Nutritional evaluation of the legume Macrotyloma axillare using in vitro and in vivo bioassays in sheep

This study consisted of two experiments with the following objectives: to evaluate the effects of tannins from the tropical legume macrotiloma (Macrotyloma axillare) on total gas and methane (CH4 ) production, as well as on ruminal fermentation parameters by performing an in vitro bioassay, with samples incubated with and without polyethylene glycol (PEG) in a semi-automatic system; and secondly in a 17 day in vivo experiment, to determine apparent total tract digestibility (ATTD) of dietary nutrients and ruminal fermentation parameters of 12 intact 8- to 9-month-old Santa Inês (averaging 24.95 ± 1.8 kg body weight) ewes fed tropical grass hay supplemented with macrotiloma hay. The ewes were divided into two treatment groups depending on their diet: chopped aruana grass hay (Panicum maximum cv. Aruana) (control-CON); and aruana grass hay supplemented with chopped macrotiloma hay (macrotiloma-MAC). The animals were kept for 5 consecutive days in metabolic cages for the ATTD assay, and at the end of this period, samples of rumen fluid were collected from each ewe to determine ammoniacal nitrogen (NH3 -N) and short-chain fatty acid (SCFA) production, and protozoa count. For the in vitro assay, a decrease in total gas and CH4 production was observed for samples incubated without PEG (p < .05). No differences were observed for the other parameters evaluated (p > .05). In the in vivo experiment, increased intake and ATTD of crude protein were observed for the animals fed MAC when compared to CON (p < .05). For rumen fermentation parameters, increased NH3 -N, total SCFA and isobutyrate concentrations, as well as reduced protozoa count were observed for MAC when compared to CON (p < .05). The results observed here indicated the potential of macrotiloma for use as a ruminant feed, and antimethanogenic potential of this plant was noted.

[1]  R. Hegarty,et al.  Methane emissions, ruminal characteristics and nitrogen utilisation changes after refaunation of protozoa-free sheep , 2016 .

[2]  R. Bhatta,et al.  Effects of graded levels of tannin‐containing tropical tree leaves on in vitro rumen fermentation, total protozoa and methane production , 2015, Journal of applied microbiology.

[3]  W. Hendriks,et al.  Chemical composition and in vitro total gas and methane production of forage species from the Mid Rift Valley grasslands of Ethiopia , 2014 .

[4]  M. Fondevila,et al.  Biological effect of tannins from different vegetal origin on microbial and fermentation traits in vitro , 2014 .

[5]  P. Gerber,et al.  Special topics--Mitigation of methane and nitrous oxide emissions from animal operations: I. A review of enteric methane mitigation options. , 2013, Journal of animal science.

[6]  M. Kreuzer,et al.  Contribution of condensed tannins and mimosine to the methane mitigation caused by feeding Leucaena leucocephala , 2013, Archives of animal nutrition.

[7]  D. Andueza,et al.  Mixing sainfoin and lucerne to improve the feed value of legumes fed to sheep by the effect of condensed tannins. , 2013, Animal : an international journal of animal bioscience.

[8]  E. Ørskov,et al.  Effects of Tropical High Tannin Non Legume and Low Tannin Legume Browse Mixtures on Fermentation Parameters and Methanogenesis Using Gas Production Technique , 2012, Asian-Australasian journal of animal sciences.

[9]  J. Neiva,et al.  Parâmetros da fermentação ruminal e concentração de derivados de purina de vacas em lactação alimentadas com castanha de caju , 2012 .

[10]  M. Kreuzer,et al.  Meta-analysis of the relationship between dietary tannin level and methane formation in ruminants from in vivo and in vitro experiments. , 2012, Journal of animal physiology and animal nutrition.

[11]  H. Hoste,et al.  Direct and indirect effects of bioactive tannin-rich tropical and temperate legumes against nematode infections. , 2012, Veterinary parasitology.

[12]  R. Bhatta,et al.  Methane reduction and energy partitioning in goats fed two concentrations of tannin from Mimosa spp. , 2012, The Journal of Agricultural Science.

