Distinguishing the viability of a single yeast cell with an ultra-sensitive radio frequency sensor.

We propose and demonstrate a simple, ultra sensitive radio frequency (RF) sensor to detect a single yeast cell and distinguish its viability in a microfluidic channel. On-chip interference is used to cancel background probing signals to improve sensor sensitivity. Individual viable and nonviable yeast cells (approximately 5.83 +/- 0.85 microm in diameter) are measured with clear sensing and identification of these cells.

[1]  Alexandra Ros,et al.  Microfluidic single-cell analysis of intracellular compounds , 2008, Journal of The Royal Society Interface.

[2]  Koji Asami,et al.  Dielectric spectroscopy of biological cells , 1996 .

[3]  P. H. Yap,et al.  Refractive index measurement of single living cells using on-chip Fabry-Pérot cavity , 2006 .

[4]  Urban Seger,et al.  Dielectric spectroscopy in a micromachined flow cytometer: theoretical and practical considerations. , 2004, Lab on a chip.

[5]  D K Wood,et al.  A feasible approach to all-electronic digital labeling and readout for cell identification. , 2007, Lab on a chip.

[6]  K Asami,et al.  Dielectric behavior of wild-type yeast and vacuole-deficient mutant over a frequency range of 10 kHz to 10 GHz. , 1996, Biophysical journal.

[7]  Christian H. Reccius,et al.  Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry. , 2009, Lab on a chip.

[8]  Roy Clarke,et al.  Single bacterial cell detection with nonlinear rotational frequency shifts of driven magnetic microspheres , 2007 .

[9]  S. Gawad,et al.  Micromachined impedance spectroscopy flow cytometer for cell analysis and particle sizing. , 2001, Lab on a chip.

[10]  A. Manz,et al.  Micro total analysis systems. 1. Introduction, theory, and technology. , 2002, Analytical chemistry.

[11]  Kai Chang,et al.  Uniplanar power dividers using coupled CPW and asymmetrical CPS for MICs and MMICs , 1996 .

[12]  Howard M. Shapiro,et al.  Practical Flow Cytometry , 1985 .

[13]  Guoqing Hu,et al.  DC dielectrophoretic focusing of particles in a serpentine microchannel , 2009 .

[14]  Andrew Cleland,et al.  High-bandwidth radio frequency Coulter counter , 2005 .

[15]  Chunrong Song,et al.  A radio frequency device for measurement of minute dielectric property changes in microfluidic channels , 2009 .

[16]  C. Lo,et al.  Impedance analysis of MDCK cells measured by electric cell-substrate impedance sensing. , 1995, Biophysical journal.

[17]  Vasan Venugopalan,et al.  Characterization and use of laser-based lysis for cell analysis on-chip , 2008, Journal of The Royal Society Interface.