Motive force of cytoplasmic streaming during plasmodial mitosis of physarum polycephalum

The relationship between mitosis and cytoplasmic streaming in the plasmodium of Physarum polycephalum was investigated by simultaneously conducting the following three experiments: (1) identification of the mitotic stages under phase contrast optics, (2) measurement of the rate of oriented migration of the plasmodium on an agar ribbon, and (3) measurement of the motive force of cytoplasmic streaming by the double-chamber method of Kamiya. The migration of the plasmodium almost stopped during synchronous mitosis and the motive force of the flow decreased to 1/4 of the normal level in this period. Gelation of the endoplasm did not occur during the mitotic period, and thus the cessation of the plasmodial migration must have been caused by the diminution of the motive force responsible for cytoplasmic streaming.

[1]  H. Hinssen.  An actin-modulating protein from Physarum polycephalum. II. Ca++-dependence and other properties. , 1981, European journal of cell biology.

[2]  W. Camp,et al.  A Method of Cultivating Myxomycete Plasmodia , 1936 .

[3]  E. Goodman,et al.  Physarum polycephalum: A Review of a Model System Using a Structure-Function Approach , 1980 .

[4]  I. Bernstein,et al.  Adenosine Triphosphate and Synchronous Mitosis in Physarum polycephalum , 1968, Journal of bacteriology.

[5]  S. Takahashi,et al.  Fragmin: a calcium ion sensitive regulatory factor on the formation of actin filaments. , 1980, Biochemistry.

[6]  A. C. Burton,et al.  The relation of cycling of intracellular pH to mitosis in the acellular slime mould Physarum polycephalum , 1977, Journal of cellular physiology.

[7]  順子 岡田,et al.  変形菌 Physarum polycephalum の子実体形成 , 1963 .

[8]  R. Holmes,et al.  Calcium uptake during mitosis in the myxomycete Physarum polycephalum , 1977, Nature.

[9]  N. Kamiya,et al.  THE CONTROL OF PROTOPLASMIC STREAMING. , 1940, Science.

[10]  H. P. Rusch,et al.  Morphological observations on growth and differentation of Physarum polycephalum grown in pure culture. , 1961, Developmental biology.

[11]  F. Matsumura,et al.  Simultaneous oscillations of Ca2+ efflux and tension generation in the permealized plasmodial strand of Physarum. , 1981, Cell motility.

[12]  F. Howard.  Nuclear Division in Plasmodia of Physarum , 1932 .

[13]  N. Kamiya,et al.  Physical and Chemical Basis of Cytoplasmic Streaming , 1981 .

[14]  K Götz von Olenhusen,et al.  Oscillating contractions in protoplasmic strands of Physarum: effects of external Ca++-depletion and Ca++-antagonistic drugs on intrinsic contraction automaticity. , 1977, Cell biology international reports.

[15]  E. Guttes,et al.  Arrest of plasmodial motility during mitosis in Physarum polycephalum. , 1963, Experimental cell research.