Characterization of size-dependent exchange of PEG molecules between the blood and extravascular space in the pig.

Different sized polyethylene glycols (PEGs) have been used as probe molecules in studies of size-dependent permeation through the intestinal wall and the glomerular membranes. We have curve-fitted a three-compartment model to the urinary recovery data following intravenous injection of different sized PEG molecules in the pig. The rate constants to and from the extravascular space demonstrate a strong size-dependent selectivity for PEG molecules less than 502 Da, but the rate constants to the urine are almost the same for all PEG molecules. This is discussed in relation to the selectivity in the glomerular filtration, distribution volume, and the use of PEG molecules as tracers in the permeability studies.

[1]  C. P. Carpenter,et al.  Renal excretion and volume distribution of some polyethylene glycols in the dog. , 1947, The American journal of physiology.

[2]  K. Fälth‐magnusson,et al.  Intestinal permeability in healthy and allergic children before and after sodium‐cromoglycate treatment assessed with different‐sized polyethyleneglycols (PEG 400 and PEG 1000) , 1984, Clinical allergy.

[3]  R. Sjödahl,et al.  Passage of molecules through the wall of the gastrointestinal tract. Measurement of intestinal permeability to polyethylene glycols in the 634-1338 dalton range (PEG 1000). , 1983, Scandinavian journal of gastroenterology.

[4]  F. Berglund REABSORPTION OF INORGANIC SULFATE BY THE RENAL TUBULES OF THE RAT. , 1964, Acta physiologica Scandinavica.

[5]  D. Marquardt An Algorithm for Least-Squares Estimation of Nonlinear Parameters , 1963 .

[6]  J. Møller,et al.  The glomerular filterability of insulin and of different molecular weight preparations of polyethylene glycol in the rabbit. , 1972, Acta physiologica Scandinavica.

[7]  A. Hofmann,et al.  Measurements of intestinal permeability using low molecular weight polyethylene glycols (PEG 400). II. Application to normal and abnormal permeability states in man and animals. , 1977, Gastroenterology.

[8]  S. Ukabam,et al.  Small intestinal permeability to mannitol, lactulose, and polyethylene glycol 400 in celiac disease , 1984, Digestive diseases and sciences.

[9]  F. Berglund Renal clearances of inulin, polyfructosan-S and a polyethylene glycol (PEG 1,000) in the rat. , 1965, Acta physiologica Scandinavica.

[10]  L. Sköldstam,et al.  Influence of fasting on intestinal permeability and disease activity in patients with rheumatoid arthritis. , 1982, Scandinavian journal of rheumatology.

[11]  E. Seidman,et al.  Increased permeability to polyethylene glycol 4000 in rabbits with experimental colitis. , 1986, Gastroenterology.

[12]  R. Sjödahl,et al.  Passage of molecules through the wall of the gastrointestinal tract. Urinary recovery of different-sized polyethylene glycols after intravenous and intestinal deposition. , 1984, Scandinavian journal of gastroenterology.

[13]  K. Magnusson,et al.  Passage of molecules through the wall of the gastrointestinal tract. II. Application of low-molecular weight polyethyleneglycol and a deterministic mathematical model for determining intestinal permeability in man. , 1980, Gut.