Cattle Can Be a Reservoir of Sorbitol-Fermenting Shiga Toxin-Producing Escherichia coli O157:H−Strains and a Source of Human Diseases

ABSTRACT Using the immunomagnetic separation procedure, we isolated sorbitol-fermenting (SF) Shiga toxin-producing Escherichia coli (STEC) O157:H− strains from two patients, one with hemolytic-uremic syndrome and the other with diarrhea, and from a dairy cow epidemiologically associated with the patients. The phenotypic and genotypic characteristics of all isolates were identical or closely related. Moreover, the bovine isolate showed a clonal relatedness to SF STEC O157:H− strains isolated from patients in Germany and the Czech Republic from 1988 to 1998. This is the first evidence that cattle can be a reservoir of SF STEC O157:H− and a source of human diseases.

[1]  A. Caprioli,et al.  Typing of Intimin Genes in Human and Animal Enterohemorrhagic and Enteropathogenic Escherichia coli: Characterization of a New Intimin Variant , 2000, Infection and Immunity.

[2]  H. Karch,et al.  Molecular characteristics and epidemiological significance of Shiga toxin-producing Escherichia coli O26 strains. , 2000, Journal of clinical microbiology.

[3]  H. Karch,et al.  Epidemiology and diagnosis of Shiga toxin-producing Escherichia coli infections. , 1999, Diagnostic microbiology and infectious disease.

[4]  H. Karch,et al.  A large outbreak of hemolytic uremic syndrome caused by an unusual sorbitol-fermenting strain of Escherichia coli O157:H-. , 1999, The Journal of infectious diseases.

[5]  S. Martin,et al.  Temporal and geographical distributions of reported cases of Escherichia coli O157[ratio ]H7 infection in Ontario , 1999, Epidemiology and Infection.

[6]  H. Karch,et al.  Isolation and Characterization of Sorbitol-Fermenting Shiga Toxin (Verocytotoxin)-Producing Escherichia coli O157:Hßtrains in the Czech Republic , 1998, Journal of Clinical Microbiology.

[7]  B. Swaminathan,et al.  Molecular characterization of the gene encoding H antigen in Escherichia coli and development of a PCR-restriction fragment length polymorphism test for identification of E. coli O157:H7 and O157:NM , 1997, Journal of clinical microbiology.

[8]  H. Karch,et al.  EspP, a novel extracellular serine protease of enterohaemorrhagic Escherichia coli O157:H7 cleaves human coagulation factor V , 1997, Molecular microbiology.

[9]  H. Karch,et al.  A gene cluster closely related to type II secretion pathway operons of gram-negative bacteria is located on the large plasmid of enterohemorrhagic Escherichia coli O157 strains. , 1997, FEMS microbiology letters.

[10]  H. Karch,et al.  KatP, a novel catalase-peroxidase encoded by the large plasmid of enterohaemorrhagic Escherichia coli O157:H7. , 1996, Microbiology.

[11]  H. Karch,et al.  Isolation of enterohemorrhagic Escherichia coli O157 strains from patients with hemolytic-uremic syndrome by using immunomagnetic separation, DNA-based methods, and direct culture , 1996, Journal of clinical microbiology.

[12]  F. Gunzer,et al.  Analysis of the enterohemorrhagic Escherichia coli O157 DNA region containing lambdoid phage gene p and Shiga-like toxin structural genes , 1996, Applied and environmental microbiology.

[13]  L. Beutin,et al.  Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933 , 1995, Infection and immunity.

[14]  H. Karch,et al.  Prevalence of attaching and effacing Escherichia coli in stool samples from patients and controls. , 1994, Zentralblatt fur Bakteriologie : international journal of medical microbiology.

[15]  A. Borczyk,et al.  Evidence of direct transmission of Escherichia coli O157:H7 infection between calves and a human. , 1993, The Journal of infectious diseases.

[16]  J. Heesemann,et al.  Clonal structure and pathogenicity of Shiga-like toxin-producing, sorbitol-fermenting Escherichia coli O157:H- , 1993, Journal of clinical microbiology.

[17]  F. Gunzer,et al.  Molecular detection of sorbitol-fermenting Escherichia coli O157 in patients with hemolytic-uremic syndrome , 1992, Journal of clinical microbiology.

[18]  H. Lior,et al.  Extended phage-typing scheme for Escherichia coli 0157:H7 , 1990, Epidemiology and Infection.

[19]  S. Formal,et al.  ESCHERICHIA COLI 0157:H7 STRAINS ASSOCIATED WITH HAEMORRHAGIC COLITIS IN THE UNITED STATES PRODUCE A SHIGELLA DYSENTERIAE 1 (SHIGA) LIKE CYTOTOXIN , 1983, The Lancet.