Hydrophilic, bright CuInS2 quantum dots as Cd-free fluorescent labels in quantitative immunoassay.

We report on the synthesis of core-shell CuInS2/ZnS quantum dots (QDs) in organic solution, their encapsulation with a PEG-containing amphiphilic polymer, and the application of the resulting water-soluble QDs as fluorescent label in quantitative immunoassay. By optimizing the methods for core synthesis and shell growth, CuInS2/ZnS QDs were obtained with a quantum yield of 50% on average after hydrophilization. After conjugation with an aflatoxin B1-protein derivative, the obtained QDs were used as fluorescent labels in microplate immunoassay for the quantitative determination of the mycotoxin aflatoxin B1. QDs-based immunoassay showed higher sensitivity compared to enzyme-based immunoassay.

[1]  Zeger Hens,et al.  Polymer-coated fluorescent CdSe-based quantum dots for application in immunoassay. , 2014, Biosensors & bioelectronics.

[2]  Peiwu Li,et al.  Monoclonal antibody-quantum dots CdTe conjugate-based fluoroimmunoassay for the determination of aflatoxin B1 in peanuts. , 2014, Food chemistry.

[3]  R. Niessner,et al.  Quantum dot loaded liposomes as fluorescent labels for immunoassay. , 2013, Analytical chemistry.

[4]  M. Tabatabaei,et al.  Highly sensitive FRET-based fluorescence immunoassay for aflatoxin B1 using cadmium telluride quantum dots , 2013, Microchimica Acta.

[5]  N. J. Johnson,et al.  Long-Term Colloidal Stability and Photoluminescence Retention of Lead-Based Quantum Dots in Saline Buffers and Biological Media through Surface Modification , 2013 .

[6]  Wei-Ting Huang,et al.  Gadolinium-based CuInS2/ZnS nanoprobe for dual-modality magnetic resonance/optical imaging. , 2013, ACS applied materials & interfaces.

[7]  Haizheng Zhong,et al.  Integration of CuInS2-based nanocrystals for high efficiency and high colour rendering white light-emitting diodes. , 2013, Nanoscale.

[8]  S. Saeger,et al.  Immunochemical approach for zearalenone-4-glucoside determination. , 2013, Talanta.

[9]  Z. Jakubek,et al.  Low-temperature approach to highly emissive copper indium sulfide colloidal nanocrystals and their bioimaging applications. , 2013, ACS applied materials & interfaces.

[10]  Weisheng Guo,et al.  Synthesis of Zn-Cu-In-S/ZnS Core/Shell Quantum Dots with Inhibited Blue-Shift Photoluminescence and Applications for Tumor Targeted Bioimaging , 2013, Theranostics.

[11]  Hicham A. Chibli,et al.  InP/ZnS as a safer alternative to CdSe/ZnS core/shell quantum dots: in vitro and in vivo toxicity assessment. , 2013, Nanoscale.

[12]  C. Murray,et al.  Coating Evaluation and Purification of Monodisperse, Water-Soluble, Magnetic Nanoparticles Using Sucrose Density Gradient Ultracentrifugation. , 2012, Chemistry of materials : a publication of the American Chemical Society.

[13]  Haizheng Zhong,et al.  Tuning the Luminescence Properties of Colloidal I-III-VI Semiconductor Nanocrystals for Optoelectronics and Biotechnology Applications. , 2012, The journal of physical chemistry letters.

[14]  Qin Zhifeng,et al.  Facile synthesis of high-quality CuInZnxS2+x core/shell nanocrystals and their application for detection of C-reactive protein , 2012 .

[15]  S. Achilefu,et al.  High-Quality CuInS2/ZnS Quantum Dots for In vitro and In vivo Bioimaging , 2012 .

[16]  C. D'Andrea,et al.  Strongly Fluorescent Quaternary Cu-In-Zn-S Nanocrystals Prepared from Cu1-xInS2 Nanocrystals by Partial Cation Exchange , 2012 .

[17]  Zhan'ao Tan,et al.  Highly Emissive and Color‐Tunable CuInS2‐Based Colloidal Semiconductor Nanocrystals: Off‐Stoichiometry Effects and Improved Electroluminescence Performance , 2012 .

[18]  Jia-Yaw Chang,et al.  Strategies for photoluminescence enhancement of AgInS2 quantum dots and their application as bioimaging probes , 2012 .

[19]  S. Saeger,et al.  Quantum dot based rapid tests for zearalenone detection , 2012, Analytical and Bioanalytical Chemistry.

[20]  D. Pang,et al.  Tapping the potential of quantum dots for personalized oncology: current status and future perspectives. , 2012, Nanomedicine.

[21]  Weiling Fu,et al.  Sensitive and rapid quantification of C-reactive protein using quantum dot-labeled microplate immunoassay , 2012, Journal of Translational Medicine.

