Structural and compositional characterization of the adhesive produced by reef building oysters.

Oysters have an impressive ability to overcome difficulties of life within the stressful intertidal zone. These shellfish produce an adhesive for attaching to each other and building protective reef communities. With their reefs often exceeding kilometers in length, oysters play a major role in balancing the health of coastal marine ecosystems. Few details are available to describe oyster adhesive composition or structure. Here several characterization methods were applied to describe the nature of this material. Microscopy studies indicated that the glue is comprised of organic fiber-like and sheet-like structures surrounded by an inorganic matrix. Phospholipids, cross-linking chemistry, and conjugated organics were found to differentiate this adhesive from the shell. Symbiosis in material synthesis could also be present, with oysters incorporating bacterial polysaccharides into their adhesive. Oyster glue shows that an organic-inorganic composite material can provide adhesion, a property especially important when constructing a marine ecosystem.

[1]  D. Jeong,et al.  Giant electrode effect on tunnelling electroresistance in ferroelectric tunnel junctions , 2014, Nature Communications.

[2]  A. Mount,et al.  Synergistic roles for lipids and proteins in the permanent adhesive of barnacle larvae , 2014, Nature Communications.

[3]  J. Waite,et al.  Sea star tenacity mediated by a protein that fragments, then aggregates , 2014, Proceedings of the National Academy of Sciences.

[4]  G. Jensen,et al.  Marine Tubeworm Metamorphosis Induced by Arrays of Bacterial Phage Tail–Like Structures , 2014, Science.

[5]  R. Stewart,et al.  Multipart copolyelectrolyte adhesive of the sandcastle worm, Phragmatopoma californica (Fewkes): catechol oxidase catalyzed curing through peptidyl-DOPA. , 2013, Biomacromolecules.

[6]  M. Meyers,et al.  Structural Biological Materials: Critical Mechanics-Materials Connections , 2013, Science.

[7]  P. Flammang,et al.  Unusual adhesive production system in the barnacle Lepas anatifera: An ultrastructural and histochemical investigation , 2012, Journal of morphology.

[8]  C. Spillmann,et al.  Barnacle Balanus amphitrite adheres by a stepwise cementing process. , 2012, Langmuir : the ACS journal of surfaces and colloids.

[9]  S. Weiner,et al.  Crystallization Pathways in Biomineralization , 2011 .

[10]  Russell J Stewart,et al.  Natural Underwater Adhesives. , 2011, Journal of polymer science. Part B, Polymer physics.

[11]  Jonathan J. Wilker,et al.  Oysters produce an organic-inorganic adhesive for intertidal reef construction. , 2010, Journal of the American Chemical Society.

[12]  J. Macdonald,et al.  Attachment of oysters to natural substrata by biologically induced marine carbonate cement , 2010 .

[13]  J. Macdonald,et al.  Alignment of crystallographic c-Axis throughout the four distinct microstructural layers of the oyster Crassostrea gigas , 2010 .

[14]  K. Wahl,et al.  Characterization of the adhesive plaque of the barnacle Balanus amphitrite: amyloid-like nanofibrils are a major component. , 2010, Langmuir : the ACS journal of surfaces and colloids.

[15]  R. M. A. Sullan,et al.  Insights into the composition, morphology, and formation of the calcareous shell of the serpulid Hydroides dianthus. , 2010, Journal of structural biology.

[16]  E. Hennebert,et al.  Polyphosphoprotein-Containing Marine Adhesives , 2009 .

[17]  R. M. A. Sullan,et al.  Nanoscale structures and mechanics of barnacle cement , 2009, Biofouling.

[18]  P. Singer,et al.  Inhibition of calcite precipitation by orthophosphate: Speciation and thermodynamic considerations , 2006 .

[19]  M. Kirby.  Fishing down the coast: historical expansion and collapse of oyster fisheries along continental margins. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[20]  J. Kere,et al.  Hemocyte-Mediated Shell Mineralization in the Eastern Oyster , 2004, Science.

[21]  J. Jackson.  What was natural in the coastal oceans? , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[22]  S. Harayama,et al.  Barnacle cement proteins. Importance of disulfide bonds in their insolubility. , 2000, The Journal of biological chemistry.

[23]  T. Quick,et al.  Differences in the Composition of Adhesive and Non-Adhesive Mucus From the Limpet Lottia limatula. , 1999, The Biological bulletin.

[24]  E. Harper.  Attachment of mature oysters (Saccostrea cucullata ) to natural substrata , 1997 .

[25]  K. Yamaguchi.  Shell structure and behaviour related to cementation in oysters , 1994 .

[26]  E. Harper.  POST-LARVAL CEMENTATION IN THE OSTREIDAE AND ITS IMPLICATIONS FOR OTHER CEMENTING BIVALVIA , 1992 .

[27]  R. Colwell,et al.  Characterization of a Marine Bacterium Associated with Crassostrea virginica (the Eastern Oyster) , 1985, Applied and environmental microbiology.

[28]  S. Weiner,et al.  Soluble protein of the organic matrix of mollusk shells: a potential template for shell formation , 1975, Science.

[29]  G. Walker.  The Biochemical Composition of the Cement of two Barnacle Species, Balanus Hameri and Balanus Crenatus , 1972, Journal of the Marine Biological Association of the United Kingdom.

[30]  K. Simkiss,et al.  PHOSPHATES AS CRYSTAL POISONS OF CALCIFICATION , 1964, Biological reviews of the Cambridge Philosophical Society.

[31]  G. Vermeij.  The oyster enigma variations: a hypothesis of microbial calcification , 2014, Paleobiology.

[32]  J. Waite,et al.  Chemical Subtleties of Mussel and Polychaete Holdfasts , 2006 .

[33]  K. Wilbur.  CHAPTER 8 – Shell Formation and Regeneration , 1964 .