Carbonic anhydrase VI exon 2 genetic polymorphism in Turkish subjects with low caries experience (preliminary study).

AIM The aim of this study was to investigate carbonic anhydrase (CA) VI exon 2 genetic polymorphism and its possible association with low caries experience in healthy young adults. PATIENTS AND METHODS Healthy young adults with caries or who where caries-free were recruited and unstimulated whole saliva and blood samples were taken. The number of decayed, missing and filled teeth (DMFT) and oral hygiene parameters were examined. Single nucleotide polymorphism (SNP) of CA VI gene exon 2 was determined by PCR and DNA sequencing. Salivary CA activity, buffering capacity and pH were also determined. RESULTS Two SNPs (dbSNP: 142460367 and 142460368), which are responsible for amino acid changes, were found. The frequencies of these SNPs were not significantly different between the caries-free group and the group with caries. There was no correlation between these SNPs and the salivary parameters. CONCLUSION Two SNPs found in young Turkish adults have no correlation with low caries prevalence.

[1]  L. S. Mofatto,et al.  Association of polymorphisms in the carbonic anhydrase 6 gene with salivary buffer capacity, dental plaque pH, and caries index in children aged 7–9 years , 2010, The Pharmacogenomics Journal.

[2]  M. Emi,et al.  Nucleotide variations in genes encoding carbonic anhydrase 8 and 10 associated with femoral bone mineral density in Japanese female with osteoporosis , 2009, Journal of Bone and Mineral Metabolism.

[3]  O. Sandgren,et al.  Carrier of R14W in carbonic anhydrase IV presents Bothnia dystrophy phenotype caused by two allelic mutations in RLBP1. , 2008, Investigative ophthalmology & visual science.

[4]  J. Leinonen.  Carbonic anhydrase isoenzyme VI: distribution, catalytic properties and biological significance , 2008 .

[5]  Si-wen Jiang,et al.  Expression pattern and polymorphism of three microsatellite markers in the porcine CA3 gene. , 2008, Genetics, selection, evolution : GSE.

[6]  C. Supuran,et al.  Carbonic anhydrase activators: the first activation study of the human secretory isoform VI with amino acids and amines. , 2007, Bioorganic & medicinal chemistry.

[7]  Y. Yura,et al.  A role of salivary carbonic anhydrase VI in dental plaque. , 2006, Archives of oral biology.

[8]  S. Parkkila,et al.  Carbonic anhydrases in normal gastrointestinal tract and gastrointestinal tumours. , 2005, World journal of gastroenterology.

[9]  C. Supuran,et al.  Carbonic anhydrases: current state of the art, therapeutic applications and future prospects. , 2004, Journal of enzyme inhibition and medicinal chemistry.

[10]  S. Parkkila,et al.  Salivary carbonic anhydrase VI and its relation to salivary flow rate and buffer capacity in pregnant and non-pregnant women. , 2003, Archives of oral biology.

[11]  W. Jiang,et al.  Structure of the carbonic anhydrase VI (CA6) gene: evidence for two distinct groups within the alpha-CA gene family. , 1999, The Biochemical journal.

[12]  S. Parkkila,et al.  Salivary carbonic anhydrase isoenzyme VI , 1999, The Journal of physiology.

[13]  Seppo Parkkila,et al.  A Low Concentration of Carbonic Anhydrase Isoenzyme VI in Whole Saliva Is Associated with Caries Prevalence , 1999, Caries Research.

[14]  D. Gupta,et al.  Structure of the carbonic anhydrase VI ( CA 6 ) gene : evidence for two distinct groups within the α-CA gene family , 1999 .

[15]  B. Martin,et al.  Gustin from human parotid saliva is carbonic anhydrase VI. , 1998, Biochemical and biophysical research communications.

[16]  W. Sly,et al.  Secretory carbonic anhydrase isoenzyme (CA VI) in human serum. , 1997, Clinical chemistry.

[17]  S. Parkkila,et al.  Salivary carbonic anhydrase VI concentration and its relation to basic characteristics of saliva in young men. , 1997, Acta Physiologica Scandinavica.

[18]  N. Bergenhem.  Chromatographic and electrophoretic methods related to the carbonic anhydrase isozymes. , 1996, Journal of chromatography. B, Biomedical applications.

[19]  S. Parkkila,et al.  Circadian periodicity in salivary carbonic anhydrase VI concentration. , 1995, Acta physiologica Scandinavica.

[20]  S. Parkkila,et al.  Immunohistochemical localization of carbonic anhydrase isoenzymes VI, II, and I in human parotid and submandibular glands. , 1990, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[21]  J. Edsall,et al.  Esterase activities of human carbonic anhydrases B and C. , 1967, The Journal of biological chemistry.

[22]  Y. Ericsson.  Clinical Investigations of the Salivary Buffering Action , 1959 .

[23]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.