The Design of a High Efficient DNA In-Situ Synthesis Apparatus Based on the Principle of Typography Technique

The paper describes a novel machine for in-situ synthesis of DNA Micro-arrays based on the use of typography technique. This apparatus was designed to incorporate platform for imprinting, precision printing stencil, one-way positioning device, manipulator, and liquid & gas delivery part and control part. The programmable logic controller was employed to control operation steps involving in biochip synthesis including locating and imprinting, and delivering the specific liquid. Finally, the control precision of system is measured using three coordinated instrument: the accuracy of location in X, Y axis is 5 µm, accuracy of printing template is 7 µm, and the repeated printing accuracy of whole machine is 16 µm. The result indicates that the design can completely meet the requirement of in situ synthesis of biochip based on the principle of typography printing.