CYTOTOXICITY AND ANTIMICROBIAL ACTIVITY OF SATUREJA KITAIBELII WIERZB. EX HEUFF (LAMIACEAE)

Methanolic extract of Satureja kitaibelii Wierzb. ex Heuff. (Lamiaceae) and its major compond rosmarinic acid possessed strong antimicrobial activity against tested bacteria and fungi, and antitumour activity against malignant cells, but without antiproliferative effect on healthy cell line MRC5. Minimal inhibitory concentration (MIC) of methanolic extract was ranged between 0.0125-1.25 mg/ml, while minimal bactericidal concentration (MBC) was of 0.2-5.0 mg/ml and fungicidal (MFC) was 0.15-2.5 mg/ml. The rosmarinic acid exhibited better antimicrobial activity then methanol extract. The most sensitive species to rosmarinic acid were Bacillus cereus and Candida kruzei, while Micrococcus flavus, Trichoderma viride and Aspergillus niger were the most resistant. The methanol extract exhibited strong activity against Fem-x human malignant melanoma cells with an IC50 39.66±2.71 µg/ml, and moderate activity against other cancer cell lines (IC50 from 138.06±0.16 µg/ml against estrogen-dependant breast cancer cell lines MDA-MB-361 to 173.15±0.02 against a human epithelial cervical cancer cells HeLa). Rosmarinic acid arrested G2/M phase cell cycle in Fem-x cells, against which both methanol extract and rosmarinic acid possessed the best cytotoxic activity. (Received March 10, 2013; Accepted May 23, 2013)

[1]  Yi‐Chen Chen,et al.  Antioxidant, anti-proliferative and cyclooxygenase-2 inhibitory activities of ethanolic extracts from lemon balm (Melissa officinalis L.) leaves , 2012 .

[2]  Tai-Long Pan,et al.  Inhibitory effects of Scutellaria baicalensis extract on hepatic stellate cells through inducing G2/M cell cycle arrest and activating ERK-dependent apoptosis via Bax and caspase pathway. , 2012, Journal of ethnopharmacology.

[3]  N. Kovačević,et al.  Composition of Satureja kitaibelii Essential Oil and its Antimicrobial Activity , 2011, Natural product communications.

[4]  D. Radnović,et al.  Chemical Composition and Antimicrobial activity of Satureja kitaibelii Essential Oil against Pathogenic Microbial Strains , 2011, Natural product communications.

[5]  M. Nunes,et al.  Antioxidant and antimicrobial activity of Satureja montana L. extracts. , 2011, Journal of the science of food and agriculture.

[6]  J. Hadian,et al.  Phytochemical and Morphological Characterization of Satureja khuzistanica Jamzad Populations from Iran , 2011, Chemistry & biodiversity.

[7]  S. Salawu,et al.  Antimicrobial activities of phenolic containing extracts of some tropical vegetables , 2011 .

[8]  Ligang Zhou,et al.  Diterpenoid Tanshinones and Phenolic Acids from Cultured Hairy Roots of Salvia miltiorrhiza Bunge and Their Antimicrobial Activities , 2011, Molecules.

[9]  Hayriye Cetin‐Karaca,et al.  EVALUATION OF NATURAL ANTIMICROBIAL PHENOLIC COMPOUNDS AGAINST FOODBORNE PATHOGENS , 2011 .

[10]  B. Sadeghi-Nejad,et al.  Antifungal activity of Satureja khuzestanica (Jamzad) leaves extracts , 2010 .

[11]  J. Hadian,et al.  Variability of morphological and phytochemical characteristics among Satureja hortensis L. accessions of Iran , 2010 .

[12]  M. Abdollahi,et al.  An update on pharmacology of Satureja species; from antioxidant, antimicrobial, antidiabetes and anti-hyperlipidemic to reproductive stimulation , 2010 .

[13]  S. Saeidnia,et al.  PHYTOCHEMISTRY AND ANTIMICROBIAL COMPOUNDS OF HYMENOCRATER CALYCINUS , 2009 .

[14]  M. Ateş,et al.  Characterization of the phenolic composition and antimicrobial activities of Turkish medicinal plants , 2009 .

[15]  A. Sokmen,et al.  Production and optimisation of rosmarinic acid by Satureja hortensis L. callus cultures , 2007, Natural product research.

[16]  G. Ćetković,et al.  Antioxidant Potential, Lipid Peroxidation Inhibition and Antimicrobial Activities of Satureja montana L. subsp. kitaibelii Extracts , 2007, International Journal of Molecular Sciences.

[17]  T. Yoshikawa,et al.  Absorption, metabolism, degradation and urinary excretion of rosmarinic acid after intake of Perilla frutescens extract in humans , 2005, European journal of nutrition.

[18]  G. Bogdanovic,et al.  Antioxidative and antiproliferative effects of Satureja montana L. extracts. , 2004, Journal of B.U.ON. : official journal of the Balkan Union of Oncology.

[19]  H. J. Dorman,et al.  An Improved On-Line HPLC-DPPH Method for the Screening of Free Radical Scavenging Compounds in Water Extracts of Lamiaceae Plants , 2003, Chemistry of Natural Compounds.

[20]  M. Simmonds,et al.  Rosmarinic acid. , 2003, Phytochemistry.

[21]  M. Tsimidou,et al.  Antioxidant activities and phenolic composition of extracts from Greek oregano, Greek sage, and summer savory. , 2002, Journal of agricultural and food chemistry.

[22]  E. Tsankova,et al.  The Essential Oils of Satureja montana ssp. kitaibelii Wierzb. and Satureja pilosa var. pilosa Velen from Bulgaria , 2002 .

[23]  J. Harborne,et al.  Acacetin glycosides as taxonomic markers in Calamintha and Micromeria. , 2001, Phytochemistry.

[24]  S. Milosavljevic,et al.  Variability of essential oils of Satureja montana L. and Satureja kitaibelii wierzb. ex Heuff. from the central part of the balkan peninsula. , 2001, Phytochemistry.

[25]  G. Blunden,et al.  Comparative studies of the rosmarinic and caffeic acid contents of Lamiaceae species , 1999 .

[26]  S. Pepeljnjak,et al.  Antimicrobial activity of the ethanolic extract of Satureja montana ssp. montana , 1999 .

[27]  S. Dagher,et al.  Antifungal Activity of the Essential Oil of Origanum syriacum L. , 1995, Journal of food protection.

[28]  M. Ohno,et al.  Rapid colorimetric assay for the quantification of leukemia inhibitory factor (LIF) and interleukin-6 (IL-6). , 1991, Journal of immunological methods.

[29]  H. Hänel,et al.  A More Sophisticated Method of Determining the Fungicidal Effect of Water‐Insoluble Preparations with a Cell Harvester, Using Miconazole as an Example./Eine verbesserte Methode zur Bestimmung der Fungizidie von wasserunlöslichen Präparaten mit Hilfe eines Zellerntegerätes am Beispiel von Miconazol , 1988 .

[30]  P. Živanović,et al.  The qualitative composition of the essential oil in species genus Satureja L. (Lamiaceae) distribution in Yugoslavia , 1987 .

[31]  T. Mosmann.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. , 1983, Journal of immunological methods.