Matched Backprojection Operator for Combined Scanning Transmission Electron Microscopy Tilt- and Focal Series

Abstract Combined tilt- and focal series scanning transmission electron microscopy is a recently developed method to obtain nanoscale three-dimensional (3D) information of thin specimens. In this study, we formulate the forward projection in this acquisition scheme as a linear operator and prove that it is a generalization of the Ray transform for parallel illumination. We analytically derive the corresponding backprojection operator as the adjoint of the forward projection. We further demonstrate that the matched backprojection operator drastically improves the convergence rate of iterative 3D reconstruction compared to the case where a backprojection based on heuristic weighting is used. In addition, we show that the 3D reconstruction is of better quality.

[1]  W. Baumeister,et al.  Perspectives of molecular and cellular electron tomography. , 1997, Journal of structural biology.

[2]  Niels de Jonge,et al.  Three-dimensional locations of gold-labeled proteins in a whole mount eukaryotic cell obtained with 3nm precision using aberration-corrected scanning transmission electron microscopy. , 2011, Journal of structural biology.

[3]  R. Cooke Real and Complex Analysis , 2011 .

[4]  P. Midgley,et al.  Electron tomography and holography in materials science. , 2009, Nature materials.

[5]  Jose-Jesus Fernandez,et al.  Computational methods for electron tomography. , 2012, Micron.

[6]  G. Tendeloo,et al.  Three-dimensional atomic imaging of crystalline nanoparticles , 2011, Nature.

[7]  G A Zampighi,et al.  Conical tomography of freeze-fracture replicas: a method for the study of integral membrane proteins inserted in phospholipid bilayers. , 2005, Journal of structural biology.

[8]  J. Frank,et al.  Double-tilt electron tomography. , 1995, Ultramicroscopy.

[9]  Kees Joost Batenburg,et al.  Dart: A Fast Heuristic Algebraic Reconstruction Algorithm for Discrete Tomography , 2007, 2007 IEEE International Conference on Image Processing.

[10]  Angus I. Kirkland,et al.  Three-dimensional imaging by optical sectioning in the aberration-corrected scanning transmission electron microscope , 2009, Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences.

[11]  Richard D. Leapman,et al.  Nanoscale 3D cellular imaging by axial scanning transmission electron tomography , 2009, Nature Methods.

[12]  Tim Dahmen,et al.  Combined Scanning Transmission Electron Microscopy Tilt- and Focal Series , 2014, Microscopy and Microanalysis.

[13]  Niels de Jonge,et al.  The Three-Dimensional Point Spread Function of Aberration-Corrected Scanning Transmission Electron Microscopy , 2011, Microscopy and Microanalysis.

[14]  J M Carazo,et al.  3D reconstruction in electron microscopy using ART with smooth spherically symmetric volume elements (blobs). , 1998, Ultramicroscopy.

[15]  R M Lewitt,et al.  Multidimensional digital image representations using generalized Kaiser-Bessel window functions. , 1990, Journal of the Optical Society of America. A, Optics and image science.

[16]  Niels de Jonge,et al.  Optimized Deconvolution for Maximum Axial Resolution in Three-Dimensional Aberration-Corrected Scanning Transmission Electron Microscopy , 2011, Microscopy and Microanalysis.

[17]  Niels de Jonge,et al.  Whole-Cell Analysis of Low-Density Lipoprotein Uptake by Macrophages Using STEM Tomography , 2013, PloS one.

[18]  R. Bracewell Strip Integration in Radio Astronomy , 1956 .

[19]  Gengsheng Lawrence Zeng,et al.  Unmatched projector/backprojector pairs in an iterative reconstruction algorithm , 2000, IEEE Transactions on Medical Imaging.

[20]  David A Muller,et al.  Analytic derivation of optimal imaging conditions for incoherent imaging in aberration-corrected electron microscopes. , 2008, Ultramicroscopy.

[21]  Kazuhiro Aoyama,et al.  STEM tomography for thick biological specimens. , 2008, Ultramicroscopy.

[22]  Zachary H. Levine,et al.  Submicron imaging of buried integrated circuit structures using scanning confocal electron microscopy , 2002 .

[23]  Niels de Jonge,et al.  Three-Dimensional Scanning Transmission Electron Microscopy of Biological Specimens , 2010, Microscopy and Microanalysis.

[24]  Per Christian Hansen,et al.  Semi-convergence properties of Kaczmarz’s method , 2014 .

[25]  F. Natterer The Mathematics of Computerized Tomography , 1986 .

[26]  C. Kübel,et al.  Recent Advances in Electron Tomography: TEM and HAADF-STEM Tomography for Materials Science and Semiconductor Applications , 2005, Microscopy and Microanalysis.

[27]  Kees Joost Batenburg,et al.  Electron tomography based on a total variation minimization reconstruction technique , 2012 .

[28]  Veit Elser,et al.  Breaking the Crowther limit: combining depth-sectioning and tilt tomography for high-resolution, wide-field 3D reconstructions. , 2014, Ultramicroscopy.

[29]  S. Pennycook,et al.  Depth sectioning with the aberration-corrected scanning transmission electron microscope. , 2006, Proceedings of the National Academy of Sciences of the United States of America.