In vitro micropropagation of local alfalfa Medicago sativa L. Gabsi.

In order to find an appropriate media enabling the in vitro regeneration of a whole plant of M.sativa L. Gabsi through micropropagation using leaf explants (cotyledon), we tested three different shoots developing media with three cytokinins: zeatin (Z), BA and TDZ on two different provenances of local M.sativa L. Gabsi 2 and 19. Secondly two different roots developing media, MS and MS / 2, were tested on the newly formed shoots. The medium Z, although it doesn’t always gave the best response rate, he gave the well developed shoots (up to 10 mm for M.sativa L. 19) with the lower vitrification rate (53, 12% for M.sativa L. 19). The MS medium doesn’t enable root formation on shoots grown on media Z, BA and TDZ while the medium MS / 2 allowed the formation of roots on shoots grown on the Z medium. The best combination of media allowing shoots and roots developing for both provenances of M.sativa L. Gabsi is that of Z medium with the MS / 2 medium.

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