Liver Tissue Decellularization as a Promising Porous Scaffold Processing Technology for Tissue Engineering and Regenerative Medicine

doi: 10.17691/stm2015.7.4.01 Received march 20, 2015М.М. Bobrova, Clinical Research Assistant;L.А. Safonova, Clinical Research Assistant;О.I. Agapova, Researcher, Bionanotechnology Laboratory;M.E. Krasheninnikov, Phd, Senior Researcher, Cellular Technology Laboratory;M.Yu. Shagidulin, md, Phd, Head of the department of Experimental Transplantology and Artificial organs;I.I. Agapov, dSc, Professor, Head of Bionanotechnology LaboratoryAcademician V.i. Shumakov Federal Research Center of Transplantology and Artificial organs, ministry of Health of the Russian Federation, 1 Stchukinskaya St., moscow, 123182, Russian FederationThe aim of the investigation was to study mechanical and biological properties of decellularized liver tissue when used as a porous matrix in regenerative medicine.Materials and Methods. Three groups of liver samples were prepared by decellularization using a perfusion solution with different concentrations of Triton X-100. The vascular network was visualized by perfusion of 0.5% blue dextran solution. We used histological tissue staining, optical microscopy and scanning electron microscopy. Human hepatocarcinoma cell line Hep-G

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