In vivo Effects of a Recombinant Molt-Inhibiting Hormone on Molt Interval and Hemolymph Ecdysteroid Level in the Kuruma Prawn, Marsupenaeus japonicus

Abstract In order to determine the function of molt-inhibiting hormone (MIH) in vivo, we examined the effects of injecting of a recombinant MIH on the molt interval and hemolymph ecdysteroid level in the kuruma prawn, Marsupenaeus japonicus. The injection of recombinant MIH significantly prolonged the molt interval (9.0±0.4 days in the control group, 9.5±0.5 days in the 2500 ng/g-body weight/injection-group, mean±SD), and significantly decreased the hemolymph ecdysteroid level (ratio of levels between after and before injection: 1.94±1.09 in the control and 1.28±0.39 in the 3000 ng/g-body weight/injection-group, mean±SD). These results conclusively show the inhibitory effects of MIH on molting in vivo.

[1]  R. Keller,et al.  Crustacean neuropeptides: Structures, functions and comparative aspects , 1992, Experientia.

[2]  T. Nakatsuji,et al.  Regulation of ecdysteroid secretion from the Y-organ by molt-inhibiting hormone in the American crayfish, Procambarus clarkii. , 2004, General and comparative endocrinology.

[3]  T. Nakatsuji,et al.  Measurement of Molt-inhibiting Hormone Titer in Hemolymph of the American Crayfish, Procambarus clarkii, by Time-Resolved Fluoroimmunoassay , 2003, Zoological science.

[4]  J. Chung,et al.  Moult cycle-related changes in biological activity of moult-inhibiting hormone (MIH) and crustacean hyperglycaemic hormone (CHH) in the crab, Carcinus maenas. From target to transcript. , 2003, European journal of biochemistry.

[5]  K. Aida,et al.  Effects of bilateral eyestalk ablation on molting and ovarian development in the giant freshwater prawn, Macrobrachium rosenbergii , 2001 .

[6]  T. Kawakami,et al.  Synthesis of a molt-inhibiting hormone of the American crayfish, Procambarus clarkii, and determination of the location of its disulfide linkages. , 2000, Journal of biochemistry.

[7]  H. Nagasawa,et al.  Expression of a recombinant molt-inhibiting hormone of the kuruma prawn Penaeus japonicus in Escherichia coli. , 1999, Bioscience, biotechnology, and biochemistry.

[8]  G. Coast,et al.  Recent advances in arthropod endocrinology , 1999 .

[9]  G. Coast,et al.  Ecdysteroid synthesis in the crustacean Y-organ: role of cyclic nucleotides and Ca2+. , 1998 .

[10]  H. Nagasawa,et al.  Amino acid sequence of a peptide with molt-inhibiting activity from the kuruma prawn Penaeus japonicus , 1996, Peptides.

[11]  J. L. Le Caer,et al.  Characterization of hyperglycemic and molt-inhibiting activity from sinus glands of the penaeid shrimp Penaeus vannamei. , 1996, General and comparative endocrinology.

[12]  D. D. de Kleijn,et al.  Molecular biology of neurohormone precursors in the eyestalk of Crustacea. , 1995, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[13]  Yuzuru Suzuki,et al.  Changes in Hemolymph Vitellogenin and Ecdysteroid Levels during the Reproductive and Non-Reproductive Molt Cycles in the Freshwater Prawn Macrobrachium nipponense , 1992 .

[14]  E. S. Chang,et al.  Ecdysteroids in relation to the molt cycle of the American lobster, Homarus americanus. I. Hemolymph titers and metabolites. , 1991, General and comparative endocrinology.

[15]  K. Aida,et al.  Hemolymph ecdysteroid levels during the molt cycle in the Kuruma prawn Penaeus japonicus , 1989 .

[16]  J. Grassi,et al.  Development of an enzyme immunoassay for ecdysteroids using acetylcholinesterase as label , 1989 .

[17]  M. Mattson.  New insights into neuroendocrine regulation of the crustacean molt cycle , 1986 .

[18]  E. S. Chang,et al.  EFFECTS OF SINUS GLAND EXTRACTS ON LARVAL MOLTING AND ECDYSTEROID TITERS OF THE AMERICAN LOBSTER, HOMARUS AMERICANUS , 1986 .

[19]  D. M. Skinner.  2 – Molting and Regeneration , 1985 .

[20]  E. S. Chang,et al.  Demonstration of a molt-inhibiting hormone from the sinus gland of the lobster, Homaru americanus , 1984 .