An estimation of tissue damage and thermal history in the cryolesion.

Abstract The cooling and thawing rates at fixed sites within an expanding ice ball have been recorded using a temperature probe containing six integral thermocouples. The cell changes relevant to these sites have been followed through the 48 hr postfreeze-thaw period by light and electron microscopy. The results suggest that cooling rates that are compatible with cell survival in cell suspension are associated with uniform tissue cellular death in an organized tissue.

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