Cervical sympathectomy causes alveolar bone loss in an experimental rat model.

BACKGROUND AND OBJECTIVE Periodontal disease, a pathological destructive inflammatory condition, is characterized by alveolar bone loss. Recent studies have suggested a correlation between the sympathetic nervous system and bone remodeling. To confirm the importance of the sympathetic nervous system in bone resorption, we investigated the effects of superior cervical ganglionectomy and oral challenge with Porphyromonas gingivalis on alveolar bone loss in rats. MATERIAL AND METHODS Rats were divided into three groups: group A underwent a sham operation as the control group; group B underwent superior cervical ganglionectomy; and group C underwent a sham operation and oral challenge with P. gingivalis. Horizontal alveolar bone loss was evaluated by measuring the distance between the cemento-enamel junction and the alveolar bone crest. Cytokine gene expression in the gingival tissues was assessed using reverse transcription-polymerase chain reaction analyses. The furcation areas of the mandibular molars were examined histologically. RESULTS Both superior cervical ganglionectomy and oral challenge with P. gingivalis resulted in accelerated alveolar bone loss. Gingival tissues in the superior cervical ganglionectomy group showed increased expression of the cytokines interleukin-1 alpha, tumor necrosis factor-alpha and interleukin-6. The density of neuropeptide Y-immunoreactive fibers was decreased following superior cervical ganglionectomy. Osteoclasts were observed in the superior cervical ganglionectomy and P. gingivalis-challenged groups. CONCLUSION Both superior cervical ganglionectomy and oral challenge with P. gingivalis induced alveolar bone loss. These results provide new information on the occurrence of alveolar bone loss, in that both oral challenge with P. gingivalis and superior cervical ganglionectomy are important accelerating factors for alveolar bone loss. Thus, we suggest that the sympathetic nervous system is linked with the prevention of alveolar bone loss.

[1]  Tetsuo Kobayashi,et al.  The r40-kDa outer membrane protein human monoclonal antibody protects against Porphyromonas gingivalis-induced bone loss in rats. , 2007, Journal of periodontology.

[2]  K. Tsukinoki,et al.  Restraint stress enhances alveolar bone loss in an experimental rat model. , 2006, Journal of periodontal research.

[3]  S. R. Haug,et al.  Modulation of Dental Inflammation by the Sympathetic Nervous System , 2006, Journal of dental research.

[4]  C. Perrone-Capano,et al.  Gene expression pathways induced by axotomy and decentralization of rat superior cervical ganglion neurons , 2006, The European journal of neuroscience.

[5]  P. Baker.  Genetic Control of the Immune Response in Pathogenesis. , 2005, Journal of periodontology.

[6]  J. Steinle,et al.  Cervical sympathectomy causes photoreceptor-specific cell death in the rat retina , 2005, Autonomic Neuroscience.

[7]  F. Fonnum,et al.  Chronic glycine treatment inhibits ligature-induced periodontal disease in Wistar rats. , 2005, Journal of periodontal research.

[8]  N. Buduneli,et al.  Effects of combined systemic administration of low-dose doxycycline and alendronate on endotoxin-induced periodontitis in rats. , 2004, Journal of periodontology.

[9]  S. R. Haug,et al.  IL-1α and TNF-α Expression in Rat Periapical Lesions and Dental Pulp after Unilateral Sympathectomy , 2004, Neuroimmunomodulation.

[10]  Xiao-Min Wang,et al.  Electro-acupuncture stimulation protects dopaminergic neurons from inflammation-mediated damage in medial forebrain bundle-transected rats , 2004, Experimental Neurology.

[11]  N. Yoshinari,et al.  Effect of restraint stress on the progression of experimental periodontitis in rats. , 2004, Journal of periodontology.

[12]  R. Elde,et al.  Distribution of CGRP-, VIP-, DβH-, SP-, and NPY-immunoreactive nerves in the periosteum of the rat , 1991, Cell and Tissue Research.

[13]  C. Marcelli,et al.  Sympathetic nervous system as transmitter of mechanical loading in bone. , 2003, Joint, bone, spine : revue du rhumatisme.

[14]  A. Fukushima,et al.  Differential Expression and Signaling of IFN‐γ in the Conjunctiva between Lewis and Brown Norway Rats , 2003 .

