A new europium chelate for protein labelling and time-resolved fluorometric applications.

Synthetic procedures are presented for a new chelator that forms stable and highly fluorescent complexes with Eu3+. This chelator, 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) is synthesized in a high-yield three-step procedure. BCPDA can be covalently incorporated into proteins under relatively mild conditions, and when complexed with Eu3+ forms a fluorescent product that has a lifetime in the range of 0.4 to 0.7 ms. Thus, it is useful for time-resolved fluorescence immunoassay applications.

[1]  B. Karger,et al.  Dansylation of amino acids for high-performance liquid chromatography analysis. , 1981, Analytical biochemistry.

[2]  H. Déchaud,et al.  Laser-excited immunofluorometric assay of prolactin, with use of antibodies coupled to lanthanide-labeled diethylenetriaminepentaacetic acid. , 1986, Clinical chemistry.

[3]  T. L. Miller,et al.  Spectrofluorometric determination of calcium and lanthanide elements in dilute solution , 1982 .

[4]  I Hemmilä,et al.  Fluoroimmunoassay: present status and key problems. , 1979, Clinical chemistry.

[5]  M. Mcentegart,et al.  Fluorescent protein tracers; a trial of new fluorochromes and the development of an alternative to fluorescein. , 1958, Immunology.

[6]  E Soini,et al.  Time-resolved fluorometer for lanthanide chelates--a new generation of nonisotopic immunoassays. , 1983, Clinical chemistry.

[7]  A. Sinha.  FLUORESCENCE AND LASER ACTION IN RARE EARTH CHELATES , 1971 .

[8]  I Hemmilä,et al.  Fluoroimmunoassays and immunofluorometric assays. , 1985, Clinical chemistry.

[9]  Eleftherios P. Diamandis,et al.  Immunoassays with time-resolved fluorescence spectroscopy: Principles and applications , 1988 .

[10]  R. Zare,et al.  Direct measurement of antigens in serum by time-resolved fluoroimmunoassay. , 1985, Clinical chemistry.

[11]  R. Ekins,et al.  The fluorometric determination of europium ion concentration as used in time-resolved fluoroimmunoassay. , 1985, Analytical biochemistry.

[12]  G. Newkome,et al.  α-Methyl functionalization of electron-poor heterocycles: 2,9-bis(chloromethyl)-1,10-phenanthroline. Synthesis of a [3.3]cyclophane containing the 1,10-phenanthrolino moiety , 1983 .

[13]  V. Koistinen,et al.  Detection of hepatitis B surface antigen using time-resolved fluoroimmunoassay , 1983, Nature.