Genetic variation in geographical populations of western and Mexican corn rootworm

Genetic variation in the nuclear rDNA ITS1 region of western corn rootworm, Diabrotica virgifera virgifera (WCR), and Mexican corn rootworm, D. v. zeae (MCR) was studied. Two sites were detected which differentiated WCR and MCR in the 642‐base sequence. Polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP) analysis of the first internal transcribed spacer region (ITS1) sequence revealed no variation within or among the twelve WCR and two MCR populations. PCR‐RFLP of 75% of the mitochondrial DNA genome detected one significant polymorphic site out of the approximately 190 restriction sizes observed in WCR. The polymorphism did not differentiate geographical populations of WCR and is not diagnostic for the subspecies. The low levels of variation observed in WCR suggests either high levels of gene flow or a recent geographical expansion from a relatively small base. Gene flow would facilitate the rapid spread of traits that could compromise control programmes, such as insecticide resistance or behavioural modifications. The minimal genetic differentiation between WCR and MCR raises questions about the evolutionary history of these subspecies and how the distinct phenotypes are maintained.

[1]  Allen L. Szalanski,et al.  Identification of Seed Gall Nematodes of Agronomic and Regulatory Concern with PCR-RFLP of ITS1. , 2001, Journal of nematology.

[2]  A. James,et al.  Genetic similarity among pheromone and voltinism races of Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) , 1999, Insect molecular biology.

[3]  R. Roehrdanz,et al.  Long Sections of Mitochondrial Dna Amplified from Fourteen Orders of Insects Using Conserved Polymerase Chain Reaction Primers , 1998 .

[4]  L. Chandler,et al.  Adult Susceptibility of Nebraska Western Corn Rootworm (Coleoptera: Chrysomelidae) Populations to Selected Insecticides , 1998 .

[5]  R. D. Peterson,et al.  Mitochondrial DNA Variation among Muscidifurax spp. (Hymenoptera: Pteromalidae), Pupal Parasitoids of Filth Flies (Diptera) , 1997 .

[6]  R. Giordano,et al.  The role of Wolbachia bacteria in reproductive incompatibilities and hybrid zones of Diabrotica beetles and Gryllus crickets. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[7]  D. Landis,et al.  Optimization of Soil Insecticide Rates for Managing Corn Rootworm (Coleoptera: Chrysomelidae) Larvae in the North Central United States , 1997 .

[8]  Allen L. Szalanski,et al.  The internal transcribed spacer region of belonolaimus (nemata: belonolaimidae). , 1997, Journal of nematology.

[9]  Allen L. Szalanski,et al.  Identification of Cyst Nematodes of Agronomic and Regulatory Concern with PCR-RFLP of ITS1. , 1997, Journal of nematology.

[10]  T. Powers,et al.  Molecular Differentiation of Alfalfa Weevil Strains (Coleoptera: Curculionidae) , 1996 .

[11]  R. D. Peterson,et al.  Population genetics and gene variation of stable fly populations (Diptera:Muscidae) in Nebraska. , 1996, Journal of medical entomology.

[12]  R. D. Peterson,et al.  Identification of screwworm species by polymerase chain reaction‐restriction fragment length polymorphism , 1996, Medical and veterinary entomology.

[13]  E. Krafsur.  Gene Flow between Univoltine and Semivoltine Northern Corn Rootworm (Coleoptera: Chrysomelidae) Populations , 1995 .

[14]  R. Roehrdanz.  Amplification of complete insect mitochondrial genome in two easy pieces , 1995, Insect molecular biology.

[15]  Juan D. López,et al.  Limited mitochondrial DNA polymorphism in North American populations of Heliothis virescens (Lepidoptera: Noctuidae) , 1994 .

[16]  B. Crespi,et al.  Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers , 1994 .

[17]  S. Bogdanowicz,et al.  Asian Gypsy Moths (Lepidoptera: Lymantriidae) in North America: Evidence from Molecular Data , 1993 .

[18]  B. Landry,et al.  A simple and rapid DNA microextraction method for plant, animal, and insect suitable for RAPD and other PCR analyses. , 1993, PCR methods and applications.

[19]  E. Krafsur,et al.  Gene Diversity in Natural Diabrotica barberi Smith & Lawrence Populations (Coleoptera: Chrysomelidae) , 1993 .

[20]  D. Wakarchuk,et al.  Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group. , 1992 .

[21]  A. Brower,et al.  MITOCHONDRIAL DNA VARIATION IN MONARCH BUTTERFLIES , 1991, Evolution; international journal of organic evolution.

[22]  E. Levine,et al.  Management of Diabroticite Rootworms in Corn , 1991 .

[23]  M. Degrugillier,et al.  Nonoccluded, cytoplasmic virus particles and rickettsia-like organisms in testes and spermathecae of , 1991 .

[24]  J. Tumlinson,et al.  Phenogram Based on Allozymes and Its Relationship to Classical Biosystematics and Pheromone Structure among Eleven Diabroticites (Coleoptera: Chrysomelidae) , 1989 .

[25]  R. F. Smith,et al.  Systematics of the virgifera species group of Diabrotica (Coleoptera: Chrysomelidae: Galerucinae). , 1987 .

[26]  J. L. Krysan.  Introduction: biology, distribution, and identification of pest Diabrotica , 1986 .

[27]  O. Johnson,et al.  Genetic Variation within and among Geographic Populations of Diabrotica barberi (Coleoptera: Chrysomelidae) , 1985 .

[28]  Ray F. Smith,et al.  Diabrotica barberi (Coleoptera: Chrysomelidae) elevated to species rank based on behavior, habitat choice, morphometrics, and geographical variation of color. , 1983 .

[29]  R. Metcalf.  Implications and Prognosis of Resistance to Insecticides , 1983 .

[30]  Ray F. Smith,et al.  A New Subspecies of Diabrotica virgifera (Coleoptera: Chrysomelidae): Description, Distribution, and Sexual Compatibility , 1980 .

[31]  J. L. Krysan,et al.  Inheritance of diapause intensity in Diabrotica virgifera , 1977 .

[32]  H. J. Ball,et al.  Differential Resistance of Corn Rootworms to Insecticides in Nehraska and Adjoining States , 1963 .