Development of MoSe2 Nano-Urchins as a Sensing Platform for a Selective Bio-Capturing of Escherichia coli Shiga Toxin DNA

The present study was aimed to develop “fluorine doped” tin oxide glass electrode with a MoSe2 nano-urchin based electrochemical biosensor for detection of Escherichia coli Shiga toxin DNA. The study comprises two conductive electrodes, and the working electrodes were drop deposited using MoSe2 nano-urchin, and DNA sequences specific to Shiga toxin Escherichia coli. Morphological characterizations were performed using Fourier transforms infrared spectrophotometer; X-ray diffraction technique and scanning electron microscopy. All measurements were done using methylene blue as an electrochemical indicator. The proposed electrochemical geno-sensor showed good linear detection range of 1 fM–100 µM with a low detection limit of 1 fM where the current response increased linearly with Escherichia coli Shiga toxin dsDNA concentration with R2 = 0.99. Additionally, the real sample was spiked with the dsDNA that shows insignificant interference. The results revealed that the developed sensing platform significantly improved the sensitivity and can provide a promising platform for effective detection of biomolecules using minute samples due to its stability and sensitivity.

[1]  M. Xue,et al.  Hydrothermal synthesis and tribological properties of MoSe2 nanoflowers , 2015 .

[2]  C. A. Baker Shiga Toxin-Producing Escherichia coli (STEC) Detection Strategies with Formalin-Fixed STEC Cells , 2015 .

[3]  Dmitri Ivnitski,et al.  Biosensors for detection of pathogenic bacteria , 1999 .

[4]  Eirini Christaki New technologies in predicting, preventing and controlling emerging infectious diseases , 2015, Virulence.

[5]  Jagriti Narang,et al.  Ultrasensitive electrochemical immuno-sensing platform based on gold nanoparticles triggering chlorpyrifos detection in fruits and vegetables. , 2018, Biosensors & bioelectronics.

[6]  Liu Yang,et al.  Dual signal-amplification electrochemical detection of DNA sequence based on molybdenum selenide nanorod and hybridization chain reaction , 2016 .

[7]  L. Cebotaru,et al.  Enterohemorrhagic Escherichia coli infection stimulates Shiga toxin 1 macropinocytosis and transcytosis across intestinal epithelial cells. , 2011, American journal of physiology. Cell physiology.

[8]  Steve Tung,et al.  Development and Applications of Portable Biosensors , 2015, Journal of laboratory automation.

[9]  C. Pundir,et al.  Portable bioactive paper based genosensor incorporated with Zn-Ag nanoblooms for herpes detection at the point-of-care. , 2018, International journal of biological macromolecules.

[10]  James C. Paton,et al.  Pathogenesis and Diagnosis of Shiga Toxin-Producing Escherichia coli Infections , 1998, Clinical Microbiology Reviews.

[11]  S. Dong,et al.  Nucleic Acid Biosensors: Recent Advances and Perspectives. , 2017, Analytical chemistry.

[12]  E. Paleček Electrochemical techniques , 1978, Nature.

[13]  A. S. Bawa,et al.  Genetically modified foods: safety, risks and public concerns—a review , 2013, Journal of Food Science and Technology.

[14]  Vinay Gupta,et al.  Flower-like ZnO nanostructure based electrochemical DNA biosensor for bacterial meningitis detection. , 2014, Biosensors & bioelectronics.

[15]  Kok-Gan Chan,et al.  Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations , 2015, Front. Microbiol..

[16]  Hongwei Zhu,et al.  Two-dimensional MoS2: Properties, preparation, and applications , 2015 .

[17]  Evangelyn C. Alocilja,et al.  Electrochemical Biosensor for Rapid and Sensitive Detection of Magnetically Extracted Bacterial Pathogens , 2012, Biosensors.

[18]  Kavitha Pathakoti,et al.  Nanostructures: Current uses and future applications in food science , 2017, Journal of food and drug analysis.

[19]  V. Adam,et al.  G-Quadruplexes as Sensing Probes , 2013, Molecules.

[20]  Y. Choong,et al.  Phage Display‐Derived Antibodies: Application of Recombinant Antibodies for Diagnostics , 2016 .

[21]  Sanying Wang,et al.  Rapid Detection of Shigella Species in Environmental Sewage by an Immunocapture PCR with Universal Primers , 2002, Applied and Environmental Microbiology.

[22]  Deepika Singh,et al.  Nucleic Acid Based Biosensors for Clinical Applications , 2013 .

[23]  Joseph C Liao,et al.  Advances and challenges in biosensor-based diagnosis of infectious diseases , 2014, Expert review of molecular diagnostics.

[24]  Feng Li,et al.  Development of an electrochemical DNA biosensor with a high sensitivity of fM by dendritic gold nanostructure modified electrode. , 2011, Biosensors & bioelectronics.

[25]  Mohammed Zourob,et al.  DNA-Based Nanobiosensors as an Emerging Platform for Detection of Disease , 2015, Sensors.

[26]  K. Sandvig,et al.  Entry of ricin and Shiga toxin into cells: molecular mechanisms and medical perspectives , 2000, The EMBO journal.

[27]  Chengzhou Zhu,et al.  Electrochemical Sensors and Biosensors Based on Nanomaterials and Nanostructures , 2014, Analytical chemistry.