[22]  Heesun Yang,et al.  Noninjection, one-pot synthesis of Cu-deficient CuInS2/ZnS core/shell quantum dots and their fluorescent properties. , 2011, Journal of colloid and interface science.

[23]  R. Niessner,et al.  A comparison of horseradish peroxidase, gold nanoparticles and qantum dots as labels in non-instrumental gel-based immunoassay , 2011 .

[24]  Igor L. Medintz,et al.  Optimizing Two-Color Semiconductor Nanocrystal Immunoassays in Single Well Microtiter Plate Formats , 2011, Sensors.

[25]  Jaehyun Park,et al.  CuInS2/ZnS core/shell quantum dots by cation exchange and their blue-shifted photoluminescence , 2011 .

[26]  V. Klimov,et al.  Efficient synthesis of highly luminescent copper indium sulfide-based core/shell nanocrystals with surprisingly long-lived emission. , 2011, Journal of the American Chemical Society.

[27]  Dmitri I Svergun,et al.  Hydrophilization of Magnetic Nanoparticles with Modified Alternating Copolymers. Part 1: The Influence of the Grafting. , 2010, The journal of physical chemistry. C, Nanomaterials and interfaces.

[28]  Yuehe Lin,et al.  CdSe/ZnS quantum dots based electrochemical immunoassay for the detection of phosphorylated bovine serum albumin. , 2010, Biosensors & bioelectronics.

[29]  T. Mirkovic,et al.  Noninjection gram-scale synthesis of monodisperse pyramidal CuInS2 nanocrystals and their size-dependent properties. , 2010, ACS nano.

[30]  Shuming Nie,et al.  Molecular mapping of tumor heterogeneity on clinical tissue specimens with multiplexed quantum dots. , 2010, ACS nano.

[31]  Hong Ding,et al.  Synthesis of ternary CuInS(2)/ZnS quantum dot bioconjugates and their applications for targeted cancer bioimaging. , 2010, Integrative biology : quantitative biosciences from nano to macro.

[32]  D. Deforce,et al.  Application of a new anti-zearalenone monoclonal antibody in different immunoassay formats , 2009, Analytical and bioanalytical chemistry.

[33]  Liang Li,et al.  Highly Luminescent CuInS2/ZnS Core/Shell Nanocrystals: Cadmium-Free Quantum Dots for In Vivo Imaging , 2009 .

[34]  P. Mulvaney,et al.  The preparation of colloidally stable, water-soluble, biocompatible, semiconductor nanocrystals with a small hydrodynamic diameter. , 2009, ACS nano.

[35]  Xiaogang Peng,et al.  Formation of high-quality I-III-VI semiconductor nanocrystals by tuning relative reactivity of cationic precursors. , 2009, Journal of the American Chemical Society.

[36]  M. S. Thakur,et al.  Bioconjugation of CdTe quantum dot for the detection of 2,4-dichlorophenoxyacetic acid by competitive fluoroimmunoassay based biosensor. , 2009, Biosensors & bioelectronics.

[37]  I. Willner,et al.  Semiconductor quantum dots for bioanalysis. , 2008, Angewandte Chemie.

[38]  Andrea Ragusa,et al.  Water solubilization of hydrophobic nanocrystals by means of poly(maleic anhydride-alt-1-octadecene) , 2008 .

[39]  R. Pereiro,et al.  Simple bio-conjugation of polymer-coated quantum dots with antibodies for fluorescence-based immunoassays. , 2008, The Analyst.

[40]  Walter H. Chang,et al.  Design of an amphiphilic polymer for nanoparticle coating and functionalization. , 2008, Small.

[41]  Moungi G Bawendi,et al.  Compact biocompatible quantum dots functionalized for cellular imaging. , 2008, Journal of the American Chemical Society.

[42]  Fusheng Chen,et al.  Preparation for aflatoxin B(1)-cationized bovine serum albumin based on Mannich-type reaction. , 2007, Journal of immunological methods.

[43]  A. Marcus,et al.  Imaging and tracking of tat peptide-conjugated quantum dots in living cells: new insights into nanoparticle uptake, intracellular transport, and vesicle shedding. , 2007, Journal of the American Chemical Society.

[44]  Igor L. Medintz,et al.  Enhancing the stability and biological functionalities of quantum dots via compact multifunctional ligands. , 2007, Journal of the American Chemical Society.

[45]  Shuming Nie,et al.  In Situ Molecular Profiling of Breast Cancer Biomarkers with Multicolor Quantum Dots , 2007 .

[46]  Rebekah Drezek,et al.  Forming biocompatible and nonaggregated nanocrystals in water using amphiphilic polymers. , 2007, Journal of the American Chemical Society.

[47]  Elizabeth L. Bentzen,et al.  Surface modification to reduce nonspecific binding of quantum dots in live cell assays. , 2005, Bioconjugate chemistry.

[48]  M. Bruchez,et al.  Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots , 2003, Nature Biotechnology.

[49]  L. Osterman.  Methods of protein and nucleic acid research , 1984 .