Growth characteristics and biofilm formation of various spoilage bacteria isolated from fresh produce.

UNLABELLED This study investigated the characteristics of spoilage bacteria isolated from fresh produce including growth at various temperatures, biofilm formation, cell hydrophobicity, and colony spreading. The number of spoilage bacteria present when stored at 35 °C was significantly greater than when stored at lower temperatures, and maximum population size was achieved after 10 h. However, Bacillus pumilus, Dickeya zeae, Pectobacterium carotovorum subsp. Carotovorum Pcc21, and Bacillus pumilus (RDA-R) did not grow at the storage temperature of 5 °C. The biofilm formation by Clavibacter michiganensis, Acinetobacter calcoaceticus, and A. calcoaceticus (RDA-R) are higher than other spoilage bacteria. Biofilm formation showed low correlation between hydrophobicity, and no significant correlation with colony spreading. These results might be used for developing safe storage guidelines for fresh produce at various storage temperatures, and could be basic information on the growth characteristics and biofilm formation properties of spoilage bacteria from fresh produce. PRACTICAL APPLICATION Growth of spoilage bacteria was different depending on the bacteria strains and storage temperature. Between biofilm formation and cell hydrophobicity was low correlation on spoilage bacteria. Therefore, growth characteristics and biofilm formation of spoilage bacteria might be used for developing safe storage guidelines for fresh produce at various storage temperatures.

[1]  Eunjung Roh,et al.  Microbiota on spoiled vegetables and their characterization. , 2013, Journal of food protection.

[2]  A. Lefcourt,et al.  Native microflora in fresh-cut produce processing plants and their potentials for biofilm formation. , 2013, Journal of food protection.

[3]  T. Fang,et al.  Growth kinetics of Listeria monocytogenes and spoilage microorganisms in fresh-cut cantaloupe. , 2013, Food microbiology.

[4]  Sun-Young Lee,et al.  Biofilm formation, attachment, and cell hydrophobicity of foodborne pathogens under varied environmental conditions , 2013, Journal of the Korean Society for Applied Biological Chemistry.

[5]  Ali Ellafi,et al.  Biofilm formation, cell surface hydrophobicity, and fatty acids analysis of starved Salmonella enterica serovar Typhimurium in seawater. , 2012, Foodborne pathogens and disease.

[6]  Koon Hoong Teh,et al.  Biofilm formation by Campylobacter jejuni in controlled mixed-microbial populations. , 2010, International journal of food microbiology.

[7]  C. Michiels,et al.  Biofilm formation and the food industry, a focus on the bacterial outer surface , 2010, Journal of applied microbiology.

[8]  B. Kimura,et al.  Cellular hydrophobicity of Listeria monocytogenes involves initial attachment and biofilm formation on the surface of polyvinyl chloride , 2010, Letters in applied microbiology.

[9]  Rebecca M. Goulter,et al.  Characterisation of Curli Production, Cell Surface Hydrophobicity, Autoaggregation and Attachment Behaviour of Escherichia coli O157 , 2010, Current Microbiology.

[10]  Xianming Shi,et al.  Biofilm formation and food safety in food industries , 2009 .

[11]  A. Fouet,et al.  Biofilm Formation and Cell Surface Properties among Pathogenic and Nonpathogenic Strains of the Bacillus cereus Group , 2009, Applied and Environmental Microbiology.

[12]  M. Valero,et al.  Survival, isolation and characterization of a psychrotrophic Bacillus cereus strain from a mayonnaise-based ready-to-eat vegetable salad. , 2007, Food microbiology.

[13]  C. Kaito,et al.  Colony Spreading in Staphylococcus aureus , 2006, Journal of bacteriology.

[14]  S. Mpuchane,et al.  Microflora of minimally processed frozen vegetables sold in Gaborone, Botswana. , 2006, Journal of food protection.

[15]  U. Romling,et al.  Short communication Biofilm formation and the survival of Salmonella Typhimurium on parsley , 2006 .

[16]  M. Karp,et al.  Adhesion of bacteria to resected human colonic tissue: quantitative analysis of bacterial adhesion and viability. , 2005, Research in microbiology.

[17]  A. Allende,et al.  Microbial and sensory quality of commercial fresh processed red lettuce throughout the production chain and shelf life. , 2004, International journal of food microbiology.

[18]  Joseph F. Frank,et al.  Biofilm Formation and Control in Food Processing Facilities. , 2003, Comprehensive reviews in food science and food safety.

[19]  Lone Gram,et al.  Food spoilage--interactions between food spoilage bacteria. , 2002, International journal of food microbiology.

[20]  R. Donlan,et al.  Biofilms: Microbial Life on Surfaces , 2002, Emerging infectious diseases.

[21]  S. Roller,et al.  Efficacy of chitosan, carvacrol, and a hydrogen peroxide-based biocide against foodborne microorganisms in suspension and adhered to stainless steel. , 2001, Journal of food protection.

[22]  C. Häse,et al.  Flagellum-Independent Surface Migration ofVibrio cholerae and Escherichia coli , 2001, Journal of bacteriology.

[23]  I. Karunasagar,et al.  Biofilm formation by salmonella spp. on food contact surfaces and their sensitivity to sanitizers. , 2001, International journal of food microbiology.

[24]  I. Harper,et al.  Bacterial colonization and biofilm development on minimally processed vegetables , 1998, Journal of applied microbiology.

[25]  Jun Song,et al.  Modified Atmosphere Packaged Cut Iceberg Lettuce: Effect of Temperature and O2 Partial Pressure on Respiration and Quality , 1998 .

[26]  C. Pin,et al.  Cell surface hydrophobicity and attachment of pathogenic and spoilage bacteria to meat surfaces. , 1997, Meat science.

[27]  E. A. Zottola,et al.  Attachment of Listeria monocytogenes to Stainless Steel Surfaces at Various Temperatures and pH Values , 1988 .

[28]  T. A. Roberts,et al.  Predicting microbial growth: growth responses of salmonellae in a laboratory medium as affected by pH, sodium chloride and storage temperature. , 1988, International journal of food microbiology.

[29]  L. Baddour,et al.  Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices , 1985, Journal of clinical microbiology.