Nanopore development at Oxford Nanopore

VOLUME 34 NUMBER 8 AUGUST 2016 NATURE BIOTECHNOLOGY (Supplementary Fig. 9). Consistently, no substantial off-target effects were detected at potential off-target sites with 2to 4-bp mismatches with the two target sequences in Prkdc (Supplementary Fig. 10). These results suggest that both AsCpf1–crRNA complexes and LbCpf1–crRNA complexes may exhibit minimal off-target effects comparable to results with the Cas9–short guide RNA (sgRNA) complex. Such off-target effects could be avoided by carefully selecting target sequences.