Two-photon imaging with 80 MHz and 1-GHz repetition rate Ti:sapphire oscillators

We report on multiphoton optical imaging with a laser scanning microscope (TauMapTM, Jenlab GmbH) in combination with two different excitation fs-lasers: a 80 MHz Ti:sapphire oscillator generating spectrally tunable 100 fs pulses and a 1 GHz Ti:sapphire oscillator producing ultra broadband 6 fs pulses. While the ultra-broadband pulses enable simultaneous excitation of several different types of fluorophores due their large spectral width, the 100 fs pulses are spectrally more selective and require tuning the center wavelength to cover the same excitation range. The wavelength selectivity was confirmed in measurements with microspheres with absorption maxima in the green and blue spectral region. Furthermore, the potential of both lasers for imaging of human skin is evaluated.

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