Aim:To investigate the antiviral effect of compound of Curcuma zedoaria volatile oil and Hypericin perforatum extract liquid on H5N1 avian influenza virus (AIV) in MDCK cell line and non-AIV-immunized chickens.Methods:CPE inhibiton and MTT assay were used to study the inhibition of compound of Curcuma zedoaria volatile oil and Hypericin perforatum extract liquid,curcumenol and hypericin on H5N1 in MDCK,the retarding effect on H5N1 infection,the inhibition on the virus adsorption,and the therapeutic effect on the virus-infected cells.In addition,after one-week oral feeding of the liquid,non-AIV-immunized chickens were exposed to H5N1 AIV and the protecting effects of the liquid on the chickens were evaluated.The effect was also assessed after the chickens were simultaneously exposed to the liquid and and the attacking H5N1.The antiviral effects of oseltamivir and amantadine were also detected as the control groups.Results:In vitro results showed that compound of Curcuma zedoaria volatile oil and Hypericin perforatum extract liquid was effective via the mechanisms including virus killing,virus inhibiting,the prevention of the virus spread,and the treatment of the virus-related injury in MDCK.In vivo studies indicated that compound of Curcuma zedoaria volatile oil and Hypericin perforatum extract liquid prolonged the mean survival days of the chickens,showing significant preventive effect in the chickens.It was also proved that compound of Curcuma zedoaria volatile oil and Hypericin perforatum extract liquid was more effective than zedoary oil and Hypericum perforatum extract.Conclusion:Compound of Curcuma zedoaria volatile oil and Hypericin perforatum extract liquid is superior to zedoary oil and Hypericum perforatum in the treatment of H5N1 via the prevention of the virus infection and it might be very promising in the development into into effective anti-H5N1 virus drug.