Clinically acceptable restorations may be a hotbed for cariogenic microbes.

OBJECTIVE The aim of this study was to investigate the cross-sectional association of dental restorations with salivary cariogenic pathogens among the elderly to establish effective parameters of caries risk for this population. MATERIALS AND METHODS Stimulated whole saliva was collected from 289 community-dwelling older adults (66.2 ± 3.9 years old) who had 20 or more teeth. Salivary levels of three cariogenic bacteria (Streptococcus mutans, Streptococcus sobrinus and lactobacilli) were estimated using quantitative polymerase chain reaction (real-time PCR) method. RESULTS The mean number of residual teeth was 26.4, and restored teeth with crowns, inlays and composite resin were 7.35, 3.88 and 0.68, respectively. The number of crowns correlated positively with salivary S. mutans, S. sobrinus and lactobacilli. Multiple linear regression analyses showed that the number of restored teeth with crowns was independently associated with salivary S. mutans, S. sobrinus and lactobacilli after controlling for age, gender, number of residual teeth and salivary flow rate. Salivary flow rate was independently associated with salivary S. mutans and lactobacilli. CONCLUSION The number of crowns had an association with salivary levels of cariogenic bacteria, suggesting that this parameter may be a caries risk indicator for the elderly population.

[1]  K. Ikebe,et al.  Association of salivary Streptococcus mutans levels determined by rapid detection system using monoclonal antibodies with prevalence of root surface caries. , 2008, American journal of dentistry.

[2]  J. Loyola-Rodríguez,et al.  Distribution of Streptococcus mutans and Streptococcus sobrinus in saliva of Mexican preschool caries-free and caries-active children by microbial and molecular (PCR) assays. , 2007, The Journal of clinical pediatric dentistry.

[3]  Y. Nomura,et al.  Correlation of cariogenic bacteria and dental caries in adults. , 2006, Journal of oral science.

[4]  S. Fure.  Ten-year cross-sectional and incidence study of coronal and root caries and some related factors in elderly Swedish individuals. , 2004, Gerodontology.

[5]  T. Koga,et al.  Development of a 5′ Nuclease-Based Real-Time PCR Assay for Quantitative Detection of Cariogenic Dental Pathogens Streptococcus mutans and Streptococcus sobrinus , 2003, Journal of Clinical Microbiology.

[6]  J M Tanzer,et al.  The microbiology of primary dental caries in humans. , 2001, Journal of dental education.

[7]  C. Emilson,et al.  Root Caries and Some Related Factors in 88-Year-Old Carriers and Non-Carriers of Streptococcus sobrinus in Saliva , 1998, Caries Research.

[8]  W. H. van Palenstein Helderman,et al.  Cariogenicity Depends More on Diet than the Prevailing Mutans Streptococcal Species , 1996, Journal of dental research.

[9]  E. Kidd,et al.  Marginal Ditching and Staining as a Predictor of Secondary Caries Around Amalgam Restorations: A Clinical and Microbiological Study , 1995, Journal of dental research.

[10]  D. Birkhed,et al.  Acid production by human strains of Streptococcus mutans and Streptococcus sobrinus. , 1995, Caries research.

[11]  M. E. Davis,et al.  A microbiological study of recurrent dentinal caries. , 1994, Caries research.

[12]  B. Köhler,et al.  Influence of salivary levels of mutans streptococci on colonization of crown margins: a longitudinal study. , 1993, The Journal of prosthetic dentistry.

[13]  E. Isogai,et al.  Close association between Streptococcus sobrinus in the saliva of young children and smooth-surface caries increment. , 1993, Caries research.

[14]  B. Köhler,et al.  Salivary levels of mutans streptococci and lactobacilli in dentate 80- and 85-year-old Swedish men and women. , 1991, Community dentistry and oral epidemiology.

[15]  J. T. ten Cate,et al.  Differences in cariogenicity between fresh isolates of Streptococcus sobrinus and Streptococcus mutans. , 1991, Caries research.

[16]  S. Fure,et al.  Root surface caries and associated factors. , 1990, Scandinavian journal of dental research.

[17]  S. Fure,et al.  Salivary conditions and cariogenic microorganisms in 55, 65, and 75-year-old Swedish individuals. , 1990, Scandinavian journal of dental research.

[18]  T. Johansson,et al.  Presence of mutans streptococci and various types of lactobacilli in interdental spaces related to development of proximal carious lesions. , 1989, Scandinavian journal of dental research.

[19]  J. de Graaff,et al.  Acidogenesis by oral streptococci at different pH values. , 1989, Caries research.

[20]  I. Thorselius,et al.  Prevalence of mutans streptococci and lactobacilli in elderly Swedish individuals. , 1988, Scandinavian journal of dental research.

[21]  S. Bjarnason,et al.  Mutans streptococci, lactobacilli and caries prevalence in 11- and 12-year-old Icelandic children. , 1987, Community dentistry and oral epidemiology.

[22]  C. Emilson,et al.  Support for and implications of the specific plaque hypothesis. , 1985, Scandinavian journal of dental research.

[23]  H. Gröndahl,et al.  The More Streptococcus mutans, the More Caries on Approximal Surfaces , 1985, Journal of dental research.

[24]  B. Burt,et al.  Longitudinal investigation of bacteriology of human fissure decay: epidemiological studies in molars shortly after eruption , 1984, Infection and immunity.

[25]  D. Bratthall,et al.  Streptococcus mutans in saliva: intraindividual variations and relation to the number of colonized sites. , 1984, Acta odontologica Scandinavica.

[26]  C. Crossner.  Salivary lactobacillus counts in the prediction of caries activity. , 1981, Community dentistry and oral epidemiology.

[27]  J. van Houte,et al.  Relationship Between the Concentration of Bacteria in Saliva and the Colonization of Teeth in Humans , 1974, Infection and immunity.