Neurotrophic effect of hepatic growth factor (HGF) on reinnervation of perivascular calcitonin gene-related peptide (CGRP)-containing nerves following phenol-induced nerve injury in the rat mesenteric artery.

Hepatic growth factor (HGF) has neurotrophic effects in the motor neurons and central nervous system. However, there has been no report about the neurotrophic action on perivascular nerves innervating the resistance artery. We investigated whether HGF can restore innervation or function of perivascular nerves, including neuropeptide Y (NPY)-containing sympathetic adrenergic nerves and calcitonin gene-related peptide (CGRP)-containing nerves, in rat mesenteric artery. To investigate HGF-mediated neurotrophic effects, Wistar rats under pentobarbital-Na anesthesia underwent in vivo perivascular denervation by topical application of phenol on the superior mesenteric artery, and then HGF or nerve growth factor (NGF) was administered for 7 days using an osmotic mini-pump after phenol-treatment. HGF significantly increased the density and number of CGRP-like immunoreactivity (LI)-containing nerve fibers compared with saline administration, while HGF did not affect the density of NPY-containing adrenergic nerve fibers. After 7-day treatment with HGF and phenol, the vascular response of vasodilation was recovered from nerve injury by phenol treatment, but vasoconstriction was not. HGF and NGF induced neurite outgrowth in rat cultured dorsal root ganglia (DRG). These results suggest that HGF has a specific neurotrophic action on reinnervation of vascular CGRP-LI-containing nerve fibers in the rat mesenteric artery and DRG.

[1]  H. Kawasaki,et al.  Angiotensin II type 2 receptors facilitate reinnervation of phenol-lesioned vascular calcitonin gene-related peptide–containing nerves in rat mesenteric arteries , 2007, Neuroscience.

[2]  Y. Kitamura,et al.  Adrenomedullin facilitates reinnervation of phenol-injured perivascular nerves in the rat mesenteric resistance artery , 2007, Neuroscience.

[3]  Y. Kitamura,et al.  Innervation and functional changes in mesenteric perivascular calcitonin gene-related peptide- and neuropeptide Y-containing nerves following topical phenol treatment , 2006, Neuroscience.

[4]  Y. Kurosaki,et al.  Long-Term Inhibition of Angiotensin Prevents Reduction of Periarterial Innervation of Calcitonin Gene-Related Peptide (CGRP)-Containing Nerves in Spontaneously Hypertensive Rats , 2005, Hypertension Research.

[5]  J. Timmermans,et al.  In vitro activation of murine DRG neurons by CGRP-mediated mucosal mast cell degranulation. , 2004, American journal of physiology. Gastrointestinal and liver physiology.

[6]  N. Kitamura,et al.  Effect of intracellular dialysis of ATP on the hyperpolarization-activated cation current in rat dorsal root ganglion neurons. , 2003, Journal of neurophysiology.

[7]  Y. Taniyama,et al.  Hepatocyte growth factor prevents endothelial cell death through inhibition of bax translocation from cytosol to mitochondrial membrane. , 2002, Diabetes.

[8]  T. Fukuoka,et al.  Expression of hepatocyte growth factor in primary sensory neurons of adult rats. , 2001, Brain research. Molecular brain research.

[9]  Yi Dai,et al.  Expression of HGF and cMet in the peripheral nervous system of adult rats following sciatic nerve injury , 2001, Neuroreport.

[10]  T. Yamashima,et al.  Analysis of neurotrophic effects of hepatocyte growth factor in the adult hypoglossal nerve axotomy model , 1999, The European journal of neuroscience.

[11]  R. Klein,et al.  Hepatocyte growth factor, a versatile signal for developing neurons , 1999, Nature Neuroscience.

[12]  F. Miller,et al.  Autocrine Hepatocyte Growth Factor Provides a Local Mechanism for Promoting Axonal Growth , 1998, The Journal of Neuroscience.

[13]  M. Aoki,et al.  Negative regulation of local hepatocyte growth factor expression by angiotensin II and transforming growth factor-beta in blood vessels: potential role of HGF in cardiovascular disease. , 1998, Hypertension.

[14]  M. Hanson,et al.  Hepatocyte Growth Factor/Scatter Factor Is an Axonal Chemoattractant and a Neurotrophic Factor for Spinal Motor Neurons , 1996, Neuron.

[15]  M. Aoki,et al.  A vascular modulator, hepatocyte growth factor, is associated with systolic pressure. , 1996, Hypertension.

[16]  T. Nakamura,et al.  Localization and functional coupling of HGF and c-Met/HGF receptor in rat brain: implication as neurotrophic factor. , 1995, Brain research. Molecular brain research.

[17]  A. Saito,et al.  NPY modulates neurotransmission of CGRP-containing vasodilator nerves in rat mesenteric arteries. , 1991, The American journal of physiology.

[18]  T. Westfall,et al.  Inhibition of periarterial nerve stimulation-induced vasodilation of the mesenteric arterial bed by CGRP (8-37) and CGRP receptor desensitization. , 1990, Biochemical and biophysical research communications.

[19]  A. Saito,et al.  Adrenergic modulation of calcitonin gene-related peptide (CGRP)-containing nerve-mediated vasodilation in the rat mesenteric resistance vessel , 1990, Brain Research.

[20]  A. Saito,et al.  Role of calcitonin gene-related peptide-containing nerves in the vascular adrenergic neurotransmission. , 1990, The Journal of pharmacology and experimental therapeutics.

[21]  K. Goto,et al.  Calcitonin gene-related peptide acts as a novel vasodilator neurotransmitter in mesenteric resistance vessels of the rat , 1988, Nature.

[22]  S. Hirono,et al.  Human hepatocyte growth factor in plasma from patients with fulminant hepatic failure. , 1986, Experimental cell research.

[23]  H. Tsubouchi,et al.  STIMULATION OF DNA SYNTHESIS IN ADULT RAT HEPATOCYTES IN PRIMARY CULTURE BY SERA FROM PATIENTS WITH FULMINANT HEPATIC FAILURE , 1985 .