Variations in random amplified polymorphic DNA patterns and secondary metabolite profiles withinFusariumspecies from cereals from various parts of The Netherlands

Abstract Random amplified polymorphic DNA pattern (RAPD pattern) analysis and secondary metabolite profile (SM profile) assays were used to investigate genetic variability within Fusarium culmorum and Fusarium avenaceum isolated from four geographic regions in The Netherlands. The main aim was to investigate the genotypic and phenotypic variability within the two species in relation to geographic origin. The occurrence of isolates with a certain characteristic set of RAPD pattern and SM profile could have predictive values on the mycotoxins found in the cereals originating from certain geographic regions. Among the F. culmorum isolates, small variations were found with RAPD pattern analysis. F. culmorum isolates belonging to the RAPD type I were found in all four geographic regions in The Netherlands while isolates with RAPD types Ia and Ib were obtained from region B only. All F. culmorum isolates showed a different SM profile. F. avenaceum isolates were very heterogeneous both with RAPD pattern analysis and SM profile assay, resulting in individual identification of each isolate. It is concluded that the combination of RAPD pattern analysis and SM profile assays is very powerful to differentiate Fusarium isolates, permitting visualization of variations between occurring isolates. However, due to high discrimination of both assays, the application for epidemiological procedures seems to be limited.

[1]  M. Nijs,et al.  Isolation of Fusarium DNA for molecular analysis with and without mechanical cell disruption , 1996 .

[2]  F. Rombouts,et al.  Fungal Infection and Presence of Deoxynivalenol and Zearalenone in Cereals Grown in The Netherlands. , 1996, Journal of food protection.

[3]  R. Plattner,et al.  Fumonisin production and other traits of Fusarium moniliforme strains from maize in northeast Mexico , 1994, Applied and environmental microbiology.

[4]  P. Bridge,et al.  An assessment of aesculin hydrolysis, vegetative compatibility and DNA polymorphism as criteria for characterizing pathogenic races within Fusarium oxysporum f.sp. vasinfectum , 1993 .

[5]  F. Muehlbauer,et al.  Use of random amplified polymorphic DNA (RAPD) to characterize race 2 of Fusarium oxysporum f. sp. pisi , 1993 .

[6]  K. Seifert,et al.  Genetic characterization of Fusarium graminearum strains using RAPD and PCR amplification , 1993 .

[7]  F. Rohlf,et al.  NTSYS-pc Numerical Taxonomy and Multivariate Analysis System, version 2.1: Owner manual , 1992 .

[8]  K. Livak,et al.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. , 1990, Nucleic acids research.

[9]  U. Thrane.  Grouping Fusarium section Discolor isolates by statistical analysis of quantitative high performance liquid chromatographic data on secondary metabolite production , 1990 .

[10]  J. Frisvad,et al.  Standardized high-performance liquid chromatography of 182 mycotoxins and other fungal metabolites based on alkylphenone retention indices and UV—VIS spectra (diodearray detection) , 1987 .

[11]  J. Frisvad,et al.  Classification of terverticillate penicillia based on profiles of mycotoxins and other secondary metabolites , 1983, Applied and environmental microbiology.

[12]  P. E. Nelson,et al.  Fusarium species: an illustrated manual for identification. , 1983 .

[13]  Robert A. Samson,et al.  Introduction to food-borne fungi. , 1989 .

[14]  R. Cook.  Gibberella avenacea sp.n., perfect stage of Fusarium roseum f.sp. cerealis `Avenaceum'. , 1967 .