Translational Enhancement by an Element Downstream of the Initiation Codon in Escherichia coli*

The translation initiation of Escherichia coli mRNAs is known to be facilitated by a ciselement upstream of the initiation codon, called the Shine-Dalgarno (SD) sequence. This sequence complementary to the 3′ end of 16 S rRNA enhances the formation of the translation initiation complex of the 30 S ribosomal subunit with mRNAs. It has been debated that acis element called the downstream box downstream of the initiation codon, in addition to the SD sequence, facilitates formation of the translation initiation complex; however, conclusive evidence remains elusive. Here, we show evidence that the downstream box plays a major role in the enhancement of translation initiation in concert with SD.

[1]  M. Inouye,et al.  DB or not DB in translation? , 1999, Molecular microbiology.

[2]  M. Inouye,et al.  Downstream box: a hidden translational enhancer , 1998, Molecular microbiology.

[3]  M. Inouye,et al.  Deletion analysis of cspA of Escherichia coli: requirement of the AT‐rich UP element for cspA transcription and the downstream box in the coding region for its cold shock induction , 1997, Molecular microbiology.

[4]  E. Winzeler,et al.  Translation of the leaderless Caulobacter dnaX mRNA , 1997, Journal of bacteriology.

[5]  A G Porter,et al.  Functional importance of RNA interactions in selection of translation initiation codons , 1997, Molecular microbiology.

[6]  U. Bläsi,et al.  Downstream box‐anti‐downstream box interactions are dispensable for translation initiation of leaderless mRNAs. , 1996, The EMBO journal.

[7]  U. RajBhandary,et al.  Ribosome‐initiator tRNA complex as an intermediate in translation initiation in Escherichia coli revealed by use of mutant initiator tRNAs and specialized ribosomes. , 1996, The EMBO journal.

[8]  M. Inouye,et al.  The role of the 5'-end untranslated region of the mRNA for CspA, the major cold-shock protein of Escherichia coli, in cold-shock adaptation , 1996, Journal of bacteriology.

[9]  M. L. Sprengart,et al.  The downstream box: an efficient and independent translation initiation signal in Escherichia coli. , 1996, The EMBO journal.

[10]  M. Inouye,et al.  Cold shock induces a major ribosomal-associated protein that unwinds double-stranded RNA in Escherichia coli. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[11]  M. Dreyfus,et al.  The stability of Escherichia coli lacZ mRNA depends upon the simultaneity of its synthesis and translation. , 1995, The EMBO journal.

[12]  E. Snyder,et al.  High-affinity RNA ligands to Escherichia coli ribosomes and ribosomal protein S1: comparison of natural and unnatural binding sites. , 1995, Biochemistry.

[13]  H. Noller,et al.  Suppression of a cold-sensitive mutation in 16S rRNA by overexpression of a novel ribosome-binding factor, RbfA. , 1995, Genes & development.

[14]  R. Brimacombe,et al.  Prokaryotic translation: the interactive pathway leading to initiation. , 1994, Trends in genetics : TIG.

[15]  M. Sørensen,et al.  Synthesis of proteins in Escherichia coli is limited by the concentration of free ribosomes. Expression from reporter genes does not always reflect functional mRNA levels. , 1993, Journal of molecular biology.

[16]  M. Inouye,et al.  Phenotypic revertant mutations of a new OmpR2 mutant (V203Q) of Escherichia coli lie in the envZ gene, which encodes the OmpR kinase , 1993, Journal of bacteriology.

[17]  Multiple control of Escherichia coli lysyl-tRNA synthetase expression involves a transcriptional repressor and a translational enhancer element. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[18]  M. Gottesman,et al.  Translation of the prophage λ cl transcript , 1992, Cell.

[19]  T. Yura,et al.  Interplay of two cis-acting mRNA regions in translational control of sigma 32 synthesis during the heat shock response of Escherichia coli. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[20]  L. Isaksson,et al.  Codon choice and potential complementarity between mRNA downstream of the initiation codon and bases 1471-1480 in 16S ribosomal RNA affects expression of glnS. , 1991, Nucleic acids research.

[21]  M. L. Sprengart,et al.  The initiation of translation in E. coli: apparent base pairing between the 16srRNA and downstream sequences of the mRNA. , 1990, Nucleic acids research.

[22]  H. Noller,et al.  Probing the assembly of the 3' major domain of 16 S rRNA. Interactions involving ribosomal proteins S2, S3, S10, S13 and S14. , 1988, Journal of molecular biology.

[23]  J. Sylvester,et al.  Differential stringent control of the tandem E. coli ribosomal RNA promoters from the rrnA operon expressed in vivo in multicopy plasmids , 1983, Cell.

[24]  Michael Zuker,et al.  Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information , 1981, Nucleic Acids Res..

[25]  Jeffrey H. Miller.  Experiments in molecular genetics , 1972 .

[26]  R. Kohler,,et al.  Polysomes Extracted from Escherichia coli by Freeze-Thaw-Lysozyme Lysis , 1966, Science.