Amperometric Detection and Quantification of 8‐Hydroxy‐2′‐deoxyguanosine (8‐OHdG) Using Dendrimer Modified Electrodes

8-Hydroxy-2′-deoxyguanosine (8-OHdG) detection by high performance liquid chromatography (HPLC) with amperometric detection was studied using a Au electrode modified with different dendrimer based thin films. Gold electrode is thiol-modified, forming self-assembled monolayers on which different generation PAMAM dendrimers with terminal functional groups COOH and NH2 have been attached using peptidic bonds. Results obtained in synthetic samples show low limits of detection and quantification for 8-OHdG (1.2×10−9 and 3.7×10−9 M respectively), with matrix interference elimination, thus avoiding sample pretreatment. Best results are obtained with electrodes modified with aliphatic amino thiols and 3.5 and 4.5 generation carboxylated dendrimers (Au/AET/DG3.5 and Au/AET/DG4.5), demonstrating that these materials constitute a good alternative for 8-OHdG determination in biological fluids.

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