous catheters implanted in swine. , 1995, Critical care medicine.

[19]  S. Diggle,et al.  The galactophilic lectin, LecA, contributes to biofilm development in Pseudomonas aeruginosa. , 2006, Environmental microbiology.

[20]  J. Costerton,et al.  Bacterial biofilms: a common cause of persistent infections. , 1999, Science.

[21]  K. Mellits,et al.  Development of gfp vectors for expression in Listeria monocytogenes and other low G+C gram positive bacteria , 2001, Microbial Ecology.

[22]  Ashutosh Chilkoti,et al.  In Pursuit of Zero: Polymer Brushes that Resist the Adsorption of Proteins , 2009 .

[23]  Robert Langer,et al.  Combinatorial discovery of polymers resistant to bacterial attachment , 2012, Nature Biotechnology.

[24]  Andrew L. Hook,et al.  High throughput discovery of thermo-responsive materials using water contact angle measurements and time-of-flight secondary ion mass spectrometry , 2012, Surface and interface analysis : SIA.

[25]  C. Edmiston,et al.  Activity of antibacterial impregnated central venous catheters against Klebsiella pneumoniae , 2002, Intensive Care Medicine.

[26]  Mischa Zelzer,et al.  Picoliter water contact angle measurement on polymers. , 2007, Langmuir : the ACS journal of surfaces and colloids.