Yamadazyma cocois f.a., sp. nov., an ascomycetous yeast isolated from coconuts.

During studies on the endophytic yeast communities associated with fruits from Vietnam, three fermenting yeast strains were isolated from fruits of the coconut palm (Cocos nucifera). Phylogenetic analysis based on the sequences of the ITS regions and D1/D2 domains of the large subunit rRNA gene showed that these strains represented a single species of the Yamadazyma clade that was distinct from the other related species. The new species represented a basal branch of the clade formed by the Yamadazyma species i.e. Y. insecticola and Y. takamatsuzukensis. Based on the phylogenetic analysis and phenotypic characteristics, the studied strains were assigned to a novel species of the genus Yamadazyma, for which the name Yamadazyma cocois f.a., sp. nov. is proposed. The holotype is VCIM 4241, with the ex-type cultures VTCC 920004=VKM Y-3049=KBP Y-6091 code 17-68. The MycoBank number is MB 834435.

[1]  Kazutaka Katoh,et al.  MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization , 2017, Briefings Bioinform..

[2]  S. Limtong,et al.  Endophytic yeast diversity in leaf tissue of rice, corn and sugarcane cultivated in Thailand assessed by a culture-dependent approach. , 2018, Fungal biology.

[3]  S. Doty,et al.  Endophytic Yeasts: Biology, Ecology and Applications , 2018 .

[4]  Jacek Kominek,et al.  Genome sequence and physiological analysis of Yamadazyma laniorum f.a. sp. nov. and a reevaluation of the apocryphal xylose fermentation of its sister species, Candida tenuis , 2017, FEMS yeast research.

[5]  A. Glushakova,et al.  Endophytic yeasts in Malus domestica and Pyrus communis fruits under anthropogenic impact , 2017, Microbiology.

[6]  N. Čadež,et al.  Yeast habitats: Different but global , 2017 .

[7]  S. Casaregola,et al.  Yamadazyma barbieri f.a. sp. nov., an ascomycetous anamorphic yeast isolated from a Mid-Atlantic Ridge hydrothermal site (-2300 m) and marine coastal waters. , 2016, International journal of systematic and evolutionary microbiology.

[8]  D. Vu,et al.  DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation , 2016, Studies in mycology.

[9]  I. Chernov,et al.  Yeasts in Hevea brasiliensis latex , 2016, Microbiology.

[10]  S. Limtong,et al.  Yamadazyma endophytica f.a. sp. nov., an ascomycetous yeast species isolated from leaf tissue. , 2016, International journal of systematic and evolutionary microbiology.

[11]  J. Sugiyama,et al.  Yamadazyma kitorensis f.a., sp. nov. and Zygoascus biomembranicola f.a., sp. nov., novel yeasts from the stone chamber interior of the Kitora tumulus, and five novel combinations in Yamadazyma and Zygoascus for species of Candida. , 2016, International journal of systematic and evolutionary microbiology.

[12]  C. Rosa,et al.  Yamadazyma riverae sp. nov., a yeast species isolated from plant materials. , 2015, International journal of systematic and evolutionary microbiology.

[13]  Ying Li,et al.  Molecular Phylogeny and Taxonomy of Yamadazyma dushanensis f.a., sp. nov., a Cellobiose-Fermenting Yeast Species from China , 2015, Current Microbiology.

[14]  S. Limtong,et al.  Yamadazyma insecticola f.a., sp. nov. and Yamadazyma epiphylla f.a., sp. nov., two novel yeast species. , 2015, International journal of systematic and evolutionary microbiology.

[15]  S. Limtong,et al.  Wickerhamiella siamensis f.a., sp. nov., an endophytic and epiphytic yeast species isolated from sugar cane leaf. , 2014, International journal of systematic and evolutionary microbiology.

[16]  S. Limtong,et al.  Yamadazyma ubonensis f.a., sp. nov., a novel xylitol-producing yeast species isolated in Thailand , 2013, Antonie van Leeuwenhoek.

[17]  Koichiro Tamura,et al.  MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. , 2013, Molecular biology and evolution.

[18]  S. Doty Endophytic Yeasts: Biology and Applications , 2013 .

[19]  L. Corte,et al.  Yamadazyma terventina sp. nov., a yeast species of the Yamadazyma clade from Italian olive oils. , 2013, International journal of systematic and evolutionary microbiology.

[20]  H. Kawasaki,et al.  Yamadazyma siamensis sp. nov., Yamadazyma phyllophila sp. nov. and Yamadazyma paraphyllophila sp. nov., three novel yeast species isolated from phylloplane in Thailand and Taiwan , 2012, Antonie van Leeuwenhoek.

[21]  V. Robert,et al.  The value of the D1/D2 and internal transcribed spacers (ITS) domains for the identification of yeast species belonging to the genus Yamadazyma , 2011, Persoonia.

[22]  C. Kurtzman Yamadazyma Billon-Grand (1989) , 2011 .

[23]  C. Kurtzman,et al.  Candida Berkhout (1923) , 2011 .

[24]  C. Kurtzman,et al.  Methods for isolation, phenotypic characterization and maintenance of yeasts , 2011 .

[25]  A. V. Kachalkin,et al.  Endophytic yeast fungi in plant storage tissues , 2010, Biology Bulletin.

[26]  C. Kurtzman,et al.  Phylogenetic analysis of ascomycete yeasts that form coenzyme Q-9 and the proposal of the new genera Babjeviella, Meyerozyma, Millerozyma, Priceomyces, and Scheffersomyces , 2010, Mycoscience.

[27]  S. Rehner,et al.  A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs , 2005, Mycologia.

[28]  C. Kurtzman,et al.  Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences , 1998, Antonie van Leeuwenhoek.

[29]  B. Hall,et al.  Using RPB1 sequences to improve phylogenetic inference among mushrooms (Inocybe, Agaricales). , 2002, American journal of botany.

[30]  T. Bruns,et al.  ITS primers with enhanced specificity for basidiomycetes ‐ application to the identification of mycorrhizae and rusts , 1993, Molecular ecology.

[31]  G. Billon-Grand A new ascosporogenous yeast genus: Yamadazyma gen. nov. , 1989 .