Human Tumors and the Epidermal Growth Factor Receptor by β and α Factors Synthesis of Messenger RNAs for Transforming Growth

Human tumors and tumor cell lines were analyzed for the presence of mRNA coding for transforming growth factors a and ß(TGF-a and -ft) and the epidermal growth factor receptor. TGF-a mRNA was not detect able in hematopoietic tumor cell lines but was found in a variety of solid tumor cells, particularly carcinomas. Many of the tumors that contained TGF-a mRNA also expressed high levels of epidermal growth factor receptor mRNA. The concentration of TGF-a in the media of several tumor cell lines did not necessarily correlate with TGF-a mRNA levels, as a substantial fraction of TGF-a can remain cell associated. The levels of TGF-/3 mRNA in tumor cell lines and tumor specimens were variable, but higher in tumors than in the adjacent normal tissues.

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