Expression of BMP-4 mRNA during distraction osteogenesis in rabbits.

We characterized the presence and localization of bone morphogenetic protein 4 (BMP-4) mRNA in the regenerating tissues produced by distraction osteogenesis following tibial osteotomy in the rabbit. The findings are consistent with previous reports on the localization of BMP-4 mRNA expression during fracture repair. The BMP-4 gene is expressed by less differentiated osteoprogenitor cells (fibroblastic mesenchymal cells and preosteoblasts), and not by fully differentiated osteoblasts. BMP-4 gene expression is localized in callus-forming tissue (muscle, periosteum) during callus formation. Our observations suggest that the BMP-4 gene product is one of the local contributing factors in regulating bone and cartilage formation in distraction osteogenesis.

[1]  K. Takagi,et al.  A bovine low molecular weight bone morphogenetic protein (BMP) fraction. , 1982, Clinical orthopaedics and related research.

[2]  K. Takaoka,et al.  Transient and localized expression of bone morphogenetic protein 4 messenger RNA during fracture healing , 1994, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[3]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.

[4]  M. Urist.  Bone: Formation by Autoinduction , 1965, Science.

[5]  S. Nomura,et al.  Localization of the mRNA for bone matrix proteins during fracture healing as determined by in situ hybridization , 1994, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[6]  F. Hughes,et al.  The effects of bone morphogenetic protein-2, -4, and -6 on differentiation of rat osteoblast cells in vitro. , 1995, Endocrinology.

[7]  T. Goto,et al.  Bone lengthening in rabbits by callus distraction. The role of periosteum and endosteum. , 1988, The Journal of bone and joint surgery. British volume.

[8]  G A Ilizarov,et al.  The tension-stress effect on the genesis and growth of tissues. Part I. The influence of stability of fixation and soft-tissue preservation. , 1989, Clinical orthopaedics and related research.

[9]  J. Wozney,et al.  The bone morphogenetic protein family and osteogenesis , 1992, Molecular reproduction and development.

[10]  B. Mckibbin,et al.  The biology of fracture healing in long bones. , 1978, The Journal of bone and joint surgery. British volume.

[11]  E. Vuorio,et al.  Gene expression during bone repair. , 1993, Clinical orthopaedics and related research.

[12]  Y. Jin,et al.  An immunocytochemical study of bone morphogenetic protein in experimental fracture healing of the rabbit mandible. , 1994, Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih.

[13]  G A Ilizarov,et al.  Clinical application of the tension-stress effect for limb lengthening. , 1990, Clinical orthopaedics and related research.

[14]  G S Beaupré,et al.  Mechanobiologic influences in long bone cross-sectional growth. , 1993, Bone.

[15]  V. Rosen,et al.  Purification and molecular cloning of a novel group of BMPs and localization of BMP mRNA in developing bone. , 1989, Connective tissue research.

[16]  H. Anderson,et al.  The mechanism of bone induction and bone healing by human osteosarcoma cell extracts. , 1994, Clinical orthopaedics and related research.

[17]  J. Aronson,et al.  The histology of distraction osteogenesis using different external fixators. , 1989, Clinical orthopaedics and related research.

[18]  D. Carter,et al.  Long bone geometry and strength in adult BMP-5 deficient mice. , 1995, Bone.

[19]  A. Weiland,et al.  Immunolocalization and expression of bone morphogenetic proteins 2 and 4 in fracture healing , 1995, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[20]  V. Rosen,et al.  The BMP proteins in bone formation and repair. , 1992, Trends in genetics : TIG.

[21]  B. Hogan,et al.  Bone morphogenetic proteins: multifunctional regulators of vertebrate development. , 1996, Genes & development.

[22]  J. Triffitt,et al.  The effect of decalcified bone matrix on the osteogenic potential of bone marrow. , 1986, Clinical orthopaedics and related research.