Immobilization–stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus

[1]  Ping Wang,et al.  Cofactor regeneration for sustainable enzymatic biosynthesis. , 2007, Biotechnology advances.

[2]  Roberto Fernandez-Lafuente,et al.  Improvement of enzyme activity, stability and selectivity via immobilization techniques , 2007 .

[3]  R. Fernández-Lafuente,et al.  Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports: Correlation between enzyme–support linkages and thermal stability , 2007 .

[4]  W. Cleland,et al.  Statistical analysis of enzyme kinetic data. , 2006, Methods in enzymology.

[5]  W. Cleland Determining the chemical mechanisms of enzyme-catalyzed reactions by kinetic studies. , 2006, Advances in enzymology and related areas of molecular biology.

[6]  J. Kaur,et al.  Immobilization and stability studies of a lipase from thermophilic Bacillus sp: The effect of process parameters on immobilization of enzyme , 2006 .

[7]  G. Zanin,et al.  Characterization of Thermoanaerobacter cyclomaltodextrin glucanotransferase immobilized on glyoxyl-agarose , 2006 .

[8]  R. Fernández-Lafuente,et al.  Improvement of the stability of alcohol dehydrogenase by covalent immobilization on glyoxyl-agarose. , 2006, Journal of biotechnology.

[9]  Ruey-an Doong,et al.  Glutamate optical biosensor based on the immobilization of glutamate dehydrogenase in titanium dioxide sol-gel matrix. , 2006, Biosensors & bioelectronics.

[10]  R. Fernández-Lafuente,et al.  Glyoxyl agarose: A fully inert and hydrophilic support for immobilization and high stabilization of proteins , 2006 .

[11]  Runu Chakraborty,et al.  A biosensor based on co-immobilized L-glutamate oxidase and L-glutamate dehydrogenase for analysis of monosodium glutamate in food. , 2006, Biosensors & bioelectronics.

[12]  S. Díaz,et al.  Gene cloning, heterologous overexpression and optimized refolding of the NAD-glutamate dehydrogenase from Haloferax mediterranei , 2006, Extremophiles.

[13]  R. Fernández-Lafuente,et al.  Stabilization of a formate dehydrogenase by covalent immobilization on highly activated glyoxyl-agarose supports. , 2006, Biomacromolecules.

[14]  O. Abián,et al.  Some special features of glyoxyl supports to immobilize proteins , 2005 .

[15]  R. Fernández-Lafuente,et al.  Advantages of the pre-immobilization of enzymes on porous supports for their entrapment in sol-gels. , 2005, Biomacromolecules.

[16]  I. Tothill,et al.  Urease–glutamic dehydrogenase biosensor for screening heavy metals in water and soil samples , 2004, Analytical and bioanalytical chemistry.

[17]  I. Tothill,et al.  Development of urease and glutamic dehydrogenase amperometric assay for heavy metals screening in polluted samples. , 2004, Biosensors & bioelectronics.

[18]  U. Kragl,et al.  Recent Developments in NAD(P)H Regeneration for Enzymatic Reductions in One- and Two-Phase Systems , 2004 .

[19]  M. Sung,et al.  Thermostable glutamate dehydrogenase from a commensal thermophile, Symbiobacterium toebii; overproduction, characterization, and application , 2003 .

[20]  Huimin Zhao,et al.  Regeneration of cofactors for use in biocatalysis. , 2003, Current opinion in biotechnology.

[21]  Huimin Zhao,et al.  Recent developments in pyridine nucleotide regeneration. , 2003, Current opinion in biotechnology.

[22]  C. Pire,et al.  Denaturation Studies by Fluorescence and Quenching of Thermophilic Protein NAD+-Glutamate Dehydrogenase from Thermus thermophilus HB8 , 2003, Journal of protein chemistry.

[23]  M D Leonida,et al.  Redox enzymes used in chiral syntheses coupled to coenzyme regeneration. , 2001, Current medicinal chemistry.

[24]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[25]  D. Cowan,et al.  Biomolecular stability and life at high temperatures , 2000, Cellular and Molecular Life Sciences CMLS.

[26]  W. Hummel Large-scale applications of NAD(P)-dependent oxidoreductases: recent developments. , 1999, Trends in biotechnology.

[27]  J. Lebbink,et al.  Glutamate dehydrogenase from hyperthermophilic Bacteria and Archaea: determinants of thermostability and catalysis at extremely high temperatures , 1999 .

[28]  J. Lebbink,et al.  Insights into the molecular basis of thermal stability from the analysis of ion-pair networks in the glutamate dehydrogenase family. , 1998, European journal of biochemistry.

[29]  J. Lebbink,et al.  Engineering activity and stability of Thermotoga maritima glutamate dehydrogenase. I. Introduction of a six-residue ion-pair network in the hinge region. , 1998, Journal of molecular biology.

[30]  T. Ohshima,et al.  Enzymological Characteristics of the Hyperthermostable NAD-Dependent Glutamate Dehydrogenase from the Archaeon Pyrobaculum islandicum and Effects of Denaturants and Organic Solvents , 1998, Applied and Environmental Microbiology.

[31]  R. Huber,et al.  A dissimilatory sirohaem-sulfite-reductase-type protein from the hyperthermophilic archaeon Pyrobaculum islandicum. , 1998, Microbiology.

[32]  S. Fujiwara,et al.  Sequence analysis of glutamate dehydrogenase (GDH) from the hyperthermophilic archaeon Pyrococcus sp. KOD1 and comparison of the enzymatic characteristics of native and recombinant GDHs , 1998, Molecular and General Genetics MGG.

[33]  J. Ferrer,et al.  NAD‐specific glutamate dehydrogenase from Thermus thermophilus HB8: purification and enzymatic properties , 1998 .

