A method for synthesis and functionalization of ultrasmall superparamagnetic covalent carriers based on maghemite and dextran

Abstract A new generation of susceptibility contrast agents for MRI and based on maghemite cores covalently bonded to dextran stabilizing macromolecules was investigated. The multistep preparation of these versatile ultrasmall superparamagnetic iron oxides (VUSPIO) consisted of colloidal maghemite synthesis, surface modification by aminopropylsilane groups, and coupling of partially oxidized dextran via Schiff's bases and secondary amine bonds. The dextran corona might be easily derivatized, e.g. by PEGylation.

[1]  R. Massart,et al.  Preparation of aqueous magnetic liquids in alkaline and acidic media , 1981 .

[2]  R. Brooks,et al.  Relaxometry and magnetometry of the MR contrast agent MION‐46L , 1999, Magnetic resonance in medicine.

[3]  C. W. Jung.  Surface properties of superparamagnetic iron oxide MR contrast agents: ferumoxides, ferumoxtran, ferumoxsil. , 1995, Magnetic resonance imaging.

[4]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[5]  M Martignoni,et al.  [Silane coupling agents]. , 1990, Attualita dentale.

[6]  S M Moghimi,et al.  Long-circulating and target-specific nanoparticles: theory to practice. , 2001, Pharmacological reviews.

[7]  A. Fischman,et al.  Polyclonal human immunoglobulin G labeled with polymeric iron oxide: antibody MR imaging. , 1991, Radiology.

[8]  Ralph Weissleder,et al.  Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells , 2000, Nature Biotechnology.

[9]  E. Plueddemann,et al.  SILANE COUPLING AGENTS , 1982 .

[10]  E. Groman,et al.  Synthesis of ultrasmall superparamagnetic iron oxides using reduced polysaccharides. , 2004, Bioconjugate chemistry.

[11]  R Weissleder,et al.  MR of carcinoma-specific monoclonal antibody conjugated to monocrystalline iron oxide nanoparticles: the potential for noninvasive diagnosis. , 1996, AJNR. American journal of neuroradiology.

[12]  Ericka Stricklin-Parker,et al.  Ann , 2005 .

[13]  W Semmler,et al.  Targeting of ultrasmall superparamagnetic iron oxide (USPIO) particles to tumor cells in Vivo by using transferrin receptor pathways , 1998, Magnetic resonance in medicine.

[14]  B. Bonnemain,et al.  Superparamagnetic agents in magnetic resonance imaging: physicochemical characteristics and clinical applications. A review. , 1998, Journal of drug targeting.

[15]  É. Duguet,et al.  Magnetic nanoparticle design for medical diagnosis and therapy , 2004 .

[16]  Yoshimi Anzai,et al.  MR angiography with an ultrasmall superparamagnetic iron oxide blood pool agent , 1997, Journal of magnetic resonance imaging : JMRI.

[17]  É. Duguet,et al.  PMMA-based composite materials with reactive ceramic fillers. Part1.—Chemical modification and characterisation of ceramicparticles , 1997 .

[18]  J. Koenig,et al.  The structure of γ-aminopropyltriethoxysilane on glass surfaces , 1980 .

[19]  R Weissleder,et al.  MR lymphography: study of a high-efficiency lymphotrophic agent. , 1994, Radiology.