MORPHOLOGICAL, CULTURAL AND PHYSIOLOGICAL STUDIES ON Slerotinia sclerotiorum CAUSING STALK ROT OF CAULIFLOWER

Morphological, cultural and physiological of Sclerotinia sclerotiorum were studied on solid and liquid media at Vegetable Pathology Laboratory, Department of Mycology and Plant Pathology, Dr. Y.S. Parmar University of Horticulture and Forestry, Himachal Pradesh, India. The fungus produced aerial mycelium, which was hyaline consisting of closely septate hyphae and measured 2.0-9.4 µm. Micro- conidia were produced on conidiophores of the vegetative mycelium and measured 1.5-3.5 µm. Sclerotia were black in colour, round to irregular and measured 2-15 x 1.5-7 mm. Apothecia were round or globate type with diameter ranging from 2-9 mm. The asci were hyaline and cylindrical and measured 91-165 x 4.9-8.5 µm. Ascospores were elliptical and ranged from 6.5-13 x 4.2-6.6 µm. The fungus was found to grow best on potato dextrose agar medium and Richard solution. Optimum temperature and pH for the growth of the fungus were 20-25 o C and pH 5.0, respectively.

[1]  H. J. Willetts,et al.  The biology ofSclerotinia sclerotiorum,S. trifoliorum, andS. minor with emphasis on specific nomenclature , 1980, The Botanical Review.

[2]  G. Boland,et al.  Sclerotinia Rot of Carrot: An Example of Phenological Adaptation and Bicyclic Development by Sclerotinia Sclerotiorum. , 2003, Plant disease.

[3]  James B. Anderson,et al.  Restriction fragment length polymorphisms in nuclear and mitochondrial DNA of Sclerotinia species , 1988 .

[4]  M. A. Khan,et al.  Cultural control of Sclerotinia stalk rot of cauliflower. , 1983 .

[5]  R. Sharma STALK ROT OF CAULIFLOWER CAUSED BY SCLEROTINIA SCLEROTIORUM(Lib.) DE BARY , 1979 .

[6]  R. G. Grogan SclerotiniaSpecies: Summary and Comments on Needed Research , 1979 .

[7]  D. L. Tourneau Morphology, Cytology, and Physiology ofSclerotiniaSpecies in Culture , 1979 .

[8]  Mohammad A. U. Khan STUDIES ON STALK ROT OF CAULIFLOWER CAUSED BY SCLEROTINIA SCLEROTIORUM(lIB.)DE BARY , 1977 .

[9]  D. S. Kaith FURTHER STUDIES ON STALK ROT OF CAULIFLOWER CAUSED BY SCLEROTINIA SCLEROTIORUM(LIB.) DE BARY , 1977 .

[10]  G. Abawi,et al.  Source of primary inoculum and effects of temperature and moisture on infection of beans by Whetzelinia sclerotiorum. , 1975 .

[11]  N. Colotelo A scanning electron microscope study of developing sclerotia of Sclerotinia sclerotiorum , 1974 .

[12]  K. S. Bedi Temperature in relation to the formation of apothecia ofSclerotinia sclerotiorum (Lib.) de Bary , 1962, Proceedings / Indian Academy of Sciences.

[13]  L. H. Purdy Factors affecting apothecial production by Sclerotinia sclerotiorum. , 1956 .

[14]  D. M. Coe Variations in Single Ascospore Isolates of Sclerotinia Sclerotiorum , 1944 .

[15]  D. Green Diseases of Vegetable Crops , 1940, Nature.