[13]  H. Makkar,et al.  Methane mitigation from ruminants using tannins and saponins , 2011, Tropical Animal Health and Production.

[14]  P. Lecomte,et al.  Comparison of methane production between C3 and C4 grasses and legumes , 2011 .

[15]  G. Waghorn,et al.  Effects of feeding fresh white clover (Trifolium repens) or perennial ryegrass (Lolium perenne) on enteric methane emissions from sheep , 2011 .

[16]  G. B. Mourão,et al.  Use of blanks to determine in vitro net gas and methane production when using rumen fermentation modifiers , 2011 .

[17]  B. McBride,et al.  Methanogens: Methane Producers of the Rumen and Mitigation Strategies , 2010, Archaea.

[18]  B. Dijkstra,et al.  Two Major Archaeal Pseudomurein Endoisopeptidases: PeiW and PeiP , 2010, Archaea.

[19]  E. Detmann,et al.  Intake and digestibility in cattle fed low-quality tropical forage and supplemented with nitrogenous compounds , 2010, Tropical Animal Health and Production.

[20]  F. López-González,et al.  RUMINAL FERMENTATION AND TANNINS BIOACTIVITY OF SOME BROWSES USING A SEMI-AUTOMATED GAS PRODUCTION TECHNIQUE , 2010 .

[21]  W. Horwitz,et al.  Official methods of analysis of AOAC International , 2010 .

[22]  M. Kreuzer,et al.  Effect of season, soil type and fertilizer on the biomass production and chemical composition of five tropical shrub legumes with forage potential , 2009 .

[23]  M. Kreuzer,et al.  Effect of the tropical tannin-rich shrub legumes Calliandra calothyrsus and Flemingia macrophylla on methane emission and nitrogen and energy balance in growing lambs. , 2008, Animal : an international journal of animal bioscience.

[24]  D. Veira,et al.  Comparison of alfalfa and mixed alfalfa-sainfoin pastures for grazing cattle: Effects on incidence of bloat, ruminal fermentation, and feed intake , 2006 .

[25]  I. C. Bueno,et al.  The influence of head-space and inoculum dilution on in vitro ruminal methane measurements , 2006 .

[26]  M. J. Valarini,et al.  Research note: Nutritive value of a range of tropical forage legumes , 2006 .

[27]  I. C. Bueno,et al.  Influence of inoculum source in a gas production method , 2005 .

[28]  N. López-Villalobos,et al.  Use of Lotus corniculatus containing condensed tannins to increase reproductive efficiency in ewes u , 2005 .

[29]  D. Mertens.  Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study. , 2002, Journal of AOAC International.

[30]  F. Giráldez,et al.  Condensed tannin content of several shrub species from a mountain area in northern Spain, and its relationship to various indicators of nutritive value , 2002 .

[31]  M. Theodorou,et al.  A semi-automated in vitro gas production technique for ruminant feedstuff evaluation , 1999 .

[32]  P. Cunniff.  Official Methods of Analysis of AOAC International , 2019 .

[33]  K. Becker,et al.  Formation of complexes between polyvinyl pyrrolidones or polyethylene glycols and tannins, and their implication in gas production and true digestibility in in vitro techniques , 1995, British Journal of Nutrition.

[34]  M. Theodorou,et al.  A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. , 1994 .

[35]  P. V. Soest,et al.  Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. , 1991, Journal of dairy science.

[36]  R. Leng.  Factors Affecting the Utilization of ‘Poor-Quality’ Forages by Ruminants Particularly Under Tropical Conditions , 1990, Nutrition Research Reviews.

[37]  B. A. Dehority,et al.  Occurrence of the Rumen Ciliate Oligoisotricha bubali in Domestic Cattle (Bos taurus) , 1983, Applied and environmental microbiology.

[38]  D. Palmquist,et al.  Origin of plasma fatty acids in lactating cows fed high grain or high fat diets. , 1971, Journal of dairy science.

[39]  Biological and chemical analytical methods , 2022 .