[34]  I. Steen,et al.  Purification and properties of an extremely thermostable NADP+-specific glutamate dehydrogenase from Archaeoglobus fulgidus , 1997, Archives of Microbiology.

[35]  D. Cowan,et al.  Thermophilic proteins: stability and function in aqueous and organic solvents. , 1997, Comparative biochemistry and physiology. Part A, Physiology.

[36]  S. Knapp,et al.  Crystal structure of glutamate dehydrogenase from the hyperthermophilic eubacterium Thermotoga maritima at 3.0 A resolution. , 1997, Journal of molecular biology.

[37]  W. D. de Vos,et al.  Glutamate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima: molecular characterization and phylogenetic implications , 1997, Extremophiles.

[38]  S. Higuchi,et al.  Properties of glutamate dehydrogenase and its involvement in alanine production in a hyperthermophilic archaeon, Thermococcus profundus. , 1995, Journal of biochemistry.

[39]  K. S. Yip,et al.  Insights into thermal stability from a comparison of the glutamate dehydrogenases from Pyrococcus furiosus and Thermococcus litoralis. , 1995, European journal of biochemistry.

[40]  B. McBride,et al.  Nucleotide sequence of a Porphyromonas gingivalis gene encoding a surface-associated glutamate dehydrogenase and construction of a glutamate dehydrogenase-deficient isogenic mutant , 1994, Infection and immunity.

[41]  F. Bossa,et al.  The amino acid sequence of glutamate dehydrogenase fromPyrococcus furiosus, a hyperthermophilic archaebacterium , 1994, Journal of protein chemistry.

[42]  R. Daniel,et al.  Glutamate dehydrogenase from the extremely thermophilic archaebacterial isolate AN1. , 1993, Biochimica et biophysica acta.

[43]  M. de Pedro,et al.  Development of Thermus-Escherichia shuttle vectors and their use for expression of the Clostridium thermocellum celA gene in Thermus thermophilus , 1992, Journal of bacteriology.

[44]  D. Cowan,et al.  Correlation between microbial protein thermostability and resistance to denaturation in aqueous-organic solvent 2-phase systems , 1989 .

[45]  R. M. Blanco,et al.  Stabilization of enzymes by multipoint covalent attachment to agarose-aldehyde gels. Borohydride reduction of trypsin-agarose derivatives , 1989 .

[46]  J. Guisán Aldehyde-agarose gels as activated supports for immobilization-stabilization of enzymes , 1988 .

[47]  F. Neidhardt,et al.  Escherichia Coli and Salmonella: Typhimurium Cellular and Molecular Biology , 1987 .

[48]  M. M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.

[49]  D. Haltrich,et al.  Lactose hydrolysis and formation of galactooligosaccharides by a novel immobilized β-galactosidase from the thermophilic fungus Talaromyces thermophilus , 2006 .

[50]  D. Haltrich,et al.  Lactose hydrolysis and formation of galactooligosaccharides by a novel immobilized beta-galactosidase from the thermophilic fungus Talaromyces thermophilus. , 2006, Applied Biochemistry and Biotechnology.

[51]  C. Wandrey Biochemical reaction engineering for redox reactions. , 2004, Chemical record.

[52]  J. García,et al.  One-step purification, covalent immobilization, and additional stabilization of a thermophilic poly-His-tagged beta-galactosidase from Thermus sp. strain T2 by using novel heterofunctional chelate-epoxy Sepabeads. , 2003, Biomacromolecules.

[53]  R. Fernández-Lafuente,et al.  Biotransformations catalyzed by multimeric enzymes: stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions. , 2001, Biomacromolecules.

[54]  J. DiRuggiero,et al.  Glutamate dehydrogenases from hyperthermophiles. , 2001, Methods in Enzymology.

[55]  J. DiRuggiero,et al.  [3] Glutamate dehydrogenases from hyperthermophiles , 2001 .

[56]  W. Hummel New alcohol dehydrogenases for the synthesis of chiral compounds. , 1997, Advances in biochemical engineering/biotechnology.

[57]  S. Higuchi,et al.  Molecular cloning, nucleotide sequence and expression in Escherichia coli of hyperthermophilic glutamate dehydrogenase gene from Thermococcus profundus , 1997 .

[58]  D Compagnone,et al.  Amperometric ammonium ion and urea determination with enzyme-based probes. , 1996, Biosensors & bioelectronics.

[59]  R. Jaenicke,et al.  Structure and stability of hyperstable proteins: glycolytic enzymes from hyperthermophilic bacterium Thermotoga maritima. , 1996, Advances in protein chemistry.

[60]  F. Bossa,et al.  The protein sequence of glutamate dehydrogenase from Sulfolobus solfataricus, a thermoacidophilic archaebacterium. Is the presence of N-epsilon-methyllysine related to thermostability? , 1992, European journal of biochemistry.

[61]  M. N. Gupta Thermostabilization of proteins , 1991 .

[62]  M. Ashburner A Laboratory manual , 1989 .

[63]  G. Whitesides,et al.  Cofactor regeneration for enzyme-catalysed synthesis. , 1988, Biotechnology & genetic engineering reviews.

[64]  A. Klibanov Approaches to enzyme stabilization. , 1983, Biochemical Society transactions.

[65]  A. Klibanov,et al.  Enzyme stabilization by immobilization. , 1979, Analytical biochemistry.

[66]  B. Austen,et al.  5 Glutamate Dehydrogenases , 1975 .

[67]  B. Goldin,et al.  L-Glutamate Dehydrogenases* , 1971 .

[68]  U. K. Laemmli,et al.  Cleavage of structural proteins during , 1970 .