[15]  D. Cardinali,et al.  Effect of unilateral superior cervical ganglionectomy on mandibular bone in rats. , 2003, Neuro endocrinology letters.

[16]  S. R. Haug,et al.  Effects of sympathectomy on experimentally induced pulpal inflammation and periapical lesions in rats , 2003, Neuroscience.

[17]  I. Fristad,et al.  Sympathectomy causes increased root resorption after orthodontic tooth movement in rats: immunohistochemical study , 2003, Cell and Tissue Research.

[18]  A. Fukushima,et al.  Differential expression and signaling of IFN-gamma in the conjunctiva between Lewis and Brown Norway rats. , 2003, Microbiology and immunology.

[19]  B. Chandrasekar,et al.  In vivo induction of proinflammatory cytokines in mouse tissue by Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans. , 2002, Oral microbiology and immunology.

[20]  J. O'Callaghan,et al.  Neuroendocrine aspects of the response to stress. , 2002, Metabolism: clinical and experimental.

[21]  U. Skalerič,et al.  Influence of restraint stress on ligature-induced periodontitis in rats. , 2002, European Journal of Oral Sciences.

[22]  G. Chrousos,et al.  The sympathetic nerve--an integrative interface between two supersystems: the brain and the immune system. , 2000, Pharmacological reviews.

[23]  P. Opstad,et al.  Glucocorticoid receptor antagonist RU 486 treatment reduces periodontitis in Fischer 344 rats. , 2000, Journal of periodontal research.

[24]  D. Cardinali,et al.  Effect of unilateral superior cervical ganglionectomy on bone mineral content and density of rat's mandible. , 2000, Journal of the autonomic nervous system.

[25]  B. Baroukh,et al.  Chemical sympathectomy impairs bone resorption in rats: a role for the sympathetic system on bone metabolism. , 1999, Bone.

[26]  G. Chrousos,et al.  Stress Hormones, Th1/Th2 patterns, Pro/Anti-inflammatory Cytokines and Susceptibility to Disease , 1999, Trends in Endocrinology & Metabolism.

[27]  R. Genco,et al.  Relationship of stress, distress and inadequate coping behaviors to periodontal disease. , 1999, Journal of periodontology.

[28]  H. Yamato,et al.  Combined effect of cigarette smoke and mineral fibers on the gene expression of cytokine mRNA. , 1999, Environmental health perspectives.

[29]  N. Kawashima,et al.  Expression of bone-resorptive and regulatory cytokines in murine periapical inflammation. , 1999, Archives of oral biology.

[30]  R. Genco,et al.  Models to evaluate the role of stress in periodontal disease. , 1998, Annals of periodontology.

[31]  M. Sironi,et al.  The sympathetic nervous system tonically inhibits peripheral interleukin-1β and interleukin-6 induction by central lipopolysaccharide , 1998, Neuroscience.

[32]  K. Maeda,et al.  Immunohistochemical examination of the distribution of macrophages and CGRP-immunoreactive nerve fibers in induced rat periapical lesions. , 1997, Endodontics & dental traumatology.

[33]  R. Murison,et al.  Emotional stress effects on immunity, gingivitis and periodontitis. , 1996, European journal of oral sciences.

[34]  M. Swift,et al.  Effect of ageing on responses of nerve fibres to pulpal inflammation in rat molars analysed by quantitative immunocytochemistry. , 1992, Archives of oral biology.

[35]  I. J. Singh,et al.  The early effects of surgical sympathectomy on bone resorption in the rat incisor socket. , 1990, Archives of oral biology.

[36]  I. J. Singh,et al.  Effect of surgical sympathectomy on bone remodeling at rat incisor and molar root sockets , 1987, The Anatomical record.

[37]  D. Cardinali,et al.  Role of the sympathetic nervous system in the control of thyroid compensatory growth of normal and hypophysectomized rats. , 1985, Neuroendocrinology.

[38]  F. Sundler,et al.  Neuropeptide Y: occurrence and distribution in dental pulps. , 1984, Acta odontologica Scandinavica.

[39]  D. Chiego,et al.  Modulation of osteoblastic activity by sensory and autonomic innervation of bone. , 1982, Progress in clinical and biological research.