Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro

The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r = 0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach.

[1]  G. Dasch,et al.  Development of a quantitative real-time polymerase chain reaction assay specific for Orientia tsutsugamushi. , 2004, The American journal of tropical medicine and hygiene.

[2]  S. M. Lee,et al.  Neutralization epitopes on the antigenic domain II of the Orientia tsutsugamushi 56-kDa protein revealed by monoclonal antibodies. , 2000, Vaccine.

[3]  S. Seong,et al.  Induction of neutralizing antibody in mice by immunization with recombinant 56 kDa protein of Orientia tsutsugamushi. , 1997, Vaccine.

[4]  M. Moree,et al.  Growth characteristics and proteins of plaque-purified strains of Rickettsia tsutsugamushi , 1992, Infection and immunity.

[5]  M L Mendelsohn,et al.  THE ANALYSIS OF CELL IMAGES * , 1966, Annals of the New York Academy of Sciences.

[6]  P. van Oostveldt,et al.  Digital titration: Automated image acquisition and analysis of load and growth of Chlamydophila psittaci , 2009, Microscopy research and technique.

[7]  J. Kang,et al.  Improved Antibiotic Susceptibility Test of Orientia tsutsugamushi by Flow Cytometry Using Monoclonal Antibody , 2007, Journal of Korean medical science.

[8]  N. Day,et al.  A highly sensitive quantitative real-time PCR assay based on the groEL gene of contemporary Thai strains of Orientia tsutsugamushi , 2009, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases.

[9]  Shyang Chang,et al.  A new criterion for automatic multilevel thresholding , 1995, IEEE Trans. Image Process..

[10]  Andrew K. C. Wong,et al.  A new method for gray-level picture thresholding using the entropy of the histogram , 1985, Comput. Vis. Graph. Image Process..

[11]  D. Strickman,et al.  Scrub typhus infections poorly responsive to antibiotics in northern Thailand , 1996, The Lancet.

[12]  D. Strickman,et al.  Rickettsia tsutsugamushi infection in cell culture: antibiotic susceptibility determined by flow cytometry. , 1995, The American journal of tropical medicine and hygiene.

[13]  J. Frean,et al.  Reliable enumeration of malaria parasites in thick blood films using digital image analysis , 2009, Malaria Journal.

[14]  N. Day,et al.  Rapid diagnosis of scrub typhus in rural Thailand using polymerase chain reaction. , 2006, The American journal of tropical medicine and hygiene.

[15]  B. Hanson.  Improved plaque assay for Rickettsia tsutsugamushi. , 1987, The American journal of tropical medicine and hygiene.

[16]  C. Chao,et al.  Scrub Typhus Vaccine Candidate Kp r56 Induces Humoral and Cellular Immune Responses in Cynomolgus Monkeys , 2005, Infection and Immunity.