3D micro-CT imaging of the postmortem brain

Magnetic resonance microscopy (microMRI) is becoming an important tool for non-destructive analysis of fixed brain tissue. However, unlike MRI, X-ray computed tomography (CT) scans show little native soft tissue contrast. In this paper, we explored the use of contrast enhanced (brains immersion stained in iodinated CT contrast media) micro-CT (microCT) for high resolution 3D imaging of fixed normal and pathological brains, compared to microMRI and standard histopathology. An optimum iodine concentration of 0.27 M resulted in excellent contrast between gray and white matter in normal brain and a wide range of anatomical structures were identified. In glioma bearing mouse brains, there was clear deliniation of tumor margin which closely matched that seen on histopathology sections. microCT tumor volume was strongly correlated with histopathology volume. Our data suggests that microCT image contrast in the immersion-stained brains is related to axonal density and myelin content. Compared to traditional histopathology, our microCT approach is relatively rapid and less labor intensive. In addition, compared to microMRI, microCT is robust and requires much lower equipment and maintenance costs. For simple measurements, such as tumor volume and non-destructive postmortem brain screening, microCT may prove to be a valuable alternative to standard histopathology or microMRI.

[1]  M. Thelen,et al.  Tissue characterization with T1, T2, and proton density values: results in 160 patients with brain tumors. , 1988, Radiology.

[2]  M. Tovi,et al.  Measurements of T1 and T2 over Time in Formalin-Fixed Human Whole-Brain Specimens , 1992 .

[3]  Andreas Breithecker,et al.  Acute rat lung injury: feasibility of assessment with micro-CT. , 2004, Radiology.

[4]  R. Coatney,et al.  Applications of micro-CT and MR microscopy to study pre-clinical models of osteoporosis and osteoarthritis. , 1998, Technology and health care : official journal of the European Society for Engineering and Medicine.

[5]  S. Oesterle,et al.  Percutaneous transmyocardial revascularization. , 1997, Journal of clinical laser medicine & surgery.

[6]  F Eckstein,et al.  [Three-dimensional thickness and volume measurements of the knee joint cartilage using MRI: validation in an anatomical specimen by CT arthrography]. , 1997, RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin.

[7]  Alex J. de Crespigny,et al.  An approach to high resolution diffusion tensor imaging in fixed primate brain , 2007, NeuroImage.

[8]  D. Salat,et al.  Detection of entorhinal layer II using 7Tesla [corrected] magnetic resonance imaging. , 2005, Annals of neurology.

[9]  M. Capecchi,et al.  Virtual Histology of Transgenic Mouse Embryos for High-Throughput Phenotyping , 2006, PLoS genetics.

[10]  G. Fuller,et al.  Utility of postmortem magnetic resonance imaging in clinical neuropathology. , 1994, Archives of pathology & laboratory medicine.

[11]  L W Hedlund,et al.  Histology by magnetic resonance microscopy. , 1993, Magnetic resonance quarterly.

[12]  H. Redmond,et al.  Vascular endothelial growth factor stimulates bone repair by promoting angiogenesis and bone turnover , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[13]  Pat Levitt,et al.  Three-Dimensional High-Resolution Diffusion Tensor Imaging and Tractography of the Developing Rabbit Brain , 2007, Developmental Neuroscience.

[14]  E L Ritman,et al.  Enhanced coronary vasa vasorum neovascularization in experimental hypercholesterolemia. , 1998, The Journal of clinical investigation.

[15]  G A Johnson,et al.  In vitro MR microscopy of the hippocampus in Alzheimer's disease , 1993, Neurology.

[16]  E L Ritman,et al.  Three-dimensional microcomputed tomography of renal vasculature in rats. , 1998, Hypertension.

[17]  Edith V. Sullivan,et al.  Postmortem MR imaging of formalin-fixed human brain , 2004, NeuroImage.

[18]  Alex J. de Crespigny,et al.  The effects of brain tissue decomposition on diffusion tensor imaging and tractography , 2007, NeuroImage.

[19]  J. Frahm Rapid FLASH NMR imaging , 1987, Naturwissenschaften.

[20]  M. F. Reiser,et al.  Dreidimensionale Dicken- und Volumenbestimmung des Kniegelenkknorpels in der MRT: Validierung am anatomischen Präparat mittels CT-Arthrographie , 1997 .

[21]  L. Hedlund,et al.  Morphologic phenotyping with MR microscopy: the visible mouse. , 2002, Radiology.

[22]  J. W. Shek,et al.  Atlas of the Rabbit Brain and Spinal Cord , 1985 .

[23]  S. Jewell,et al.  Copyright © American Society for Investigative Pathology Review Effect of Fixatives and Tissue Processing on the Content and Integrity of Nucleic Acids , 2022 .

[24]  G. Johnson,et al.  3-Dimensional visualization of lesions in rat brain using magnetic resonance imaging microscopy. , 1999, Neuroreport.

[25]  L W Hedlund,et al.  Magnetic Resonance Microscopy-A New Tool for the Toxicologic Pathologist , 1996, Toxicologic pathology.

[26]  P. Rüegsegger,et al.  Morphometric analysis of human bone biopsies: a quantitative structural comparison of histological sections and micro-computed tomography. , 1998, Bone.

[27]  N. van Bruggen,et al.  Quantification of cortical bone loss and repair for therapeutic evaluation in collagen-induced arthritis, by micro-computed tomography and automated image analysis. , 2004, Arthritis and rheumatism.