Label-free photoelectrochemical strategy for hairpin DNA hybridization detection on titanium dioxide electrode

A new photoelectrochemical strategy for hairpin DNA hybridization was devised, in which TiO2 served as the anchor and signal transducer, and no label or redox couples were required. Once the hybridization between hairpin DNA probe and target DNA occurred, the photocurrent would decrease, utilizing which the sequence of the target DNA could be identified. The sequence specificity experiment showed that one or more mismatches of DNA bases could be discriminated. This photoelectrochemical method would be a potential tool in DNA hybridization detection due to its great advantages: label-free, high sensitivity, specific recognition, low cost, and easy fabrication.

[1]  Xiaobo Yu,et al.  Label-free electrochemical detection for aptamer-based array electrodes. , 2005, Analytical chemistry.

[2]  B. Giese.  Long-distance electron transfer through DNA. , 2002, Annual review of biochemistry.

[3]  M. Grätzel,et al.  Redox targeting of oligonucleotides anchored to nanocrystalline TiO2 films for DNA detection. , 2002, Chemphyschem : a European journal of chemical physics and physical chemistry.

[4]  L. Zhang,et al.  Pressurized electrochromatography coupled with electrospray ionization mass spectrometry for analysis of peptides and proteins. , 2004, Analytical chemistry.

[5]  T. Carell,et al.  Electrontransfer through DNA and metal-containing DNA. , 2003, Organic & biomolecular chemistry.

[6]  Ewa Heyduk,et al.  Molecular beacons for detecting DNA binding proteins: mechanism of action. , 2003, Analytical biochemistry.

[7]  Anders Hagfeldt,et al.  Investigation of influence of redox species on the interfacial energetics of a dye-sensitized nanoporous TiO2 solar cell , 1998 .

[8]  Tomoji Kawai,et al.  Control of electrical conduction in DNA using oxygen hole doping , 2002 .

[9]  Itamar Willner,et al.  Photoelectrochemistry with Controlled DNA-Cross-Linked CdS Nanoparticle Arrays This research is supported by The U.S.-Israel Binational Science Foundation. The Max Planck Research Award for International Cooperation (I.W.) is gratefully acknowledged. , 2001, Angewandte Chemie.

[10]  L. Murphy,et al.  Biosensors and bioelectrochemistry. , 2006, Current opinion in chemical biology.

[11]  P. Kamat,et al.  Improving the Photoelectrochemical Performance of Nanostructured TiO2 Films by Adsorption of Gold Nanoparticles , 2000 .

[12]  Chun-hui Huang,et al.  Photoelectric behavior of supercoiled DNA and its applications in molecular biology , 1998 .

[13]  Sanjay Tyagi,et al.  Molecular Beacons: Probes that Fluoresce upon Hybridization , 1996, Nature Biotechnology.

[14]  Norbert F. Scherer,et al.  Charge Transfer Across the Nanocrystalline-DNA Interface: Probing DNA Recognition , 2004 .

[15]  Rashid Bashir,et al.  Direct current electrical characterization of ds-DNA in nanogap junctions , 2005 .

[16]  T. G. Drummond,et al.  Electrochemical DNA sensors , 2003, Nature Biotechnology.